黄喉拟水龟(♀)与三线闭壳龟(♂)杂交后代的形态特征及其与父母本的比较研究
THEMORPHOLOGIC CHARACTERS OF HYBRID (MAUREM YS MUTICA ♀ ×CUORA TRIFASCIATA ♂) AND COM PARISONW ITH THEIR PARENTS
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摘要: 本研究通过黄喉拟水龟Mauremys mutica(♀)与三线闭壳龟Cuora trifasciata(♂)进行杂交，成功获得了杂种龟。这说明黄喉拟水龟和三线闭壳龟是可以进行属间远缘杂交的，但杂交组合的受精率及孵化成功率均低于黄喉拟水龟的同种组合。杂种稚龟与黄喉拟水龟稚龟在背甲纹路、腹甲黑斑、四肢和尾腹面的皮肤颜色、喉盾前端形状及起点位置、喉盾宽／喉盾缝长、喉盾缝长／肱盾缝长、股盾缝长／肛盾缝长存在差异。1龄前，杂种龟生长快于黄喉拟水龟。形态特征上，杂种龟头顶部淡棕黄色，头侧眼后有两条黑色纵纹，颈腹部淡黄色;背甲棕色，腹甲浅黄色，每一盾片中间都有边缘呈放射状的黑斑;四肢、尾腹面及裸露皮肤部分为黄褐色。形态可量数据分析显示杂种龟在形态上接近黄喉拟水龟。建立了三种龟的形态判别公式，判别的准确率为100%(p＜0.01)，判别分析中贡献最大的4个变量分别是腹甲后半部长／背甲长、喉盾宽／背甲长、肱盾缝长／背甲长、股盾缝长／背甲长，可见三线闭壳龟、黄喉拟水龟与杂交种腹部的腹甲后半部长、喉盾宽、肱盾缝长、股盾缝长等参数可作为鉴别三者的直接依据。本实验的结果对杂种龟的鉴定、龟类的杂交育种及养殖生产实践都有一定的借鉴作用。Abstract: Yellow pond turtle, M auremys mutica and three-striped box turtle, Cuora trifasciata, is classified to Bataguridae, M auremys and Cuora, respectively, they distribute in Southern China1 The two turtles have been artificially-propagated successfully and were cultivated in a small-scale. M auremys iversoni is a new strain nominated by Pritchard in 1991,but in 2001, two research groups leading by Parham and Wink proved that M auremys iversoni was not a species1With different methods, they found that M auremys iversoni may be the hybrid of yellow pond turtle and three2striped box turtle1 In this article, the hybrid of M. mutica (♀) ×C. trifasciata (♂) was acquired successfully, which indicated the hybrid between genus of M auremys and Cuora was feasible. But the rate of fecundation and hatching of M. mutica (♀) ×C.trifasciata (♂) were lower than M. mutica (♀) ×M. mutica (♂). The veins on carapace, the dark patch on plastron, the forepart form and origination of gular scute, the ratio between gular scute width and gular scute seam length, gular scute seam length and humeral scute seam length, femoral scute seam length and anal scute seam length, the skin color of inside of four limbs and tail abdomen of the young hybrid were different from the youngM. mutica. The growth rate of the one year old hybrid was faster than M. mutica in the same culture condition. In morphology of hybrid, the color of calvarias was thin brown-yellow, and it has two black lines behind the eyes, the abdomen of neck was yellow; the carapace was brown and its plastron was thin yellow, each scute of plastron has radiated dark patch, the skin color of inside of four limbs and tail abdomen of the hybrid were yellow-brown. The analysis ofmorphologic data within three kinds of turtles indicated that the hybrid was more closed to itsmother, M. mutica. Morphologic discriminant formula of three turtleswere obtained, discriminant analysis indicated that there were significant different among three kinds turtles1 The identification accuracy was 100% (p < 0101). The ratio between second half plastron length, gular scute width, humeral scute seam length, femoral scute seam length, respectively, and carapace length were the highest contributing rate on discriminant analysis, which could be the direct parameter in distinguishing yellow pond turtle, three-striped box turtle and their hybrid. The studywould be benefit to identification of hybrid, turtle breeding and aquaculture.
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Keywords:
- M auremys mutica /
- Cuora trifasciata /
- Hybrid /
- Morphologic characters
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[1] 科学出版社. 1998]
[1] Zhao E M1 The red book about severe danger animals in China: Amphibians and reptiles [M].Beijing: Science Press1 1998 [赵尔宓.中国濒危动物红皮书: 两栖类和爬行类.北京:
[2] Zhu X P, Chen YL, Liu Y H, et al. Comparative study of the growth of M auremysmutica, Cuora trifasciata and Chlyre serpentine [J]. Journal of Fisheries of China, 2001, 25(6) : 507-511[朱新平, 陈永乐, 刘毅辉, 等.黄喉拟水龟、三线闭壳龟、鳄龟的生长比较.水产学报, 2001, 25 (6) : 507-511]
[2] EastAsia [M].Kyoto: Proceedings of the second Japan-China herpetological symposium1 1989, 193-197
[3] Zhu X P, Chen Y L, Wei C Q, et al. Reproduction of yellow pond turtle, M auremys mutica [J]. Acta Hydrobiologica Sinica, 2001, 25 (5) : 454-460 [朱新平, 陈永乐, 魏成清, 等.黄喉拟水龟的繁殖生物学研究.水生生物学报, 2001, 25 (5) : 454-460]
[4] Zhu X P, Chen YL, Wei C Q, et al. Effects of artificial rearing on fecundity ofM auremys mutica Cantor [J]. Journal of Fishery Sciences of China, 2001, 8 (2) : 52-54 [朱新平, 陈永乐, 魏成清, 等.人工饲养对黄喉拟水龟繁殖力的影响.中国水产科学, 2001, 8 (2) : 52-54]
[5] HongM L, WangL J, ShiH T. The biological character and domestic breeding of the three-lined box turtle [J]. Journal of Hainan Normal University (Natural Science), 2003, 16 (3) : 78-82 [洪美玲, 王力军, 史海涛.三线闭壳龟的生物学特性及人工养殖. 海南师范学院学报 (自然科学版), 2003, 16 (3) : 78-82]
[6] Kou Z1 Cyclemys from Yunnan, a description of a new species and a new record to China (Testudinata: Emydidae) [A]. In: MatsuiM, Hikida T, Goris R C (Eds1), Current herpetology in
[7] Parham J F, SimisonW B, Kozak K H, et al. New Chinese turtles: endangered or invalid? A reassessment of two species using mitochondrialDNA, allozyme electrophoresis and known-locality specimensAnimal [J]. Conservation, 2001, 4: 357-367
[8] Dalton1 Rex.Mock turtles [J]. Nature, 2003, 423: 219-220
[9] Pritchard P C H, McCordW P. A new emydid turtle from China [J]. Herpetologica, 1991, 47: 139-147
[10] W inkM, GuickingD and FritzU. Molecular evidence for hybrid origin of M auremys iversoni Pritchard et McCord, 1991, and M auremys pritchardi McCord, 1997 (Retilia: Testudines: Bataguridae) [J]. Zoologische Abhandlungen, Staatliches M useum für Tierkunde Dresden, 2001, 51: 41-49
[11] Spinks P Q, Shaffer H B, Iverson J B, et al. Phylogenetics hypotheses for the turtle family Geoemydidae [J].Molecular Phylogenetics and Evolution, 2004, 32: 164─182
[12] Parham J F and Shi H1 The discovery of M auremys iversoni-like turtles at a turtle farm in Hainan Province, China: The counterfeit golden coin [J]. Asiatic Herpetological Research, 2001, 9: 71-77
[13] Iverson J B andMcCord W P. Variation in East Asian turtles of the genus M auremys (Bataguridae: Testudines) [J]. Journal of Herpetology, 1994, 28: 178-187
[14] Zhang E, Xie Z G, Xie C X. Morphological variation between Sinabrama macrops and s1 wui, with notes on their validities [J]. Acta Hydrobiologica Sinica, 2000, 28 (5) : 511-518 [张鹗, 谢仲桂, 谢从新.大眼华鳊和伍氏华鳊的形态差异及其物种有效性.水生生物学报, 2000, 28 (5) : 511-518]
[15] Zhou T. Atlas for Identification of Turtles and Tortoises [M].Beijing: China Agriculture Publishing House1 2004, 115 [周婷. 龟鳖分类图鉴.北京: 中国农业出版社. 2004, 115]
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