植酸酶对斑点叉尾生长性能及磷当量的研究
STUDY ON GROWTH PERFORMANCE AND PHOSPHORUS EQUIVALENT OF PHYTASE IN CHANNEL CATFISH (ICTALURUS PUNCTATUS)
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摘要: 试验旨在研究饲料中添加植酸酶对斑点叉尾[初始平均体重(1.70±0.04) g]生长性能及植酸磷利用的影响, 确定植酸酶的磷当量。试验采用单因素试验设计, 以Ca(H2PO4)2 提供外源无机磷, 同时添加不同浓度植酸酶, 试验设计为10 个处理组, 分别为1 个对照试验组、4 个无机磷试验组(0.3%、0.5%、0.8%、1.2%)和5 个植酸酶试验组(300、500、1000、1500、2000 U/kg), 每个处理3 个重复, 每个重复30 尾鱼。通过折线模型确定植酸酶的最佳添加量; 通过回归分析, 建立响应指标(特定生长率、椎骨磷)与外源磷添加量之间的线性关系, 进而确定植酸酶的磷当量值。结果表明: (1)添加植酸酶的处理组与对照组相比, 增重率、特定生长率、蛋白质效率、肥满度均有显著提高(PPP>0.05); 鱼体粗蛋白、粗灰分、钙、磷及椎骨粗灰分、钙、磷均有显著提高(PY1=0.2714X+2.294(X-无机磷, Y1-特定生长率, R2=0.9238), 300、500、1000、1500 和2000 U/kg 植酸酶分别可替代1 kg 饲料中0.13%、0.57%、0.76%、1.46% 和1.35%的磷酸二氢钙, 等效于添加了0.03%、0.14% 、0.19%、0.36%和0.33%的有效磷;Y2=0.8737X+5.1028(X-无机磷, Y2-椎骨磷, R2=0.9638), 300、500、1000、1500 和2000 U/kg 植酸酶分别可替代1 kg 饲料中0.47%、1.11%、1.18%、1.38%和1.41%的磷酸二氢钙, 等效于添加了0.12%、0.27%、0.29%、0.34%和0.35%的有效磷。在试验条件下, 添加1000―2000 U/kg 植酸酶能有效改善斑点叉尾生长性能, 有利于营养物质在鱼体中的沉积, 促进骨骼矿化。以特定生长率为响应指标, 植酸酶最佳添加量为1435 U/kg等效于添加了0.37%的有效磷; 以椎骨磷为响应指标, 植酸酶最佳添加量为1226 U/kg 等效于添加了0.33%的有效磷。Abstract: The trial was conducted to study the effects of phytase on growth performance and determine the phosphorus equivalent of phytase in channel catfish (initial average weight about 1.70 g). Single factor design was used for the trial, Ca(H2PO4)2 was provided in four inorganic phosphorus diets (0.3%, 0.5%, 0.8% and 1.2%), and different levels of phytase were sprayed on basal diet (300, 500, 1000, 1500 and 2000 U/kg). To determine the optimum additive amount of phytase by line model, fish of homogeneous sizes were randomly allotted to 10 dietary treatments with triplicates of 30 fish in each tank. The linear relationships were established between supplemented P and response parameters (specific growth rate and vertebra bone phosphorus) to evaluate phytase phosphorus equivalent. The results showed that: (1) Compared with the basal group, growth rate, specific growth rate, protein efficiency ratio and condition fatness were increased significantly (PPPY1=135.94X+7.8316 (X-inorganic p, Y1-SGR, R2=0.9453), the phosphorus equivalency [Ca(H2PO4)2] of 300, 500, 1000, 1500 and 2000 U/kg diet phytase was 0.13%, 0.57%, 0.76%, 1.46% and 1.35% respectively, and equal to 0.03%, 0.14%, 0.19%, 0.36% and 0.33% available phosphorus added to basal diet; Y2=0.8737X+5.1028 (X-inorganic p, Y2- vertebra phosphorus, R2=0.9638), the phosphorus equivalency [Ca(H2PO4)2] of 300, 500, 1000, 1500 and 2000 U/kg diet phytase was 0.47%, 1.11%, 1.18%, 1.38% and 1.41% respectively, and equal to 0.12%, 0.27%, 0.29%, 0.34% and 0.35% available phosphorus added to basal diet. In conclusion, 1000 U/kg-2000 U/kg phytase supplemented in diet significantly improved growth performance, conduced to the deposition of nutriment in body and bone mineralization. The phosphorus equivalency of 1435 U/kg diet phytase was the optimum and equal to 0.37% available phosphorus added to basal diet if mean specific growth rate was as reference index, then the phosphorus equivalency of 1226 U/kg diet phytase was the optimum and equal to 0.33% available phosphorus added to basal diet if mean specific growth rate was as reference index.
