不同游泳速度条件下瓦氏黄颡幼鱼的有氧和无氧代谢反应
AEROBIC AND ANAEROBIC METABOLISM IN RESPONSE TO DIFFERENT SWIMMING SPEED OF JUVENILE DARKBARBEL CATFISH (PELTEOBAGRUS VACHELLI RICHARDSON)
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摘要: 在(25±1)℃的条件下,测定瓦氏黄颡(Pelteobagrus vachelli Richardson)幼鱼[体重(4.34±0.13)g]的临界游泳速度(Ucrit),然后分别以临界游泳速度的不同百分比(20、40、60、80、100%Ucrit)将实验鱼分为5个速度处理组,另外设置静止对照组和高速力竭对照组。处理组实验鱼在不同游泳速度下分别游泳20min,在此过程中测定并计算运动代谢率(Activity metabolic rate,AMR),随后测定肌肉、血液和肝脏中的乳酸、糖原和葡萄糖含量。结果显示:实验鱼的绝对临界游泳速度为(48.28±1.02)cm/s,相对临界游泳速度为(6.78±0.16)BL/s;随着游泳速度的提高AMR显著增加(Pcrit时肌乳酸和血乳酸含量显著高于80%Ucrit的水平(P0.05);100%Ucrit时肝糖原含量显著低于40%Ucrit的水平(P0.05)。经计算瓦氏黄颡幼鱼到达临界游泳速度时的无氧代谢功率比例仅为11.0%,表明其游泳运动主要以有氧代谢供能;实验鱼的无氧代谢大约在80%Ucrit才开始启动,与其他鱼类比较启动时间较晚,说明其游泳运动对无氧代谢的依赖程度较低。研究提示瓦氏黄颡幼鱼是一种有氧运动能力较强的鱼类,这一能量代谢特征可能与提高其生存适合度有关。Abstract: The aim of this study were, to determine the anaerobic to aerobic metabolism ratio under the maximum sus-tainable swimming speed (critical swimming speed, Ucrit) of selected fish species, to examine at what swimming speed fish start to recruit its anaerobic metabolism, and hence to investigate the relationship among locomotion performance, energy supply characteristics and ecological habits of selected experimental animal. Juvenile darkbarbel catfish (Pelteobagrus vachelli Richardson) [w: (4.34±0.13) g], an economic fish species distribute widely in the Yangtze River and Pearl River, were chosen as the experimental animal. The critical swimming speed (Ucrit) of juvenile Darkbarbel Catfish was determined at (25 ± 1)℃. Based on the Ucrit data, we set 7 experimental groups: 2 control groups (resting control group and high-speed exhaustive control group) and 5 speed-manipulating groups, which were set following the gradient of mean Ucrit (20, 40, 60, 80 and 100% Ucrit). Fish of speed-manipulating groups were forced to swim for 20 minutes at its setting speed, during this period, the oxygen consumption rate was measured and the activity metabolic rate was calculated. The lactate, glycogen and glucose levels of muscle, blood and liver of fish in experimental treatment groups were determined immediately after forced swimming. The biochemical parameters and resting metabolic rate (only for resting control group) were also measured in two control groups. The absolutely critical swimming speed of juvenile darkbarbel catfish in this study was (48.28±1.02) cm/s and the relative critical swimming speed was (6.78±0.16) BL/s. The oxygen consumption rate raised significantly with the increasing of the swimming speed (P 0.05). The mus-cle and blood lactate levels of fish underwent 100% Ucrit treatment were (7.25±0.70) μmol/g and (9.25±2.66) mmol/L while the lactate levles of muscle and blood samples were (5.31±0.43) μmol/g and (3.44±0.25) mmol/L, respectively. The lactate levels of both muscle and blood samples of 100% Ucrit treatment group were significantly higher than those of 80% Ucrit treatment group (P 0.05) while the lactate level of liver showed no significant change (P 0.05); As swimming speed increased, glycogen content of all three tissues showed a downward trend (P 0.05), of which liver glycogen content of 100% Ucrit group was significantly lower than that of 40% Ucrit level (P 0.05) while glycogen content of muscle showed no significant difference among all experimental treatment groups (P 0.05). Glucose levels remained relatively stable. Calculated anaerobic metabolism to aerobic metabolism power ratio was 11.0% when juve-nile Darkbarbel Catfish reached the critical swimming speed, indicating that the main power came from aerobic me-tabolism path; anaerobic metabolism started its function when fish reached around 80% of its Ucrit, the start-up time was late comparing with other fish. It showed that their swimming activity relied less on anaerobic metabolism. This study indicates that juvenile Darkbarbel Catfish has strong aerobic capacity and the characteristics of energy metabolism in this fish species may be related to its higher survival fitness.
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Keywords:
- Critical swimming speed /
- Darkbarbel Catfish /
- Oxygen consumption rate /
- Lactate /
- Glycogen
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[1] Kieffer J D.Limits to exhaustive exercise in fish[J].Comparative Biochemistry and Physiology,2000,126A:161-179
[2] Franklin C E,Wilson R S,Davison W.Locomotion at-1.0℃:burst swimming performance of five species of Antarctic fish[J].Journal of Thermal Biology,2003,28:59-65
[3] Baldwin J,Seymour R S,Webb G J W.Scaling ofauserobic metabolism during exercise in the estuarine crocodile(Crocodylus porosus)[J].Comparative Biochemistry and Physiology,1995,112A:285-293
[4] Virani N A,Rees B B.Oxygen consumption,blood lactate and interindividnal variation in the gulf kilifish,Fundulus grandis,during hypoxia and recovery[J].Comparative Biochemistry and Physiology,2000,126A:397-405
[5] Day N,Butler P J.The effects of acclimation to reversed seasonal temperature on the swimming performance of adult brown trout Salmo trutta[J].The Journal of Experimental Biology,2005,208:2683-2692
[6] Reidy S P,Kerr S R,Nelson J A.Aerobic and anaerobic swimming performance of individual Atlantic Cod[J].The Journal of Experimental Biology,2000,203:347-357
[7] Rome L C,Louglma P T,Goldspink G.Muscle fibre activity in carp as a function of swimming speed and muscle temperature[J].American Journal of Physiology,1984,247R:272-279
[8] Burgetz I J,Rojas-vargas A,Hinch S G,et al.Initial recruitment of anaerobic metabolism during submaximal swimming in rainbow trout(Oncorhynchus mykiss)[J].The Journal of Experimental Biology,1998,201:2711-2721
[9] Webb P W.The swimming energetics of trout.Ⅱ.Oxygen consumption and swimming efficiency[J].The Journal of Experimental Biology,1971,55:521-540
[10] Lee C G,Farrell A P,Lotto A,et al.Excess post-exercise oxygen consumption in adult sockeye(Oncorhynchus nerka)and coho(O.kisutch)salmon following critical speed awimming[J].The Journal of Experimental Biology,2003,206:3253-3260
[11] Farrell A P.Cardiorespiratory performance during prolonged swimming tests with salmonids:a perspective on temperature effects and potential analytical pitfalls[J].Philosophical Transactions of the Royal Society,2007,362B:2017-2030
[12] Fu S J,Cao Z D,Peng J L.The effect of chasing and air exposing oxygen consumption in Pelteobag vachelli[J].Chinese Journal of Zoology,2007,42(2):111-115[付世建,曹振东,彭姜岚.追赶和空气曝露时间对瓦氏黄颡鱼耗氧率的影响.动物学杂志,2007,42(2):111-115]
[13] Li W X,Wang G T,Wu S G,et al.Population dynamics and frequency distribution of the nematode Procamallanus fulvidraconis and trematode Genarchopsis goppo in yellow-head catfish Pelteobagrus fulvidroco[J].Acta Hydrobiologica Sinica,2008,32(2):195-200[李文祥,王桂堂,吴山功,等.黄颡鱼河鲈源吸虫和黄颡前鸵形线虫的季节动态和频率分布.水生生物学报,2008,32(2):195-200]
[14] Zhao W X,Yang X,Peng Z,et al.Molecular identification of the four species of the genus Peheobagrus and the genetic analysis of their hybrid[J].Acta Hydrobiologica Sinica,2006,30(1):101-106[赵文学,杨星,彭智,等.黄颡鱼属物种的RAPD分子鉴定及杂种遗传分析.水生生物学报,2006,30(1):101-106]
[15] Liu Y,Cao Z D,Fu S J,et al.The effect of exhaustive chasing training and detraining on swimming performance in juvenile darkbarbel catfish(Peltebagrns vachelli)[J].Journal Comparative Physiology,2009,179B:847-855
