斗嫁卵子发生和雌性性腺组织学研究
THE OOGENESIS AND FEMALE GONADAL HISTOLOGY OF CELLANA GRATA(GOULD)
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摘要: 通过组织切片和活细胞涂片对斗嫁Cellana grata(Gould)性腺和生殖细胞进行观察,研究雌性性腺特征及不同发育阶段雌性生殖细胞的形态特点与分布状况。观察与分析表明:(1)斗嫁雌性滤泡由卵原细胞及不同发育时期的初级卵母细胞填充,成熟的初级卵母细胞位居滤泡腔中央区域;(2)性细胞发育不同步,属分批产卵类型;(3)卵子发生中,细胞、核及核仁大小变化的总体趋势是随着发育进程而逐渐增大,常见1-2个核仁;(4)卵质的酸碱特性变化与卵黄的出现相关;(5)成熟卵母细胞大小约为(120-140)μm×150μm,外被约5-12μm厚的胶质膜;(6)胶质膜的均一性、厚度、酸碱性随卵发育而变化,胶质膜与卵膜之间出现间隙可以作为卵开始成熟的标志,而胶质膜由嗜酸性转化为嗜碱性可以作为卵成熟即将排放的标志。Abstract: The gonad and gametocyte of Cellana grata (Gould) were observed under microscope by puncture and historical section to study the characteristic of female gonad and the morphology and distribution of oogametes at different developing stages. The results showed that: (1) the female folliculus of C. grata were composed of oogoniums and oocytes at different developing stages, and matured oogametes were located around the central region of follicle; (2) The development of gametocytes was asynchronous and spawning came up on successive type. (3) The gametocytes and its nuclei and nucleolus trended to enlarge during the oogenesis with the maturity of the gonad, and 1-2 nucleolus appeared. (4) Characteristic of acido-and baso-philic of oocytoplasma changed with coming up of oocytoyolk. (5) Matured egg were in range of about (120-140) μm × 150 μm in size, which were rounded by an jelly coat with thickness 5-12 μm. (6) The uniformity and acidity of jelly coat were changeable according to the developing stages of gametocyte. It was the signal of oogoniums becoming mature that gaps appeared between jelly coat and the oocyte membrane; and it was another signal of oogoniums being matured and went to spawn that acidophilic of the oogoniums transformed to basophilic.
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Keywords:
- Cellana grata (Gould) /
- Oogenesis /
- Female gonad /
- Jelly coat
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[1] Cai Y Y,Xie S H.Seashells of Guangdong[M].Shantou:Shantou University Press.2006,59-64[蔡英亚,谢绍河.广东的海贝.汕头:汕头大学出版社.2006,59-64]
[2] Carlton J T,et al.The first historical extinction of a marine invertebrate in an ocean basin:The demise of the eelgrass limpet Lottia alveus[J].The Biological Bulletin,1991,180(1):72-80
[3] Jiang N Q,Shen J,Xu R M,et al.Studies on population ecology of the limpet Nacella concinna in the intertidal zone of fildes Peninsula,Antarctic-Spatial Distribution Patterns[J].Oceanologia Et Limnologla Sinica,2000,31(5):511-517[蒋南青,沈静,徐汝梅,等.南极菲尔德斯半岛潮间带南极帽贝的种群生态学研究-空间分布图式.海洋与湖沼,2000,31(5):511-517]
[4] Tang S M.Comparisons of population structure of Antarctic limpet(Nacella concinna)on the east and west coasts of southern fildes Peninsula[J].Chinese Journal of Polar Research,2006,18(3):197-205[唐森铭.南极菲尔德斯半岛两侧南极帽贝种群结构比较分析.极地研究,2006,18(3):197-205]
