两种鳜病毒的共感染现象及引起感染细胞的超微变化
COINFECTION OF TWO SINIPERCA CHUATSI VIRUSES AND MORPHOLOGIC CHANGES OF THE INFECTED CELLS
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摘要: 借助细胞培养和电镜技术,揭示了鳜球形病毒(Siniperca chuatsi spherical virus,SCSV)与鳜弹状病毒(Siniperca chuatsi rhabdovirus,SCRV)在草鱼鳍细胞(Grass carp fins,GCF)中共感染的现象。在筛选到敏感鱼类细胞系和建立了 鳜病毒体外增殖系统的基础上,取息典型病毒感染出血症的鳜组织,制备组织悬液,接种到GCF细胞中传代培养, 在攻毒后间隔不同时间收集细胞,对攻毒细胞的超薄切片进行电镜观察。揭示两种形态的鳜病毒可在同一个GCF 细胞中增殖,并描述和分析了病毒复制引起感染细胞的超微病变。本研究结果有助于阐明鱼类重要病毒病害的发 生过程及致病机理。Abstract: Serious economic losses in the aquaculture industry have been resulted from viral diseases in China as well as the worldwide. Mandarin fish Siniperca chuatsi is a special aquaculture fish in China. Siniperca chuatsi spheriacal virus (SCSV) has been demonstrated to be pathogen by a series of experiments such as electron microscope observation, cell challenge, host fish infection and histopathological investigation.However,the epizootic disease of mandarin fish could not be efficiently controlled by the viral vaccine. Different kinds of viruses were observed from the tissues of diseased mandarin fish with outbreak of infection and acute lethality. This study reveals two kinds viruses, Siniperca chuatsi spherical virus(SCSV) and Siniperca chuatsi rhabdovirus (SCRV) from diseased mandarin fish coinfected in vitro. The samples of viruses were prepared from tissues of the diseased mandarin fish with typical hemorrhage syndrome.The fish cell line of grass carp fins (GCF) was used to amplify the viruses.The infected GCF cells were collected at different time after infection, and the ultra thin section were cut, stained and examined by transmission electron microscopy. Two kinds of different viruses were observed, one is spherical virus and other is rhabdovirus. The morphogenesis ,coinfections and cellular interactions with GCF cells were investigated, such as the virus amplification in cytoplasm of infected cells,budding from the plasma membranes,and damages of the cells.These results may help explain the fact that different virus species are responsible for significant disease problems and pathogenesis in cultured fishes.
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[1] Zhang Q Y. A review of virel diseases of aquatic animal in China [J.]. Actor drobiology Sinica, 2002, 26 (1) : 89-101[张奇亚.我国水生动物病毒病研究概况.水生生物学报2002.26(1):93-101]
[2] Zhang Q Y, Li Z Q. Three different viruses observed from the tissues of diseased mandarin fsh Siniperrn chuatsi[J.] Chinese Science Bulletin.1999.44( 5 ) :437-440 [张奇亚，李正秋，在患病撅鱼组织中观察到三种病毒.科学通报，1999.44(2):192- 195]
[3] Zhang Q Y, Li Z Q, Detection of vital pathogen from diseased mandarin fish( Siniperca chuatsi )with pathological observation[J],Actahydrobiobgica sinirn 1999. 23 ( 2 ) : 151-154 [张奇亚.李正秋，1999.级鱼病毒病原的检出及组织病理分析.水生生物学报. 13(2):I51一154]
[4] Lu Y, lannan C N, Rohovec J S, et al, Fish cell lines: Establishment and cheracteriration of three new cell linea from grass carp (Ctenopltaryngodon idellus ). In Vitro [1].Cell Deu Biol，1990.26: 275-279
[5] Betts A M,Stone D M. Rescue of Viral haemorrhagic septicaemie virus minigenomes by helper virus[J]. Virus Rea 2001，77(1):19-23
[6] Biecchesi S, Yu Y X. Bearzotti M, et al. Rescue of synthetic salmonid rhabdovirus minigenomes [J],J Gen Virol，2000. 81(Pt8 ):1941-1945
[7] Alonso M. Rodriguez S, Perez-Prieto SI. Viral coinfection in salmonida: infectious pancreatic necrosis virus interferon with infecnoun hematopoietic necrosis virus [J]. Arch Virol. 1999, 144 ( 4 ): 657-673
[8] Nee S, Mutuaiiam, parasitism and competition in the evolution of covitvsea[J].Philoa Tram R Soc fond B Biol Sci. 2000. 355 (1403 ): 1607-1613
[9] Scholtiseek C. Stech J, Krausa S, et al. Cooperation between the hemagglutinin of avian vintsee and the matrix protein of human influenze A viruses[J].J. Virol 2002,76(4):1781一1786
[10] Flotte T R. Carter H J. Adeno-aaeociated vista vectorn for gene therapy[J]. Cone Ther.1995,2:357-362
[11] Hillgenberg M. Schniedets F, Lneer P. et al，System for efficient helper-dependent minimal adenovirue construction and rescue [J],Hum Gene 7Rer.2001.12(6):643-657
[12] Ehrhardt A, Kay M A.A new adenoviral helper-dependent vector，results in long-term therapeutic levels of human coagulation factor IX at low doses in vivo [J].Blood, 2002.99(11):3923-3930
[13] Sena-Esteves M,Hampl J A，Camp S M. et al. Generation of stable retrovirus packaing cell lines after treneduction with herpes simplex virus hybrid amplicon vectorsdouble dagger[J]. Gene Med.2002. 4( 3 ) : 229-239
[14] Levin B R,Lipaitch M.Bonhoeffer S,Population biology.evolution and infectious disease:convergence and synthesis[J].Science.1999.283: 806-809
[15] Domi A，Moan B. Cloning the vaccinie virus genome as a bacterial atrificial chromosome in Eacherichia coli and recovery of infectious virus in mammalian cello[J],Proc Natl Acad Sci U S A.2002. 99(19):12415-12420.
[16] Le Mercier P, Jacob Y,Tanner K, et al.A novel expression cassette of lyssaviws shows that the distantly related Mokola virus can rescue a defective rabies virus genome [J].J. Virol，2002, 76(4):2024-2027.
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