基于PCR-DGGE指纹图谱川纹笛鲷及圆白鲳消化道壁优势菌群结构比较分析
COMPARISON OF THE PREDOMINANT BACTERIAL COMMUNITY STRUCTURE IN THE GASTROINTESTINAL WALL BETWEEN LUTJANUS SEBAE AND EPHIPPUS ORBIS BASED ON16S rDNA PCR 2DGGE FINGERPRINT
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摘要: 本文采用免培养的16SrDNA梯度凝胶电泳技术(DGGE)对集约化海水网箱养殖川纹笛鲷Lutjanussebae及圆白鲳Ephippusorbis消化道壁优势菌群结构进行了比较分析。研究结果显示川纹笛鲷及圆白鲳消化道壁存在着大量细菌群落,对DGGE指纹图谱聚类分析表明两种鱼肠道壁及胃壁菌群组成相似度高于50%,其中二者肠道壁细菌组成相似性最高(67%),这些可能与两种鱼养殖在同一水域、摄食相同饵料相关,另外通过软件对DGGE指纹带谱相对丰度分析表明同种鱼肠道壁及胃壁具有相同最大优势菌群。同时,两种鱼消化道壁之间在细菌多样性及相对丰度上亦存在明显区别,圆白鲳消化道壁细菌多样性要高于川纹笛鲷,这可能归因于川纹笛鲷与圆白鲳在天然环境中栖息地的差异性。本研究通过首次建立不同海水鱼消化道壁16SrDNA-DGGE指纹图谱及比较分析,为澄清海水鱼消化道壁微生物区系奠定基础。Abstract: The comparison of the predominant bacterial community structure in the gastrointestinal walls between Lutjanus sebae (Cuvier, 1816) and Ephippus orbis (Bloch, 1787) based on 16S rDNA PCR2DGGE fingerprint using a culture independent method was conducted in the paper. The results showed that abundant bacteriumwere planted in the gastrointestinal walls of both marine finfish species, the similarity was above 50% in the community structures in the gastrointestinal walls with the highest similarity of 67% in the bacterial composition between the intestinal wall of Lutjanus sebae and that of Ephippus orbis, and the walls of the stomach and the intestinal in one fish shared the same most predominant bacteria species, which might reflect the same aquaculture environment and the diet fedfor bothfinfish species. However, the difference of the richness and relative abun dance in the bacterium of the gastrointestinal walls in both finfish species was also observed from the DGGEfingerprint with the results of richer bacterium of the gastrointestinal wall in Ephippus orbis compared to that in Lutjanus sebae, which might due to the different natural living habits. The first time of the establishment and comparison of the 16S rDNA2DGGEfingerprint in the gastrointestinal wall of marine finfish in present study do help to elucidate the microflora structure in the gastrointestinal wall of marine finfish.
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Keywords:
- Lutjanus sebae /
- Ephippus orbis /
- 16S rDNA /
- DGGEfingerprint
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