草鱼、中华鳖T淋巴细胞表面抗原的研究
STUDIES ON SURFACE ANTIGENS OF T LYMPHOCYTE IN GRASS CARP,CTENOPHARYNGODON IDELLUS AND CHINESE SOFT SHELLED TURTLE,TRIONYX SINENSIS
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摘要: 以草鱼脑组织、中华鳖脑组织和胸腺细胞为抗原制备兔抗草鱼脑血清(RACBS)、兔抗中华鳖脑血清(RATBS)和兔抗中华鳖胸腺细胞血清(RATTS).补体依赖性细胞毒试验和不同组织对RATBS、RATTS的吸收试验结果表明:中华鳖胸腺细胞和脑组织均存在Thy1抗原(亦称脑组织抗原或胸腺-脑组织T细胞抗原);草鱼脑组织缺乏Thy1抗原.应用间接酶标免疫组化染色技术显示:Thy1抗原阳性反应物沉淀于中华鳖胸腺细胞和外周一部分淋巴细胞表面.进一步用抗人白细胞分化抗原CD4、CD8单克隆抗体进行免疫组化交叉反应提示中华鳖淋巴细胞膜上含有与人类T淋巴细胞表面抗原CD4、CD8类似结构的物质.本文讨论了Thy1抗原、CD抗原的出现及其意义,探讨了淋巴细胞异质性及RATBS、RATTS的特异性和作用.Abstract: Rabbit anti grass carp(Ctenopharyngodon idellus)brain serum(RACBS),rabbit anti Chinese soft shelled turtle(Trionyx sinensis)brain serum(RATBS)and rabbit anti Chinese soft shelled turtle(Trionyx sinensis)thymocyte serum(RATTS)were obtained. By complement dependent cytotoxicity assay and respective absorption assay of RATBS,RATTS with thymocyte,brain tissus of Chinese soft shelled turtle and grass carp, it is shown that there are Thy1 antigens(a brain associated thymus antigen or thymus brain antigen)on thymocytes and brain of Chinese soft shelled turtle,but absent in brain of grass carp. By indirect immuno peroxidase histochemistry staining,it is shown that the Thy1 antigens appear on the membrane of thymocytes and a parts of peripheral lymphocytes. Further,with indirect immunohistochemistry technique,CD4,CD8 cross reactions have been identified on lymphocyte membrane of Chinese soft shelled turtle using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD4,CD8. In this paper,the persence and the significance of Thy1 antigen and CD,the specificity and the action of RACBS and RATTS,the hetergeneity of lymphocytes have been discussed.
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