适合大量培养的红球藻藻种的筛选
SELECTION OF HAEMATOCOCCUS STRAINS SUITABLE FOR MASS CULTURE
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摘要: 研究了4个雨生红球藻(Haematococcus pluvialis)品系在10℃、1℃、20℃、2℃和28℃时的生长速率(μ)、生物量、虾青素含量和产量,从中选出适合于大量培养的品系H.pluvialis26和H.pluvialis WZ.2个品系的虾青素含量和产量分别达到2.41%-3.16%,46.1-4.3mg/L和2.40%-2.9%,39.84-40.1mg/L.2个品系的温度适应具有互补性:H.pluvalisWZ适应于较低温度(10℃-20℃),H.pluvialis26适应于较高温度(20℃-28℃),在低温季节使用H.pluvialis WZ,在高温季节使用H.pluvialis 26进行生产,可以延长生产期,提高产量.Abstract: Haematococcus pluvialis,a kind of unicellular freshwater green algae,has been proved to be one of the most effective natural sources of astaxanthin.Astaxanthin can be used in aquculture,food,medical and cosmetical industries etc,thus it has a promising future,due to its anti oxidation and effective coloration. Aim at the key technological problems for mass culture of the Haematococcus,4 strains of Haematococcus plurialis from our laboratory collection were studied.They were cultured under 10℃,15℃,20℃,25℃,28℃respectively,and were investigated growth rate,biomass,astaxanthin content and productivity.The results are as follow:1):Optimal cultivation temperature of 4 strains were H.pluvalis 26:20 ℃ ;H.pluvalis 30:25 ℃ ;H.pluvalis 34:20 ℃ ;H.pluvalis WZ:15℃respectively.2) Astaxanthin content and productivity of 4 strains were H.pluvalis 26:2.41%—3 16℃ and 46.51—54.35mg/L; H.pluvalis 30:0.94%—1.34% and 7.89—13.13mg/L; H.pluvalis 34:1.41%—1.83% and 10.53 —12 26 mg/L; H.pluvalis WZ: 2.40%—2.59% and 37.84—40.15mg/L.According to culture temperature,astaxanthin content and productivity, H.pluvialis 26 and H.pluvialis WZ were selected as strains suitable for mass culture. The temperature adaptation of H.pluvialis WZ and H.pluvialis 26 are complementary: H.pluvialis WZ is adaptable to lower temperature(10℃—15℃),and H.pluvialis26 is adaptable to higher temperature (20℃—28℃). Using H.pluvialis WZ in cool season and H.pluvialis 26 in warm season respectively for mass culture would extend production duration and thus realize higher productivity.
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Keywords:
- Haematococcus pluvialis /
- Astaxanthin /
- Mass culture /
- Strain selection
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[1] Grung M,D'Souza F M L,Borowitzka M,et al.Algal carotenoids 51.Secondary carotenoids 2.Haematocuccus pluvialis aplanospores as a source of (3S,3′S)-astaxanthin esters[J].J Appl Phycol,1992,4:165-171[2] Lee Y K,Soh C W.Accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta)[J].J Phycol,1991,27:575-577[3] Sommer T R,Potts W T.Utilization of microalgal astaxanthin by rainbow trout (oncorhynchus mykiss)[J].Aquaculture 1991,94:79-88[4] Kranzfelder J A.Carotenoids from microalgae[J].J Phycol,1992,28(suppl.):11[5] Wang Y Q,Li Q S,The studying progress,production and application of natural carotenoids[M]Beijing;China Medical pharmacy and Science Publishing house 1997[王业勤,李勤生.天然类胡萝卜素--研究进展、生产、应用.