[5] Dawn K P,Paul A T,Lloyd S P.Effect of sperm concentration and sperm aging on fertilization success in the Antarctic soft-shelled clam Latemula elliptica and the Antarctic limpet Nacella concinna[J].Marine Ecology Progress Series,2001,215:191-200
[6] Robertson R F,El-haj A J,Clarke A,et al.The effects of temporature on metabolic rate and prote in synthesis following a meal in the isopod Glyptonotus antarcticus Eights[J].Polar Biology,2001,24(9):677-686
[7] de Moreno J E A,Gerpe M S,Moreno V J,et al.Heavy metals in Antarctic organisms[J].Polar Biology,1997,17(2):131-140
[8] Ahn I Y,Kyung H o Chung,Heesenn J Choi.Influence of glacial runoff on baseline metal accumulation in the Antarctic limpet Nacella concinna from King George Island[J].Marine Pollution Bulletin,2004,49(12):119-127
[9] McCarthy M,Woosnam P,Culloty S C.Histological investigation of the reproductive cycles of the limpets Patella vulgata and Patella ulyssiponensis[J].Marine Biology,2008,153(5):871-877
[10] Hodgson A N,Le Quesne W J F,Hawkins S J,et al.Factors affecting fertilization success in two species of patellid limpet(Mollusca:Gastropoda)and development of fertilizationkinetics models[J].Marine Biology,2007,150:415-426
[11] John B N,Brent H.Spermiogenesis and sperm structure in relation to early events of fertilization in the limpet Tectura testudinalis(Muller,1776)[J].The Biological Bulletin,1997,193:306-319
[12] Wang Z Z,Wu C W,Liu Z Y.Preliminary study on the ecologicals,habits and characteristics of Cellana toreuma(Reeva)in the north of Zhejiang Coastal Area[J].Journal of Zhejiang Ocean University(Natural Science),2001,20(1):7-13[王志铮,吴常文,刘祖毅.浙江北部沿海嫁()Cellana toreuma(Reeve)生态习性的初步研究.浙江海洋学院学报,2001,20(1):7-13]
[13] Wang Y N,Zhou Y F,Ding L F,et al.Mollusca resource in the sea shore of Inter-tide Zone,Wenlin City Shitang Town,Zhejiang Province[J].Journal of Ningbo University,2003,16(1):40-43[王一农,周友富,丁理法,等.浙江温岭石塘沿海岛屿的潮间带贝类资源.宁波大学学报,2003,16(1):40-43]
[14] Liang C Y,Zhang H H,Wu J F,et al.Main animals of economic value in Rocky zone of Guiling Island[J].Journal of Zhanjiang Ocean University,2000,20(3):71-74[梁超愉,张汉华,吴进锋,等.龟龄岛礁滩的主要经济动物.湛江海洋大学学报,2000,20(3):71-74]
[15] Wang Z Z,Wu C W.Study on the age and growth of Cellana toreuma(Reeva)in the north of Zhejiang Coastal Area[J].Journal of Zhejiang Ocean University(Natural Science),2000,19(4):316-323,326[王志铮,吴常文.浙北沿海嫁()Cellana toreuma(Reeve)年龄与生长的研究.浙江海洋学院学报,2000,19(4):316-323,326]
[16] Yu H W,Li T W,Su X R,et al.Histological observation on the testis in Cellana toreuma(Reeve)[J].Fisheries Science,2004,23(3):13-15[余红卫,李太武,苏秀榕,等.嫁(3)精巢的组织学研究.水产科学,2004,23(3):13-15]