[16] Zhang Y,Cao Z D,Fu S J.Effect delayed first feeding on the energy content,body length and swimming performance of southern catfish(Silurus meridionalis Chen)larvae[J].Acta Ecological Sinica,2007,27(3):1161-1167[张冶,曹振东,付世建.延迟首次投喂对南方鲇(Silurus meridionalis Chen)仔鱼身体含能量,体长及游泳能力的影响.生态学报,2007,27(3):1161-1167]
[17] Jain K E,Hamilton J C,Farrell A P.Use of a ramp velocity test to measure critical swimming speed in Rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry Physiology,1997,117A:441-444
[18] Jain K E,Farrell A P.Influence of seasonal temperature on the repeat swimming performance of rainbow trout Oncorhynchus mykiss[J].The Journal of Experimental Biology,2003,206:3569-3579
[19] Lee C G,Farrell A P,Lotto A,et al.The effect of temperature on swimming performance and oxygen consumption in adult sockeye(Oncorhynchus nerka)and coho(O.kisutch)salmon stocks[J].The Journal of Experimental Biology,2003,206:3239-3251
[20] Derek H A,Chris M W.The interactive effects of feeding and exercise on oxygen consumption,swimming performance and protein usage in Juvenile rainbow trout(Oncorhynchus mykiss)[J].The Journal of Experimental Biology,1997,200:2337-2346
[21] Pang X,Can Z D,Peng J L,Fu S J.The effects of feeding on the swimming performance and metabolic response of juvenile southern catfish,Silurus meridionalis,acclimated at different temperatures[J].Comparative Biochemistry and Physiology,2010,155A:253-258
[22] Claireaux G,Couturier C,Groison A.Effect of temperature on maximum swimming speed and cost of transport in juvenile European sea bass(Dicentrarchus labrax)[J].The Journal of Experimental Biology,2006,209:3420-3428
[23] Luo Y P,Yuan L Q,Cao Z D,et al.The study on haematological parameters of Mystus macropterus and Pelteobagrus vachellii in Jialing River[J].Acta Hydrobiologica Sinica,2005,29(2):161-166[罗毅平,袁伦强,曹振东,等.嘉陵江大鳍鱯和瓦氏黄颡鱼血液指标的研究.水生生物学报,2005,29(2):161-166]
[24] Zhang L X,Zhang T F,Li L Y.Biochemistry experiments and technology[M].Beijing:High Education Press.1997,422-427[张龙翔,张庭芳,李令媛.生化实验方法和技术.北京:高等教育出版社.1997,422-427]
[25] Blasco J,Marimon I,Viaplana I,et al.Fate of plasma glucose in tissues of brown trout in vivo:effects of fasting and glucose loading[J].Fish Physiology and Biochemistry,2001,24:247-258
[26] Fitzgibbon Q p.Strawbridge A,Seymour R S.Metabolic scope,swimming performance and the effects of hypoxia in the mulloway,Argyrosomus japonicus(Pisces:Sciaenidae)[J].Aquaculture,2007,270:358-368
[27] Reidy S P,Nelson J A,Tang Y,et al.Post-exercise metabolic rate in Atlantic cod and its dependence upon the method of exhaustive[J].Journal of Fish Biology,1995,47:377-386
[28] Bushnell P G,Steffensen J F,Johansen K.Oxygen consumption and swimming performance in hypoxia-acclimated rainbow trout Salmo gairdneri[J].The Journal of Experimental Biology,1984,113:225-235
[29] Peng J L,Cao Z D,Fu S J.Excess post-exercise oxygen consumption in Silurus asotus Linnaeus and its relationship with body weight[J].Acta Hydrobiologica Sinica,2008,32(3):380-386[彭姜岚,曹振东,付世建.鲇鱼力竭性运动后的过量耗氧及其体重的影响.水生生物学报,2008,32(3):380-386]
[30] Goolish E M.The scaling of aerobic and anaerobic muscle power in rainbow trout(Salmo gairdneri)[J].The Journal of Experimental Biology,1989,147:493-505
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