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[1] [余丰年, 王道尊. 植酸酶对异育银鲫生长及饲料中磷利用率的影响. 中国水产科学, 2000, 7(2): 106-109]
[1] Huo Q G. Phosphorus Nutrition and Source of Animal ［M］.Beijing: Agricultural Science and Technology of China Press.2002, 1 [霍启光. 动物磷营养与磷源. 北京: 中国农业科学技术出版社. 2002, 1]
[2] Guo L Y. Study on the application of replacement monocalciumphosphate with micro-acidic phytase in the diets ofthe gibel carp (Carassius aruatus gibelio) ［D］. Thesis forMaster of Science. Chinese Academy of Agricultural Sciences,Beijing. 2009 [郭利亚．酸性植酸酶在异育银鲫饲料中替代磷酸二氢钙的应用研究. 硕士学位论文, 中国农业科学院, 北京. 2009]
[3] He J H. Recent advances in phytate and phytase studies ［J］.Acta Zoonutrimenta Sinica, 2005, 17(1): 1-6 [贺建华. 植酸磷和植酸酶研究进展. 动物营养学报, 2005, 17(1):1-6]
[4] Maenz D D, Classen H L. Phytase activity in the small intestinalbrush border membrane of the chicken ［J］. Poultry Science,1998, 77(4): 557-563
[5] Zhang L, Ai Q H, Mai K S, et al. Effects of phytase andnon-starch polysaccharide enzyme supplementation in dietson growth and digestive enzyme activity for Japanese seabass(Lateolabrax japonicas) ［J］. Acta HydrobiologicaSinica, 2009, 33(1): 82-88 [张璐, 艾庆辉, 麦康森, 等.植酸酶和非淀粉多糖酶对鲈鱼生长和消化酶活性的影响.水生生物学报, 2009, 33(1): 82-88]
[6] Chen J H, Mai K S. Effects of different supplemental methodsof phytase to soybean mail on growth and feed utilizationof Japanese flounder (Paralichthys olivaceus) ［J］. ActaHydrobiologica Sinica, 2010, 34(3): 481-487 [陈京华, 麦康森. 不同添加方式植酸酶处理豆粕对牙鲆生长和饲料利用率的影响. 水生生物学报, 2010, 34(3): 481-487]
[7] Yu F N, Wang D Z. Effects of microbial phytase on growthand utilization of phosphorus in Carssiu auratus gibelio ［J］.Journal of Fishery Sciences of China, 2000, 7(2): 106-109
[8] Luo L, Wu X F, Xue M, et al. Effect of replacement calciumphosphate monobasic with neutral phytase on growth andphosphorous metabolism of Japanese sea bass (Lateolabraxjaponicus) ［J］. Chinese Journal of Animal Nutrition, 2007,19(1): 33-39 [罗琳, 吴秀峰, 薛敏, 等. 中性植酸酶在豆粕型饲料中替代磷酸二氢钙对花鲈生长及磷代谢的影响.动物营养学报, 2007, 19(1): 33-39]
[9] Zheng T, Pan Q, Li G F, et al. Effects of phosphorus andphytase supplements on growth performance and body compositionin juvenile tilapia Oreochromis niloticus×O. aureusfed fishmeal-free diet ［J］. Journal of Fishery Sciences ofChina, 2006, 13(1): 112-118 [郑涛, 潘庆, 李桂峰, 等.无鱼粉饲料中添加磷和植酸酶对奥尼罗非鱼生长性能及体成分的影响.中国水产科学, 2006, 13(1): 112-118]
[10] Cao L. Effects of pretreatment with microbial phytase on growth performance and phosphorous utilization of nile tilapia(Oreochromis niloticus) ［D］. Thesis for Master of Science.Huazhong Agricultural University, Wuhan. 2007 [曹玲.植酸酶预处理对尼罗罗非鱼生长及磷代谢的影响. 硕士学位论文, 华中农业大学, 武汉. 2007]
[11] Yang Y H, Guo Q, Zu L C, et al. Effects of dietary phytaseon growth performance and phosphorus availability for juvenilecarp ［J］. Freshwater Fisheries, 2006, 36(5): 20-23 [杨雨虹, 郭庆, 祖立闯, 等. 植酸酶对鲤鱼生长及磷利用率的影响. 淡水渔业, 2006, 36(5): 20-23]
[12] NRC (National Research Council). Nutrient requirements offish, National Academy of Sciences. Committee on AnimalNutrition, Washington D.C. 1993
[13] Jackson L S, Li M H, Robinson E H. Use of microbial phytasein channel catfish (Ictalurus punctatus) diets to improveutilization of phytate phosphorus ［J］. Journal of the WorldAquaculture Society, 1996, 27: 309-313
[14] AOAC. Official Methods of Analysis of AOAC International,16th Edition ［M］. AOAC International, Arlington, VA. 1995,1094
[15] Wang Y Z, Cui S W, Wu X Q, et al. Study on measuringmethod of phytic acid in feed and grain ［J］. China Feed,1991, 6: 28-31 [王永真, 崔淑文, 吴秀琴, 等. 饲料和谷物中植酸磷测定方法研究. 中国饲料, 1991, 6: 28-31]
[16] Mroz Z, Jongbloed A W, Kemme P A. Apparent digestibilityand retention of nutrients bound to phytate complexes as influencedby microbial phytase and feeding regimen in pigs［J］. Journal of Animal Science, 1994, 72(1): 126-132