[17] Zhang Y P.An analysis on nutritive compositions in meat of Cellana toreuma[J].Journal of Zhejiang Ocean University,2002,21(1):38-40,50[张永普.嫁(1)肉营养成分的分析.浙江海洋学院学报,2002,21(1):38-40,50]
[18] Wang X A,Fu G F.The microscopical structure of the center nervous ganglia of Cellana toreuma[J].Chinese Journal of Zoology,1998,33(4):11-13[王晓安,傅更锋.嫁(4)螺神经系统结构的初步研究.动物学杂志,1998,33(4):11-13]
[19] Shen Y P,Ma L J,Zhang X Y,et al.Gametogenesis of Pinctada martensii Dunker[J].Acta Zoologica Sinica,1992,38(2):113-116[沈亦平,马丽君,张锡元,等.合浦珠母贝的配子发生.动物学报,1992,38(2):113-116]
[20] Wang M F,Yu X Y,Wang R C.Gametogenesis in Atria pectinata Linnaens(Mollusca,Bivalvia)[J].Journal of Ocean University of Qingdao(Natural Science),2000,30(3):441-446[王梅芳,余祥勇,王如才.栉江珧生殖细胞的发生.青岛海洋大学学报(自然科学版),2000,30(3):441-446]
[21] Lin Z H,Shan L Z,Chai X L,et al.Study on the gonad develolnnent and reproductive cycle of Meretrix meretrix Linnaeus[J].Journal of Fisheries of China,2004,28(5):510-514[林志华,单乐州,柴雪良,等.文蛤的性腺发育和生殖周期.水产学报,2004,28(5):510-514]
[22] Ying X P.Study on the ultrastructural of oogenesis of Bullacta exarata[J].Developmental & Reproductive Biology,2002,11(1):29-36[应雪萍.泥螺卵子发生的超微结构研究.发育与生殖生物学报,2002,11(1):29-36]
[23] Zhou Y C,Chen G H,Su Y J.Anatomy and histology of the reprodustive system of Cymatium pileare[J].Journal of Fisheries of China,2000,24(5):393-398[周永灿,陈国华,苏永金.毛嵌线螺生殖系统的解剖学和组织学研究.水产学报,2000,24(5):393-398]
[24] Liu Y F,Liu Y X,Sui X L,et al.A study on reproductive cycle of disk abalone(Haliotis discus hainnai ino.)in Da Lian Coast[J].Journal of Fisheries of China,1985,9(4):311-319[刘永峰,刘永襄,隋锡林,等.大连海区皱纹盘鲍生殖周期的研究.水产学报,1985,9(4):311-319.]
[25] Sun Z X,Wang R C,Jiang M,et al.Morphology and ultrastructure of egg of Haliotis discus hannai[J].Journal of Fishery Sciences of China,1997,4(1):68-74[孙振兴,王如才,姜明,等.皱纹盘鲍卵的形态与超显微结构.中国水产科学,1997,4(1):68-74]
[26] Lou Y D.Histology and Embryology[M].Beijing:Agriculture Press.1999,239-247[楼允东.组织胚胎学.北京:农业出版社.1999,239-247]
[27] Wang M F,Yu X Y,Liu Y,et al.Study on hermaphrodites and self-fertilization of the pearl oyster pinctada martensii[J].Acta Hydrobiologica Sinica,2006,30(4):420-424[王梅芳,余祥勇,刘永,等.马氏珠母贝雌雄同体和自体受精的研究.水生生物学报,2006,30(4):420-424]
[28] Yang Y C,Li F X.The study on the reproductive cycle of Hiatulaacuta[J].Tropic Oceanology,1994,13(2):61-67[杨耀聪,李复雪.尖紫蛤生殖J占j期的研究.热带海洋,1994,13(2):61-67]
[29] Wang M F,Yu X Y,Ye F L.The gonad development of Pinna(Atrina)pectinata in Beibu gulf and adjoining coast[J].Guangxi Sciences,2000,7(2):140-143,157[王梅芳,余祥勇,叶富良.北部湾及附近海域栉江珧性腺发育研究.广西科学,2000,7(2):140-143,157]
[30] Wang M F,Cao X Y,Yu F F,et al.The spermatogensis and male gonadal histology of Cellana grata(Gould)[J].Acta Oceanologica Sinica,2009,31(6):142-150[王梅芳,曹新云,于菲菲,等.斗嫁(虫戚)精子发生及雄性性腺组织学研究.海洋学报,2009,31(6):142-150]
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