日粮必需氨基酸模式对草鱼生长及蛋白质周转的影响
EFFECT OF DIETARY ESSENTIAL AMINO ACID PATTERN OF THE GROWTH AND PROTEIN TURNOVER OF GRASS CARP (CTENOPHARYNGODON IDELLUS)
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摘要: 通过调整原料种类和配比,以及补充氨基酸,设计了6种必需氨基酸(EAA)模式的日粮,其EAA平衡关联度分别为0.7071,0.729,0.7409,0.712,0.7827和0.8231.试验设计目的为研究EAA模式的平衡效果对草鱼种生长和肌肉、肝胰脏蛋白质周转代谢的影响.结果表明:(1)日粮EAA模式的平衡,促进草鱼生长和饲料的转化;(2)EAA模式的平衡能提高肌肉、肝胰脏的蛋白质生长速率(FGR),和蛋白质合成速率(FSR)与降解速率(FDR),但EAA模式的改变,对肌肉、肝胰脏蛋白质的沉积效率(PRE),即FGR与FSR的比值,和蛋白质合成的翻译效率(KRNA)不产生影响.(3)日粮EAA模式的平衡促进肌肉、肝胰脏蛋白质增长的原因是蛋白质合成能力的提高,和蛋白质合成的增加较降解的增加更占优势.Abstract: By changing type and proportion of ingredients and supplying amino acids, six dietary EAA patterns are designed, in which Dietary Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ and Ⅵ respectively are in 0.7071,0.7259,0.7409,0.7512,0.7827 and 0.8231 relativity of EAA equilibrium. The purpose of the experimental design is to study effects of dietary EAA balance on the growth and protein synthesis and degradation in muscle and hepatopancreas of grass carp. Results show that: 1. The balanced EAA pattern enforce growth of grass carp and transformation of feeds. 2. The balanced EAA pattern can increase protein synthesis and degradation rate (FSR, FDR) and fractional protein growth rate (FGR) of muscular organism and hepatopancreas, but following the change of EAA pattern in diets, protein retention efficiency (PRE), that is FSR/FDR, and translational efficiency of protein synthesis (KRNA) didn't show significant diference. 3. For the muscular orginism, relationships between FGR?FSR? FDR and relativity of EAA equilibrium is: YFGR=3.7487x-1.3991 (r=0.9445), YFSR=18.734x-7.4771 (r=0.9597), YFDR=14.985x- 6.078 (r=0.9625), respectively. 4. For the hepatopancreas, relationships between FGR、FSR、FDR and relativity of EAA equilibrium is: YFGR =4.2788x-1.6545 (r=0.9399),YFSR=43.262x-16.207 (r=0.9315),YFDR=38.983x-14.553 (r= 0.9303) respectively. 5. In the six dietary EAA patterns, FSR and FDR in the hepatopancreas are remarkably higher than in the muscular orginism. 6. The balanced EAA pattern can stimulate a positive protein growth, partially attributing to increased protein synthesis capacity, greater difference between synthesis and degradation.
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Keywords:
- Protein turnover /
- EAA pattern /
- muscle /
- Hepatopancreas /
- Ctenopharyngodon idellus
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[1] Liu S M. Method of measuring protein turnover and relationship between nutrition and turnover of ruminant[A]. Advance on study of animal nutrition[C]. Beijing: China Agricultural University Press,1996.201-222.[刘世民.反刍动物蛋白质周转的测定方法和营养与周转的关系.动物营养研究进展.北京:中国农业大学出版社,1996.201-222][2] Ye Y T. Effects of EAA balanced state in diets on growth of grass carp (Ctenopharyngodon idellus) [J]. Feed industry, 1998,20(3):97-103.[叶元土.饲料必需氨基酸的平衡效果对草鱼生长的影响.饲料工业,1998,20(3):90-103][3] Zeng D. Effect of EAA balanced pattern individual AA on juvenile grass carp (Ctenopharyngodon idellus) intestinal absorption of Leu and Phe[D]. Master degree dissertation, 1999.[曾端. EAA 平衡模式及个别 AA 对草鱼幼鱼肠道吸收 Leu、Phe 速率的影响[D].硕士学位论文,1999][4] Jiangshang Xingxiong. Experimental animal of fish[M].Beijing: Ocean Press, 1992.55-56.[江上信雄.鱼类实验动物[M].北京:海洋出版社,1992.55-56][5] Langer H. Augmentation of protein sysnthesis and degration by poor dietary amino acid balance in European sea bass[J]. J.Nutr., 1993,123(10):1754-1761.[6] Luo L, Ye Y T, lin S M. Effects of EAA pattern solution on protein synthesis in whole body, muscle and heapatopancreas of Ctenopharyngodon idellus [J]. J Fish china. 2002,26(1):75-78.[罗莉,叶元土,林仕梅.灌喂 EAA 模式溶液对草鱼肌肉、肝胰脏蛋白质合成代谢的影响.