细胞松弛素B对日本沼虾四倍体的适宜诱导条件的研究
STUDY ON INDUCEMENT CONDITION OF TETRAPLOID IN MACROBRACHIUM NIPPONENSE BY CYTOCHALASIN B
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摘要: 本文研究了用细胞松弛素B(Cytochalasin B,CB)诱导日本沼虾(Macrobrachium nipponense)多倍体的处理条件和方法。试验水温分别为18-19℃、22-23℃,CB浓度为1.0-2.0mg/L,处理的起始时间在受精后5-9h,处理的持续时间为10-20min。结果表明,在水温为18-19℃时,四倍体卵子的最适诱导条件为:受精后7-8h,CB溶液的浓度1.5mg/L,处理20min,四倍体诱导率最高可达56.1%;在水温为22-23℃时,最适诱导条件为:在受精后6 h左右,CB溶液浓度为1.5mg/L,处理15-20min,四倍体诱导率最高可达54.3%。处理的起始时间和CB溶液的浓度对胚胎的四倍体率有显著影响(P0.05);胚胎的成活率随CB浓度的提高与处理的持续时间的延长而下降。考虑到胚胎成活率因素,建议选择CB浓度为1.5mg/L,处理持续时间15-20min。Abstract: Cytochalasin B (CB) was used for inhibiting the first cleavage of zygotes to induce tetraploid in Macrobrachium nippo-nense. At 18-19℃ and 22-23℃ water temperature, two tetraploid induction trials designed by L9(34) were carried out using CB treatment at different beginning time after fertilization (5 hours, 6 hours, 7 hours, 8 hours, 9 hours), different concentration of CB (110mg/L, 115mg/L,210mg/L), and different treatment duration time of the eggs (1.0 min, 1.5 min, 2.0 min)1 The re-sults indicated that the highest induction rate at 18-19℃and 22-23℃ were 56.1% and 54.3% respectively. The optimum condition of inducement tetraploid at 18-19℃ was that treatment beginning timewas 7-8 hours after fertilization, CB concen-tration was 1.5mg/L, and treatment duration was 20min. The optimum condition of inducement tetraploid at 22-23 e was that treatment beginning timewas 6 hours after fertilization, CB concentration was 115mg/ L, and treatment duration was 15-20min. The lowest induction rate at 18-19℃and 22-23℃were 28.5% and 20.1% respectively at the experimental condition of 1.0mg/ L CB concentration, beginning treatment at 5-7 hours after fertilization, and 10 min treatment duration time1 Beginning time of CBtreated and the concentration of CB significantly affected the induction rate of tetraploid (P 0.05). Significantly different hatching rate were observed between three different CB concentration (P< 0.05). As the increase of CB concentration, the damage of embryo was increased, the hatching rate was reduced (P< 0.05). Combining to induction rate of tetraploid, the survival rate of embryo and hatching rate, the treatment condition of 1.5mg/L of CB concentration and 20min treatment duration were recommended1
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Keywords:
- Macrobrachium nipponense /
- Embryo /
- Tetraploid /
- Cytochalasin B(CB)
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[1] [1] Wu Y P, Ye Y Z, Wu Q J. The expression patterns of genes in dif-ferent biotypes by genome addition[J]. Acta Genetica Sinica, 2000,27(2) : 108) 113 [吴玉萍, 叶玉珍, 吴清江. 基因叠加生物型的基因表达特征. 遗传学报,2000, 27(2) :108) 113]
[2] [11] Lu J P. Nucleus behav iour in fertil ized eggs of the fresh-water prawnMacrobrachium nipponense [J]. Dongha Mauine Science,2001,19(4) :17) 20[卢建平. 日本沼虾受精卵核行为的初步研究.东海海洋, 2001, 19(4) : 17) 20]
[2] Schmidtke J. Gene action in fish of tetraploid origin. ò Cellular andbiochemical parameters in clupeoid and salmonid fish [J]. BiochemGenet, 1976, 13: 301
[3] Danzmann R G, Bogart J P. Gene dosage effects on MDH isozymeexpression in diploid, triploid, and tetraploid treefrogs of the genus Hyla[J]. J Heredity, 1982, 73: 277) 280
[4] Beck M L. Chromosomal and electrophoretic analyses of hybrids be-ween grass carp and bighead carp[J]. Copeia, 1984, 81: 337) 342
[5] Bao Z M, Zhang Q Q, Wang H, et al. Studies on the triploidy in-duction in Fenneropenaeus Chinensis, ò. Treated by cytochalasin B[J]. Marine Science, 1990, 15(3) : 101) 105[包振民, 张全启,王海等. 中国对虾三倍体的诱导研究ò : 细胞松弛素 B处理.海洋学报, 1990, 15(3) : 101) 105]
[6] Dai J X, Bao Z M, Zhang Q Q, et al. Studies on the triploidy in-duction in Fenneropenaeus Chinensis,?. Treated by heat shock J]. Hereditas, 1993, 15(5) : 15) 18[戴继勋, 包振民, 张全启.中国对虾三倍体的诱发研究? : 温度休克. 遗传, 1993, 15(5) :5) 18]
[7] Lu R H. An experiment on induction of triploidy in Eriocheir japoni-cus hepuensis by heat treatment.Chin J Oceanol Limnol, 1993.11(1) :21) 24
[8] Chen L Q, Zhao Y L, Wang Y F, et al. Studies onthe polyploid in-duction in the chinese mitten-handed crab, eriocheir sin ensis,?.Induction of triploidy and tetraploidy embryos in eriocheir sinensis bycytochalasin B[J]. J Fisheries China, 1997, 21(1) : 19) 25
[10] Qiu G F, Du N S, Lai W. Chromosomal and karyological studies onthe freshwater prawn Macrobrachium Nipponenses (Crustacea, De-capoda) [J]. Oceanologia et Limnologia Sinica, 1994, 25(5) : 493)498[邱高峰, 堵南山, 赖伟. 日本沼虾染色体及其核型的研究. 海洋与湖沼, 1994, 25(5) : 493) 498]
[12] Zhao Y L, Meng F L, Chen L Q, et al. Effects of different gradienttemperatures on embryonic development of the Cherax Quadricarinatus(Crustacea, Decapoda) [J]. J Lake Sciences, 2000, 12(1) : 59) 62[赵云龙, 孟凡丽, 陈立侨等. 不同水温对红螯螯虾胚胎发育的影响, 湖泊科学,2000, 12(1) : 59) 62]
[13] Li B, Wang Z P, Wang R C. Variance of triploid rate of induced pa-cific oyster crassostrea gigas during development[J]. J OceanUniver-sity of Qingdao, 2001, 31(5) : 666) 672[李斌, 王昭萍, 王如才.诱导三倍体太平洋牡蛎群体发育过程中三倍体率的变化. 青岛海洋大学学报,2001, 31(5) : 666) 672]
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