鲤和团头鲂幼鱼卵黄蛋白原的诱导、纯化及电泳比较

INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP(CYPRINUS CARPIO)AND WUCHANGFISH(MEGALOBRAMA AMBLYCEPHALA YIH)

摘要: 采用腹腔注射人工雌激素17α-乙炔基雌二醇(17α-ethinyloestradiol, EE2)的方法,在鲤幼鱼和团头鲂幼鱼体内诱导生成卵黄蛋白原;并结合利用阴离子交换介质 DEAE-Sephrarose CL-6B 和液相层析技术分离纯化了诱导后的鲤幼鱼和团头鲂幼鱼血浆中的卵黄蛋白原;SDS-PAGE 电泳结果表明所分离的两种鲤科鱼类卵黄蛋白原其亚基的分子量分别为170KD 和10KD.

Abstract: Vitellogenin was induced in immature common carp(Cyprinus carpio L.)and immature Wuchangfish(Megalobrama amblycephala Yih)by intraperitoneal injection of xenobiotic estrogen 17α-ethinyloestradiol. By using the anion-exchange chromatography on a DEAE-Sephrarose CL-6B column, the vitellogenin in plasma from two cyprinid fish species was effectively purified. The results of SDS-PAGE show that the molecular weights of vitellogenin monomer in two cyprinid fish species are around 170KD and 150KD respectively.