北京:中国医药科技出版社,1997][6] Terao J.Antioxidant activity of β-carotene-related carotenoids in solution[J].Lipids 1989,24:659-661[7] Miki W.Biological functions and activities of animal carotenoids[J].Pure Appl.Chem, 1991,63:141-146[8] Shimidzu N.Carotenoids as single oxygen quenchers in marine organisms [J].Fisheries Science 1996,62:134-137[9] Zhuang H R,Shi Qr Q,Lu H S,et al.The effect of nutritional stresses on accumulation of astaxanthin in Haematococcus pluvialis [J].Acta Hydrobiol.Sinica,2000,24(3):208-212[庄惠如,施巧勤,卢海声,等.营养胁迫对雨生红球藻虾青素累积的影响,水生生物学报,2000,24(3):208-212][10] Huang F,Lin S Z,Zhang J The growth of H.pluvialis and accumulation of astaxanthin[J]J.of Central China Normal University (Nat.Sci),1998,16(1):102-105[黄仿,林淑注,张健.雨水血球藻的生长及虾青素的积累,广西师范大学学报(自然科学版)1998,16(1):102-105][11] Zhuang H R,Lu H S,Chen B L,et al.Astaxanthin accumulation in vegetative cell of Haematococcus pluvialis[J]Acta Hydrobiol.Sinica,2001,25(4):376-380[庄惠如,卢海声,陈必链,等.雨生红球藻营养细胞的虾青素累积,水生生物学报 2001,25(4):376-380][12] Boussiba S,Vonshak A.Astaxanthin accumulation in the green alga Haematococcus pluvialis[J]Plant Cell Physiol.1991,323:1077-1082[13] Mayne S T,Parker R S.Cis-canthaxanthin and other carotenoid-like compounds in canthaxanthin preparations[J].J.Agri.Food Chem.1988,36:478[14] Jone A Bon,Timothy D.Leathers.et al.Isolation of astaxanthin-overproducing mutants of phaffia rhodozyma[J]Biotechnology letters 1997,19(2):109-112[15] Phang S M,Lee Y K.,Borouitzka M.et al.Algal Biotechnology in Asia-Pacific Region[M] Kuala Lumpru:University of Malaya.1994,130-134[16] Boussiba S,Lu fan.Enhancement and determination of astaxanthin accumulation in green alga H.pluvialis[J].Methods in Enzymology 1993,213:386-391[17] Davies B H."Chemistry and biochemistry of plant pigments" [M].London:Academic Press,1976,2:38-165[18] Mark Harker,Alex J.,Tsavalos Andrew J.Young Use of response surface methodology to optimize carotenogenesis in the microalga,Haematococcus Pluvialis [J]J.Appl.Phycol.1995,7:339-406[19] Lu F,Vonshak A,Boussiba S.Effect of temperature and irradiance on growth of Haematococcus Pluvialis(Chlorophyceae)[J].J.Phycol.,1994,30:829-833[20] Michael A,Borowitzka.John M,Huisman Ann Osborn.Culture of the astaxanthin-producing green alga Haematococcus Pluvialis I.Effect of nutrients on growth and cell type.[J].J.Appl.Phycol.1993,3:295-304[21] Yin M Y,Liu J G,Zhang J P,et al.The review about H.pluvialis and astaxanthin[J].Transactions of Oceanology and Limnology 1998,2:51-62[殷明焱,刘建国,张京浦等.雨生红球藻和虾青素研究述评.海洋湖沼通报,1998,2:51-62][22] Qiu B S,Lin Q F.An improvement on the Growth Medium for Haematococcus pluvialis [J].Acta Hydrobiol.Sinica,1999,23(4):391-394[邱保胜,刘其芳.雨生红球藻培养基的改良.水生生物学报,1999,23(4):391-394] Grung M,D'Souza F M L,Borowitzka M,et al.Algal carotenoids 51.Secondary carotenoids 2.Haematocuccus pluvialis aplanospores as a source of (3S,3′S)-astaxanthin esters[J].J Appl Phycol,1992,4:165-171[2] Lee Y K,Soh C W.Accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta)[J].J Phycol,1991,27:575-577[3] Sommer T R,Potts W T.Utilization of microalgal astaxanthin by rainbow trout (oncorhynchus mykiss)[J].Aquaculture 1991,94:79-88[4] Kranzfelder J A.Carotenoids from microalgae[J].J Phycol,1992,28(suppl.):11[5] Wang Y Q,Li Q S,The studying progress,production and application of natural carotenoids[M]Beijing;China Medical pharmacy and Science Publishing house 1997[王业勤,李勤生.