水产学报,2002,26(1):75-78][7] Li Y. Study on the changes of serum calcium ion, phosphorous compounds and liver nucleic acid in Pelteobagrus vachelli during reproductive cycle[D]. Master degree dissertation, 1999.[李云.生殖周期瓦氏黄颡鱼血清钙离子和磷化合物及肝脏核酸的变化研究.硕士学位论文,1999][8] Ye Y T, Lin S M, Luo L. Apparent digestibility coefficient of amino acids in 27 feed ingredients for grass carp (Ctenopharyngodon idellus)[J]. Fisher sci. china. 2003,10(1):60-64.[叶元土,林仕梅,罗莉.草鱼对27种饲料原料的氨基酸表观消化率.中国水产科学,2003,10(1):60-64][9] Houlihand D F. Protein synthesis in a fish heart: respondes to increased power output[J]. J Biol Chem, 1988,246:436-446[10] Tesseraud S, Maaa N, peresson R, et al. Relative responses of protein turnover in three different skeletal muscles to dietary lysine deficiency in chicks[J]. Bri Poult Sci, 1996,37(3): 641-650[11] Tesseraud S. Protein metabolism in the growing fowl. Effect of dietary proteins[J]. Production Animals, 1995,8(3): 197-212[12] Zhou A G, Qi L G, Liu Y Q. Study of nutritional ang physiological effect of whole-body protein turnover in ducklings at early stages of growth[J]. Acta Veterinaria Zootechnica Sinica, 1995,6(2): 97-103.[周安国,漆良国,刘永前. 生长肉鸭体蛋白周转的营养生理效应研究.畜牧兽医学报,1995,6(2):97-103] Liu S M. Method of measuring protein turnover and relationship between nutrition and turnover of ruminant[A]. Advance on study of animal nutrition[C]. Beijing: China Agricultural University Press,1996.201-222.[刘世民.反刍动物蛋白质周转的测定方法和营养与周转的关系.动物营养研究进展.北京:中国农业大学出版社,1996.201-222][2] Ye Y T. Effects of EAA balanced state in diets on growth of grass carp (Ctenopharyngodon idellus) [J]. Feed industry, 1998,20(3):97-103.[叶元土.饲料必需氨基酸的平衡效果对草鱼生长的影响.饲料工业,1998,20(3):90-103][3] Zeng D. Effect of EAA balanced pattern individual AA on juvenile grass carp (Ctenopharyngodon idellus) intestinal absorption of Leu and Phe[D]. Master degree dissertation, 1999.[曾端. EAA 平衡模式及个别 AA 对草鱼幼鱼肠道吸收 Leu、Phe 速率的影响[D].硕士学位论文,1999][4] Jiangshang Xingxiong. Experimental animal of fish[M].Beijing: Ocean Press, 1992.55-56.[江上信雄.鱼类实验动物[M].北京:海洋出版社,1992.55-56][5] Langer H. Augmentation of protein sysnthesis and degration by poor dietary amino acid balance in European sea bass[J]. J.Nutr., 1993,123(10):1754-1761.[6] Luo L, Ye Y T, lin S M. Effects of EAA pattern solution on protein synthesis in whole body, muscle and heapatopancreas of Ctenopharyngodon idellus [J]. J Fish china. 2002,26(1):75-78.[罗莉,叶元土,林仕梅.灌喂 EAA 模式溶液对草鱼肌肉、肝胰脏蛋白质合成代谢的影响.水产学报,2002,26(1):75-78][7] Li Y. Study on the changes of serum calcium ion, phosphorous compounds and liver nucleic acid in Pelteobagrus vachelli during reproductive cycle[D]. Master degree dissertation, 1999.[李云.生殖周期瓦氏黄颡鱼血清钙离子和磷化合物及肝脏核酸的变化研究.硕士学位论文,1999][8] Ye Y T, Lin S M, Luo L. Apparent digestibility coefficient of amino acids in 27 feed ingredients for grass carp (Ctenopharyngodon idellus)[J]. Fisher sci. china. 2003,10(1):60-64.[叶元土,林仕梅,罗莉.草鱼对27种饲料原料的氨基酸表观消化率.中国水产科学,2003,10(1):60-64][9] Houlihand D F. Protein synthesis in a fish heart: respondes to increased power output[J]. J Biol Chem, 1988,246:436-446[10] Tesseraud S, Maaa N, peresson R, et al. Relative responses of protein turnover in three different skeletal muscles to dietary lysine deficiency in chicks[J]. Bri Poult Sci, 1996,37(3): 641-650[11] Tesseraud S. Protein metabolism in the growing fowl. Effect of dietary proteins[J]. Production Animals, 1995,8(3): 197-212[12] Zhou A G, Qi L G, Liu Y Q. Study of nutritional ang physiological effect of whole-body protein turnover in ducklings at early stages of growth[J]. Acta Veterinaria Zootechnica Sinica, 1995,6(2): 97-103.[周安国,漆良国,刘永前. 生长肉鸭体蛋白周转的营养生理效应研究.畜牧兽医学报,1995,6(2):97-103]
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