Abstract: This paper presents a survey of lactate dehydrogenase isozyme patterus of 14 species and 4 hybrids of Cyprinidae. Various tissues were analyzed by polyacrylamide gel electrophoresis, including muscle, heart, lens, brain and kidney. The results of the experiments are summarized as follows:The LDH patterns of fishes of the same subfamily may be remarkably different. Based on the difference of LDH isozyme patterns, we can divide 5 species of Leuci scinae into 2 groups: (1) Ctenopharygodon idellus, Elopichthys bambusa, and Ocheto bius elongatus, (2) Mylopharyngodon piceus and Squaliobarbus curriculus. In Abramidinae, the LDH isozyme pattern of Erythroculter dabryi is similar to that of Parabramis pekinensis, Megalobrama amblycephala and Cultcr erythroptcrus; but is strikingly different from that of Hemiculter leucisculus. In hypophthalmichthyinae, the LDH isozyme pattern of Aristichthys nobilis is similar to that of Hypophthalmichthys molitrix. In Xenocyprinae, the LDH isozyme pattern of Plagiognathops microlepis and that of Xenocypris davidi appear quite similar, too. Based on the LDH isozyme patterns, the phylogenetic relationship of the 14 species of Cyprinidae can be demonstrated to some extent.