Cu2+、Zn2+诱导稀有鮈鲫应激蛋白质的研究
THE INDUCTION OF STRESS PROTEIN IN GROBIOCYPRIS RARUS BY Cu2+ AND Zn2+
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摘要: 以稀有鮈鲫为材料,研究了应激蛋白质作为生物学指标的敏感性.结果表明,在无可观察效应浓度下,经5d亚慢性胁迫暴露,以Cu2+为胁迫因子,稀有鲫被诱导出约54KDa的应激蛋白质;以Zn2+为胁迫因子,稀有鲫被诱导出约94KDa,67KDa和40KDa的应激蛋白质.应激蛋白质有可能成为一种生物学指标运用于生态风险性早期预警.Abstract: The sensitivity of stress protein as a biomarker was researched in the project. The experiment was initiated using 3d old Grobiocypris rarus larvae according to subchronic toxicity test and run for 5d. Special stress proteins of approximately 54KDa or 94KDa, 67KDa and 40KDa were induced in response to Cu2+ or Zn2+ at the No Observable Effect Concentration, respectively. Compared with the conventional end points such as growth, this assay is a more sensitive and rapid indicator. Therefore, stress protein might be an useful biomarker as an early warning system in risk assessment.
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Keywords:
- Grobiocypris rarus /
- Stress protein /
- Biomarker /
- Sensitivity
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