低温驯化下锯缘青蟹肝胰腺蛋白质表达及脂肪酸组成的变化
CHANGES OF PROTEIN EXPRESSION AND FATTY ACID COMPOSITION IN HEPATOPANCREAS OF SCYLLA SERRATA UNDER LOW TEMPERATURE ACCLIMATION
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摘要: 锯缘青蟹(Scylla serrata)在不同低温下经3周驯化后,采用双向电泳(Two division electrophoresis,2D-E)和气相色 谱(Gas chromatography,GC)分别对肝胰腺中蛋白质表达和脂肪酸组成变化进行研究,结果发现5℃驯化组与27℃组 相比,肝胰腺中共有22种蛋白的变化,其中5℃驯化组有15种蛋白在27℃组没有检出,而27℃组有7种蛋白在5℃ 驯化组没有发现;10℃驯化组与27℃组相比,共有18种蛋白变化,10℃驯化组有14种蛋白在27℃组没有检出,27℃ 组有4种蛋白在10℃驯化组没有出现;锯缘青蟹15℃驯化组与27℃组相比,肝胰腺中有6种蛋白质有变化,其中驯 化组分离出5种在27℃组没有检出的蛋白质,27℃组分离出1种在驯化组没有出现的蛋白质。27℃组没有检出,仅 在驯化组中出现的蛋白质中,经Imagemaster2D Elite软件分析后发现5℃和10℃驯化组有14种蛋白质在图谱上是匹 配的(即是同样的),15℃驯化组表达的5种蛋白与5℃驯化组和10℃驯化组表达的蛋白不匹配。低温驯化3周后 锯缘青蟹肝胰腺中饱和脂肪酸∑SFA在5℃和10℃驯化组显著低于27℃组(P>0．05),而∑UFA在5℃和10℃驯化 组显著高于27℃组(P>0．05),相应地5℃和10℃驯化组饱和指数∑SFA/∑UFA也显著低于27℃组(P>0．05),其 主要是∑PUFA-ω6低温驯化下显著升高(P>0．01)所致。结论表明锯缘青蟹不同低温驯化下,肝胰腺细胞中蛋白 质表达是一种动态变化模式。驯化温度越低,差异蛋白变化越大。低温驯化下脂肪酸饱和指数降低,主要是维持 低温下肝胰腺正常的生理功能。Abstract: Mud crab, Scylla serrata , is an economic crustacean living in estuarine waters. Studies on mechanism of low temperature adaptation are important for low temperature breeding,as well as crab aquiculture. Two-division electrophoresis, including isoelec-trophoresis focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ,was important method for proteins division in proteomes, which was used to determine expression of specific proteins in hepatopancreas of Scylla serrata under low temperature acclimation. In addition, gas chromatography was used to determine fatty acid composition in different low temperature acclimation groups. Scylla serrata , were enforced to experience 3 weeks acclimation under different low temperature, hepatopancreas of whom was taken out from each crab respectively. Hepatopancreas from ten crabs of each group was mixed to perform experiment. The experiment results showed that there were 22 difference proteins in hepatopancreas between 5 ℃ acclimation group and 27℃ group. 15 proteins were expressed in 5℃ acclimation group and without in 27℃ group .there were 7 proteins in 27℃ group and without in 5℃ acclimation group, there were 18 difference proteins between 10℃ acclimation group and 27℃ group,namely that, 14 specific proteins were expressed in 10℃ acclimation group,and 4 in 21℃ group;there were 6 specific difference proteins between 15℃ acclimation group and 27℃ group, 5 specific proteins were expressed in 15℃ acclimation group and without in 27℃ group,one protein appeared in 27℃ group and without in 15℃ acclimation group. For matches of difference proteins in different acclimation groups, 14 proteins were matched in 5℃ and 10℃ group, while specific difference proteins in 15℃ group were not matched with 5℃ and 10℃ at all. these results indicated that specific proteins expressed in 5℃ and 10℃ were almost corresponding, while those expressed in 15℃ group is resmble with 27℃ group. It is obvious that difference proteins between acclimation group and 27℃ group increased with acclimation temperature decrease. After acclimation of 3 weeks, the sum of the saturated fatty acids (∑SFA)in hepatopancreas in 5℃ and 10℃ acclimation groups were significantly lower than that in 27℃ group (PPPP<0.01)under low temperature acclimation. During the process of low temperature acclimation, changes in protein expression are dynamic. The lower acclimation temperature it is,the more specific difference proteins are expressed, the saturated index of fatty acid (∑SFA/∑UFA) decreases under low temperature acclimation. Changes of protein expression and fatty acid composition in low temperature are used to adapt to environmental changes and to maintain the physiological function of the hepatopancreas.
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Keywords:
- Difference proteins /
- Fatty acid /
- Hepatopancreas /
- Low temperature acclimation /
- Scylla serrata
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