团头鲂线粒体DNA的限制性内切酶图谱

A MAP OF RESTRICTION ENDONUCLEASES OF MEGELOBRAMA AMBLYCEPHALA YIH

摘要: 用BamHⅠ,BglⅠ,BglⅡ,EcoRⅠ,HpaⅠ,KpnⅠ,PstⅠ,SacⅠSalⅠ,XbaⅠ,和XhoⅠ11种限制性内切酶对团头鲂(Megalobrama amblycephala Yih)的线粒体DNA(mtDNA)进行了单酶切,其切点数依次为2,3,2,3,3,1、0,1,0,0和0;经琼脂糖凝胶电泳测算出各酶切片断大小,得出团头鲂mtDNA分子长约16020±356碱基对(bp),分子量6.37×1018u(原子质量),构建了团头鲂mtDNA的限制酶图。

Abstract: The mitochondrial DNA (mtDNA) of Megelobrama amblycephala were digested by 11 restriction endonucleases: BamH Ⅰ, Bgl Ⅰ, Bgl Ⅱ, EcoR Ⅰ, Hpa Ⅰ, Kpn Ⅰ, Pst Ⅰ, Sac Ⅰ,Sal Ⅰ, Xba Ⅰ, Xho Ⅰ into different fragment: 2, 3, 2, 3, 3, 1, 0, 1, 0, 0, 0 respectively. Estimated by agarose gel electrophoresis, the size of mtDNA from Megelobrama amblycephala is 16020 ±356 bp. When comparing the double restriction fragments with standard molecular weight of Mark Ⅰ (Hind Ⅲ digested λDNA) and Mark Ⅱ(EcoR Ⅰand Hind Ⅲ digested λDNA), we constructed the enzymes physical map.