甘草粗提物对鲫的免疫调节作用

王文博; 方平; 林小涛; 夏磊; 齐长宝; 汪建国; 孙军

甘草粗提物对鲫的免疫调节作用

王文博^1,2,
方平³,
林小涛^1, ,,
夏磊³,
齐长宝³,
汪建国⁴,
孙军¹

暨南大学水生生物研究所, 广州 510632
2. 中国水产科学研究院南海水产研究所, 广州 510300
3. 中国水产科学研究院, 北京 100039
4. 中国科学院水生生物研究所, 武汉 430072

基金项目:

广东省科技计划项目(2006B20301026)

广东省自然科学基金项目(04010453)

中国博士后科学基金(第39批)

中央级公益性科研院所基本科研业务费专项基金项目(2007TS16)资助

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- 收稿日期: 2006-12-28
- 修回日期: 2007-05-15
- 发布日期: 2007-09-24

THE IMMUNOREGULATIVE EFFECTS OF LIQUORICE EXTRACT ON CRUCIAN

Institute of Hydrobiology, Jinan University, Guangzhou 510632;
2. South China Sea Fisheries Research Institute, the Chinese Academyof Fishery Sciences, Guangzhou 510300;
3. Chinese Academy of Fishery Sciences, Beijing 100039;
4. Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072

摘要

摘要: 本研究对中药甘草(GlycyrrhizauralensisFiseh)进行了粗提,将粗提物按0.5%和2%的质量分数制成药饵投喂鲫鱼(Carassiusauratus)后,于第14天、28天、35天、42天5、6天采样检测其免疫指标。结果显示,低剂量组试验鱼血清溶菌酶活性逐渐升高,高剂量组在35d达到最大值(205.5±28.8)U/mL,之后略有下降。低剂量组和高剂量组试验鱼血清杀菌活性均有所波动,波动范围分别为62.0%-73.1%和75.0%-83.3%,就各期而言,对照组、低剂量组和高剂量组试验鱼的血清杀菌活性依次升高。另外,在淋巴细胞计数方面,高剂量组仅在第42天采样时显著高于对照组(p0.05),在其他采样期,试验组与对照组均无统计学差异。此外,低剂量组试验鱼血液中白细胞吞噬活性有所增强(p0.05),第42天达到最大值(66.5±9.4)%,高剂量组先升后降,在第35天达到峰值(74.7±5.6)%。最后,低剂量组试验鱼脾脏重量指数在第56天显著高于对照组(p0.05),结果分别为(1.08±0.09)×10-3和(0.71±0.09)×10-3,高剂量组在第28天与对照组差异极显著(p0.01),结果分别为(1.12±0.13)×10-3和(0.63±0.08)×10-3。实验表明,该甘草粗提物对鲫免疫功能有良好的调节作用,具有广阔的应用前景。
- 甘草 /
- 粗提物 /
- 鲫 /
- 免疫力
Abstract: The problem of fish disease in both natural fisheries and in intensive cultivation systems is a major subject of scientific investigation1 Researchers try to solve this problem by all kinds of methods1 It is reported that many herbal medicines have very good characters to enhance the disease resistance of mammals1 However, these medicines are seldom used in aquaculture up to the present1 The aimof this study is to investigate whether there have immunoregulative effects of liquorice (Glycyrrhiza uralensis Fiseh)-one kind of traditional Chinese medicine on crucian(Carassius auratus) 1 In this study, liquorice was roughly extracted and then the extract was added in basal fish feed at the doses of 015% and 2% to raise crucian,which were subsequently sampled at day 14, 28, 35, 42 and 56 to examine their immune indexes, including serum lysozyme activity, serum bactericidal activity, lymphocyte count, phagocytic percent of leucocytes and spleen weight index, which are very typical immunological parameters referring not only to cellular immunity but also to humoral immunity1. The results exhibited that the serum lysozyme activity of low dose group increased gradually, and that of high dose group reached the maximum (205.5 ?28.8) U/ mL at day 35, then decreased slightly1 Serum bactericidal activityof both low and high dose groupsfluctuatedfrom 6210% to 7311% andfrom 75.0% to 83.3%, respectively1 At every sampling, serum bactericidal activity of control group, low dose group and high dose group rised in turn1 As to lymphocyte count, high dose group showed a higher value than control group only at day 42(p < 0105), but no statistical difference was found at other times1 Additionally, phagocytic percent of leucocytes of low dose group enhanced remarkably(p < 0105), and the maximum(6615 ?914)% ap2 peared at day 42, while that of high dose group increased, then decreased, with the peak value being(7417 ?516)% at day 351 Finally, spleen weight indexof low dose group was higher than that of control group at day 56(p < 0105), with the value being (1.08 ?0.09) × 10-3and(0.71 ?0.09) ×10-3,respectively1 Spleen weight index of high dose group was(1.12 ?0.13) × 10-3at day 28, higher than that of the control(0163 ?0108) ×10-31. As is known, teleost fish possess a variety of specific and nonspecific defence mechanisms against invading organisms1 When a pathogen penetrates the physical barriers of the animal, the first lines of defence it encounters are those of the nonspecific immune system1 Chemical defences attack the pathogen or mayopsonise it for further destruction by the cellular components of the nonspecific immune system1 Therefore, the nonspecific immune systemof fish is very important in resistance to disease1 The present study indicated that this kind of liquorice extract significantly improved some nonspecific immune indexes of crucian, such as lysozyme activity, bactericidal activity and phagocytic activity, showing its immunomodulatory effects on crucian and a promising future to apply in aquaculture1
- :Liquorice /
- Extract /
- Crucian /
- Immunity

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参考文献(18)

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甘草粗提物对鲫的免疫调节作用

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THE IMMUNOREGULATIVE EFFECTS OF LIQUORICE EXTRACT ON CRUCIAN

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