免疫组化法检测美国青蛙组织中的蛙虹彩病毒
DETECTION OF RANA GRYLIO VIRUS)RGV)IN HOST FROG TISSUES BY USING IMMUNOHISTOCHEMISTRY ASSAY
-
摘要: 分不同时间,收集经人工感染了蛙病毒的样蛙,取其心、肺、肾、肠、脾、肝六种组织 ,并通过免疫组化方法进行检测.结果分别在感染了病毒3d、9d、11d后的幼蛙这六种组织中,由表及里观察到了深色的阳性信号,从而测定了病毒在入侵组织中的存在部位.其中, 肺和肠组织中阳性信号最强,呈灶性分布,其余四种组织中的阳性信号则呈散在分布.在未注射病毒的幼蛙阴性对照组六种组织中没有检测到阳性信号.Abstract: Immunohistochemistry assay has been used for detecting RGV in heart,lung,kidney,intestine,spleen,liver from the artificially infected juvenile frogs.The results showed RGV was detected in these tissues of juvenile frogs at 3d,9d,11d after infection.No positive signs were observed in these tissues from negative control.Strong positive signs were detected in lung and intestine tissue specimens,distributing in local.However the positive signs distributed diffusely in the other four tissues.
-
Keywords:
- Immunohistochemistry assay /
- Detection /
- Rana grylio virus
-
-
[1] 张奇亚,李正秋,江育林,等.沼泽绿牛蛙病毒的分离及其细胞感染的初步研究[J].水生生物学报,1996,20(4):390-392[2] Zhang Qi-Ya,Li Zhen-Qiu,Gui Jian-Fang.Studies on morphogenesis a nd cellular interactions of Rana grylio virus in an infected fish cell line [J].Aquaculture,1999,175:185-197[3] Sano N,Sano M,Sano T,et al.Herpesvirus cyprini:detection of the vi ral genome by in situ hybridization[J].Journal of Fish Diseases,1992,15:153-162[4] Sithigorngul P,Chauychuwong P,Sithigorngul W,et al,Development of a monoclonal antibody specific to yellow head virus(YHV)from Penaeus monodon [J].Disease of Aquatic Organisms,2000,42:27-34[5] Villoing S,Castric J,Jeffroy J,et al.An RT-PCR-based method for t he diagnosis of the sleeping disease virus in experimentally and naturally infec ted salmonids [J].Disease of Aquatic Organisms,2000,40:1 9-27[6] 张奇亚.特种水产动物病毒病的研究[D].中国科学院理学博士学位论文,19 97,51-75[7] 江庆萍,周慕NFDD3,林汉良,等.T细胞性淋巴瘤组织CD56的检测与EB病毒的关系[J].中华病理学杂志,1998,27(4):262-264[8] 张奇亚,李正秋.中华鳖病毒病的组织病理研究[J].水生生物学报,1997, 21(4):375-379[9] 张奇亚,李正秋.鳜鱼病毒病原的检出及组织病理分析[J].水生生物学报, 1999,23(2):151-154 张奇亚,李正秋,江育林,等.沼泽绿牛蛙病毒的分离及其细胞感染的初步研究[J].水生生物学报,1996,20(4):390-392[2] Zhang Qi-Ya,Li Zhen-Qiu,Gui Jian-Fang.Studies on morphogenesis a nd cellular interactions of Rana grylio virus in an infected fish cell line [J].Aquaculture,1999,175:185-197[3] Sano N,Sano M,Sano T,et al.Herpesvirus cyprini:detection of the vi ral genome by in situ hybridization[J].Journal of Fish Diseases,1992,15:153-162[4] Sithigorngul P,Chauychuwong P,Sithigorngul W,et al,Development of a monoclonal antibody specific to yellow head virus(YHV)from Penaeus monodon [J].Disease of Aquatic Organisms,2000,42:27-34[5] Villoing S,Castric J,Jeffroy J,et al.An RT-PCR-based method for t he diagnosis of the sleeping disease virus in experimentally and naturally infec ted salmonids [J].Disease of Aquatic Organisms,2000,40:1 9-27[6] 张奇亚.特种水产动物病毒病的研究[D].中国科学院理学博士学位论文,19 97,51-75[7] 江庆萍,周慕NFDD3,林汉良,等.T细胞性淋巴瘤组织CD56的检测与EB病毒的关系[J].中华病理学杂志,1998,27(4):262-264[8] 张奇亚,李正秋.中华鳖病毒病的组织病理研究[J].水生生物学报,1997, 21(4):375-379[9] 张奇亚,李正秋.鳜鱼病毒病原的检出及组织病理分析[J].水生生物学报, 1999,23(2):151-154
计量
- 文章访问数: 1033
- HTML全文浏览量: 0
- PDF下载量: 470
下载: