中国林蛙皮肤丝氨酸蛋白酶抑制剂RCSPI2的基因克隆和原核表达
CLONING AND PROKARYOTIC EXPRESSION OF THE SERINE PROTEASE INHIBITOR RCSPI2 FROM THE SKIN OF CHINESE BROWN FROGS, RANA CHENSINENSIS
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摘要: 丝氨酸蛋白酶抑制剂广泛存在于动物、植物及微生物体内[1], 是一类丝氨酸蛋白酶活性调节剂, 能调节生物体内许多重要的生命过程, 如蛋白质折叠、血凝、补体激活、炎症反应、细胞迁移、细胞基质重建、以及肿瘤抑制等[2,3], 很多已被开发为新药, 在临床上有广泛应用。基于其序列、拓扑结构及功能的相似性, 能分成16 个家族[4],是一个蛋白超家族, 每个家族成员都具有相似的氨基酸顺序、保守的三级结构和独特的作用机制。
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Keywords:
- Rana chensinensis /
- RCSPI2 /
- Serine protease inhibitor /
- Gene cloning
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