天然类胡萝卜素--研究进展、生产、应用.北京:中国医药科技出版社,1997][6] Terao J.Antioxidant activity of β-carotene-related carotenoids in solution[J].Lipids 1989,24:659-661[7] Miki W.Biological functions and activities of animal carotenoids[J].Pure Appl.Chem, 1991,63:141-146[8] Shimidzu N.Carotenoids as single oxygen quenchers in marine organisms [J].Fisheries Science 1996,62:134-137[9] Zhuang H R,Shi Qr Q,Lu H S,et al.The effect of nutritional stresses on accumulation of astaxanthin in Haematococcus pluvialis [J].Acta Hydrobiol.Sinica,2000,24(3):208-212[庄惠如,施巧勤,卢海声,等.营养胁迫对雨生红球藻虾青素累积的影响,水生生物学报,2000,24(3):208-212][10] Huang F,Lin S Z,Zhang J The growth of H.pluvialis and accumulation of astaxanthin[J]J.of Central China Normal University (Nat.Sci),1998,16(1):102-105[黄仿,林淑注,张健.雨水血球藻的生长及虾青素的积累,广西师范大学学报(自然科学版)1998,16(1):102-105][11] Zhuang H R,Lu H S,Chen B L,et al.Astaxanthin accumulation in vegetative cell of Haematococcus pluvialis[J]Acta Hydrobiol.Sinica,2001,25(4):376-380[庄惠如,卢海声,陈必链,等.雨生红球藻营养细胞的虾青素累积,水生生物学报 2001,25(4):376-380][12] Boussiba S,Vonshak A.Astaxanthin accumulation in the green alga Haematococcus pluvialis[J]Plant Cell Physiol.1991,323:1077-1082[13] Mayne S T,Parker R S.Cis-canthaxanthin and other carotenoid-like compounds in canthaxanthin preparations[J].J.Agri.Food Chem.1988,36:478[14] Jone A Bon,Timothy D.Leathers.et al.Isolation of astaxanthin-overproducing mutants of phaffia rhodozyma[J]Biotechnology letters 1997,19(2):109-112[15] Phang S M,Lee Y K.,Borouitzka M.et al.Algal Biotechnology in Asia-Pacific Region[M] Kuala Lumpru:University of Malaya.1994,130-134[16] Boussiba S,Lu fan.Enhancement and determination of astaxanthin accumulation in green alga H.pluvialis[J].Methods in Enzymology 1993,213:386-391[17] Davies B H."Chemistry and biochemistry of plant pigments" [M].London:Academic Press,1976,2:38-165[18] Mark Harker,Alex J.,Tsavalos Andrew J.Young Use of response surface methodology to optimize carotenogenesis in the microalga,Haematococcus Pluvialis [J]J.Appl.Phycol.1995,7:339-406[19] Lu F,Vonshak A,Boussiba S.Effect of temperature and irradiance on growth of Haematococcus Pluvialis(Chlorophyceae)[J].J.Phycol.,1994,30:829-833[20] Michael A,Borowitzka.John M,Huisman Ann Osborn.Culture of the astaxanthin-producing green alga Haematococcus Pluvialis I.Effect of nutrients on growth and cell type.[J].J.Appl.Phycol.1993,3:295-304[21] Yin M Y,Liu J G,Zhang J P,et al.The review about H.pluvialis and astaxanthin[J].Transactions of Oceanology and Limnology 1998,2:51-62[殷明焱,刘建国,张京浦等.雨生红球藻和虾青素研究述评.海洋湖沼通报,1998,2:51-62][22] Qiu B S,Lin Q F.An improvement on the Growth Medium for Haematococcus pluvialis [J].Acta Hydrobiol.Sinica,1999,23(4):391-394[邱保胜,刘其芳.雨生红球藻培养基的改良.水生生物学报,1999,23(4):391-394]
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