满江红鱼腥藻遗传多样性的RAPD分析
THE GENETIC DIVERSITY OF ANABAENA AZOLLAE BASED ON RAPD ANALYSIS
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摘要: 共生在水生蕨类植物满江红(又称“红萍”:Azolla)叶腔中的固氮兰细菌曾被认为是一个种满江红鱼腥藻(Anabaena azollae Strasb.).但由于现存的满江红在分类学上有2个亚属,7个种[1],对不同满江红叶腔中鱼腥藻的鉴别,多年来引起人们的重视.80年代,鱼腥藻的单克隆抗体和RFLP的研究,发现了它与宿主分类上一定程度的对应关系[2,3].90年代以后,Van Coppenolle和Eskew分别以RAPD-PCR和DAF-PCR对从满江红共生体中提取的DNA进行了分析[4,5],但后者发现了共生体中藻的DNA对整个PCR产物的干扰作用会影响满江红属本身系统分类.
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Keywords:
- Anabaena azollae /
- Azolla /
- Genetic diversity /
- RAPD analysis
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[1] Lumpkin T A, Plucknett D L. Azolla: botany, physiology and use as green manure[J]. Econ Bot, 1980, 34: 111-153[2] 刘中柱等. 满江红鱼腥藻单克隆抗体的制备和研究应用[J]. 中国科学B辑,1989,(1): 44-52[3] Franche C, Cohen-Bazire G. Evolutionary divergence in the nif H.D.K gene region among nine symbiotic Anabeana azollae and between heterocystus cyanobacteria[J]. Symbiosis, 1987, 3: 159-178[4] Van Coppenolle B. Genetic diversity and phylogeny analysis of Azolla based on DNA amplification by arbitrary primers[J]. Genome, 1993, 36: 686-693[5] Eskew D L. DNA amplification fingerprinting of the Azolla-Anabeana symbiosis[J]. Plant Mol Biology, 1993, 21: 363-373[6] Van Coppenolle B. Genetic diversity and phylogeny analysis of Anabeana azollae based on RFLPs detected in Azolla-Anabeana azollae DNA complex using nif probes[J]. Theor Appl Genet, 1995, 91: 589-597[7] Zheng W W. Genetic diversity and classification of cyanobacteria in different Azolla species by the use of PCR fingerprinting[J]. Theor Appl Genet, 1999, 99: 1187-1193[8] Caudales R. Fatty acid composition of symbiotic cyanobacteria from different host plant(Azolla)species: evidence for coevolution of host and symbiont[J]. Int J Syst Bacteriology, 1995, 45(2): 364-370[9] 白克智. 无藻满江红(Azolla)和满江红鱼腥藻(Anabeana azollae)的分离与培养[J]. 科学通报,1979,(14): 664-666 Lumpkin T A, Plucknett D L. Azolla: botany, physiology and use as green manure[J]. Econ Bot, 1980, 34: 111-153[2] 刘中柱等. 满江红鱼腥藻单克隆抗体的制备和研究应用[J]. 中国科学B辑,1989,(1): 44-52[3] Franche C, Cohen-Bazire G. Evolutionary divergence in the nif H.D.K gene region among nine symbiotic Anabeana azollae and between heterocystus cyanobacteria[J]. Symbiosis, 1987, 3: 159-178[4] Van Coppenolle B. Genetic diversity and phylogeny analysis of Azolla based on DNA amplification by arbitrary primers[J]. Genome, 1993, 36: 686-693[5] Eskew D L. DNA amplification fingerprinting of the Azolla-Anabeana symbiosis[J]. Plant Mol Biology, 1993, 21: 363-373[6] Van Coppenolle B. Genetic diversity and phylogeny analysis of Anabeana azollae based on RFLPs detected in Azolla-Anabeana azollae DNA complex using nif probes[J]. Theor Appl Genet, 1995, 91: 589-597[7] Zheng W W. Genetic diversity and classification of cyanobacteria in different Azolla species by the use of PCR fingerprinting[J]. Theor Appl Genet, 1999, 99: 1187-1193[8] Caudales R. Fatty acid composition of symbiotic cyanobacteria from different host plant(Azolla)species: evidence for coevolution of host and symbiont[J]. Int J Syst Bacteriology, 1995, 45(2): 364-370[9] 白克智. 无藻满江红(Azolla)和满江红鱼腥藻(Anabeana azollae)的分离与培养[J]. 科学通报,1979,(14): 664-666
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