三个鲤品种线粒体基因片段序列保守性

童金苟, 吴清江

童金苟, 吴清江. 三个鲤品种线粒体基因片段序列保守性[J]. 水生生物学报, 2001, 25(1): 54-60.
引用本文: 童金苟, 吴清江. 三个鲤品种线粒体基因片段序列保守性[J]. 水生生物学报, 2001, 25(1): 54-60.
TONG Jin-gou, WU Qing-jiang. SEQUENCE CONSERVATION ON SEGMENTS OF MITOCHONDRIAL 16S rRNA AND CYTOCHROME B IN STRAINS OF COMMON CARP(CYPRINUS CARPIO L. VAR.)[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(1): 54-60.
Citation: TONG Jin-gou, WU Qing-jiang. SEQUENCE CONSERVATION ON SEGMENTS OF MITOCHONDRIAL 16S rRNA AND CYTOCHROME B IN STRAINS OF COMMON CARP(CYPRINUS CARPIO L. VAR.)[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(1): 54-60.

三个鲤品种线粒体基因片段序列保守性

基金项目: 

国家自然科学基金项目(批准号:39970587)

SEQUENCE CONSERVATION ON SEGMENTS OF MITOCHONDRIAL 16S rRNA AND CYTOCHROME B IN STRAINS OF COMMON CARP(CYPRINUS CARPIO L. VAR.)

  • 摘要: 在红鲤、镜鲤和野鲤中进行线粒体16SrRNA和Cytb基因片段的序列测定.结果发现,3个鲤品种在多达859个碱基长度上无变异,但是与已报道的鲤全序列中的同源序列则有约15%的趋异.这个结果提示3个鲤品种可能在起源中是独立的一支,并且分化极低.
    Abstract: Mitochondrial DNA sequence analysis was carried out in 3 strains of Chinese carp(Cyprinus carpio L. var.), i.e.red carp, mirror carp and wild carp and compared with reference carp strain of unknown origin. There were no base substitutions among 3 strains at up to 859 base pairs, however, comparison between these sequences and homologous sequence of the reference strain showed about 0.19% and 1.46% divergence at 16S rRNA and Cytochrome b sequence, respectively. These results indicate that the genetic divergence among red carp, mirror carp and wild carp is exceptionally low, and they are probably an independent branch in the evolution of carp.
  • [1] Chang Y S, Huang F L, Lo T B.Complete nucleotide sequences and gene arrangement of carp mitochondrial genome [J].J Mol Evol, 1994, 38:138—155[2] Avise J C.Molecular markers, natural history and evolution [M].New York: Chapman and Hall, 1994[3] Kocher T D, Thomas W K, Meyer A, et al.Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers [J].Proc Natl Acad Sci USA, 1989, 86:6196—6200[4] Saiki R, Gelfand D H, Stoffel S et al.Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase [J].Science, 1988, 239:487—491[5] Sanger F, Nicklen S, Coulson A R.DNA sequencing with chain-terminating inhibitors [J].Proc Natl Acad Sci USA, 1977A, 74:5463—5467[6] Brown W M, George M Jr., Wilson A C.Rapid evolution of animal mitochondrial DNA [J].Proc Natl Acad Sci USA, 1979, 76:1967—1971[7] Harrison R G.Animal mitochondrial DNA as a genetic marker in population and evolutionary biology [J].Trend Ecol Evol, 1989, 4:6—11[8] Hillis D M, Moritz C, Bable B.Molecular Systematics 2nd etition [M].Sunderland: Sinauer, 1996[9] Sarver S K, Silberman J D, Walsh P J.Mitochondrial DNA sequence evidence supporting the recognition of two subspecies or species of Florida spiny lobster Panulirus argus [J].J Crustacean Biol, 1998, 18:177—186[10] Palumbi S R, Benzie J.Large mitochondrial differences betweens morphologically similar penaeid shrimp[J].Mol Mar Biol Biotechn, 1991, 1:27—34[11] Zhang D X, Hewitt G M.Nuclear integrations: Challenges for mitochondrial DNA markers [J].Trend Ecol Evol, 1996, 11:247—251[12] Whitmore D H, Thai T H, Craft C M.The largemouth bass cytochrome b gene [J].J Fish Biol, 1994, 44:637[13] Carr S M, Marshall H D.Detection of intraspecific DNA sequence variation in the mitochondrial cytochrome b gene of Atlantic cod(Gadus morhua)by the polymerase chain reaction [J].Can J Fish Aquat Sci, 1991, 48:48—52[14] 伍献文等.鲤科鱼类志[M].上海:上海科学技术出版社,1981

    Chang Y S, Huang F L, Lo T B.Complete nucleotide sequences and gene arrangement of carp mitochondrial genome [J].J Mol Evol, 1994, 38:138—155[2] Avise J C.Molecular markers, natural history and evolution [M].New York: Chapman and Hall, 1994[3] Kocher T D, Thomas W K, Meyer A, et al.Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers [J].Proc Natl Acad Sci USA, 1989, 86:6196—6200[4] Saiki R, Gelfand D H, Stoffel S et al.Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase [J].Science, 1988, 239:487—491[5] Sanger F, Nicklen S, Coulson A R.DNA sequencing with chain-terminating inhibitors [J].Proc Natl Acad Sci USA, 1977A, 74:5463—5467[6] Brown W M, George M Jr., Wilson A C.Rapid evolution of animal mitochondrial DNA [J].Proc Natl Acad Sci USA, 1979, 76:1967—1971[7] Harrison R G.Animal mitochondrial DNA as a genetic marker in population and evolutionary biology [J].Trend Ecol Evol, 1989, 4:6—11[8] Hillis D M, Moritz C, Bable B.Molecular Systematics 2nd etition [M].Sunderland: Sinauer, 1996[9] Sarver S K, Silberman J D, Walsh P J.Mitochondrial DNA sequence evidence supporting the recognition of two subspecies or species of Florida spiny lobster Panulirus argus [J].J Crustacean Biol, 1998, 18:177—186[10] Palumbi S R, Benzie J.Large mitochondrial differences betweens morphologically similar penaeid shrimp[J].Mol Mar Biol Biotechn, 1991, 1:27—34[11] Zhang D X, Hewitt G M.Nuclear integrations: Challenges for mitochondrial DNA markers [J].Trend Ecol Evol, 1996, 11:247—251[12] Whitmore D H, Thai T H, Craft C M.The largemouth bass cytochrome b gene [J].J Fish Biol, 1994, 44:637[13] Carr S M, Marshall H D.Detection of intraspecific DNA sequence variation in the mitochondrial cytochrome b gene of Atlantic cod(Gadus morhua)by the polymerase chain reaction [J].Can J Fish Aquat Sci, 1991, 48:48—52[14] 伍献文等.鲤科鱼类志[M].上海:上海科学技术出版社,1981

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出版历程
  • 收稿日期:  1998-12-27
  • 修回日期:  2000-09-09
  • 发布日期:  2001-01-24

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