[17] Gentile J M, Roneker K R, Crowe S E, et al. Effectiveness ofan experimental consensus phytase in improving dietaryphytate-phosphorus utilization by weanling pigs ［J］. Journalof Animal Science, 2003, 81: 2751-2757
[18] Papatryphon E, Soares J H. The effect of phytase on apparentdigestibility of four practical plant feedstuffs fed to stripedbass, Morone saxatilis ［J］. Aquaculture Nutrition, 2001, 7:161-167
[19] Gao C S, Wang Y L. Effects of phytase on growth and utilizationof phosphorus in grass carp ［J］. Journal of Anhui AgriculturalSciences, 2006, 34(7): 1377-1379 [高春生, 王艳玲. 植酸酶对草鱼生长性能及植酸磷代谢的影响. 安徽农业科学, 2006, 34(7): 1377-1379]
[20] Kim J D, Lall S P. Effects of dietary protein level on growthand utilization of protein and energy by juvenile haddock(Melanogrammus aeglefinus) ［J］. Aquaculture, 2001, 195:311-319
[21] Jover M, Garcia G A, Tomas A, et al. Growth of mediterraneanyellowtail (Seriola dumerilii) fed extruded diets containingdifferent levels of protein and lipid ［J］. Aquaculture,1999, 179: 25-33
[22] Chen L, Wang T. Effects of feeding leguminous grass powderon rabbit meat quality ［J］. Acta Ecologiae Animalis Domastici,2009, 30(5): 89-93 [陈琳, 王恬. 植酸酶对肉鸭屠宰性能及血清学指标的影响. 家畜生态学报, 2009,30(5): 89-93]
[23] Niu J F, Wu R Q, Xie J, et al. Effects of phytase on growthand body content in largemouth bass Micropterus salmoides［J］. Feed Industry, 2008, 29(6): 21-23 [牛纪锋, 吴锐全,谢 骏, 等. 饲料中添加植酸酶对大口黑鲈生长及鱼体营养成分的影响. 饲料工业, 2008, 29(6): 21-23]
[24] Wei B, Robert. Effect of fungal phytase on utilization ofdietary protein and minerals, and dephosphorylation ofphytic acid in the alimentary tract of channel catfish Ictaluruspunctatus fed an all-plant-protein diet ［J］. Journal of theWorld Aquaculture Society, 2002, 33(1): 10-22
[25] Jouni Vielma. Influence of dietary soy and phytase levels onperformance and body composition of large rainbow trout(Oncorhynchus mykiss) and algal availability of phosphorusload ［J］. Aquaculture, 2000, 183: 349-362
[26] Song J C, Shan A S. Effects of phytase on utilization of proteinand amino acid ［J］. China Feed, 2000, 10: 14-15 [宋金彩, 单安山. 植酸酶对蛋白质和氨基酸利用率的影响. 中国饲料, 2000, 10: 14-15]
[27] Yi Z, Kornegay E T, Lindemann M D, et al. Effectiveness ofnatuphos phytase in improving the bioavailabilities of phosphorusand other nutrients in soybean meal-based semipurifieddiets for young pigs ［J］. Journal of Animal Science,1996, 74(7): 1601-1611
[28] Cromwell G L, Stahly T S, Coffey R D, et al. Efficacy ofphytase in improving the bioavailability of phosphorus insoybean meal and corn-soybean meal diets for pigs ［J］.Journal of Animal Science, 1993, 71(7): 1831-1840
[29] Knowlton K F, Radcliffe J S, Novak C L, et al. Animalmanagement to reduce phosphorus losses to the environment［J］. Journal of Animal Science, 2004, 82(E. Suppl.): E173-E195
[30] Biehl R R, Baker D H, Deluca H F. 1α-hydroxylat- edcholecalciferolcompounds act additively with microbial phytaseto improve phosphorus, zinc andmanganese utilization inchicks fed soy-based diets ［J］. Journal of Nutrition, 1995,125(9): 2407-2416
[31] Lei X G, Ku P K, Miller E R, et al. Supplemental microbialphytase improves bioavailability of dietary zinc to weanlingpigs ［J］. Journal of Nutrition, 1993a, 123: 1117-1123
[32] Soares J H. Efficacy of phytase on phosphorus utilization ［J］.Proceeding of Marylanp Nutrition Conference, 1994, 53:76-79
[33] Schafer A, Koppe W M. Effects of a microbial phytase onthe utilization of native phosphorus by carp in a diet basedon soybean meal ［J］. Water Science and Technology, 1995,31(10): 149-155
[34] Hua K, Bureau D P. Quantification of differences in digestibilityof phosphorus among cyprinids, cichlids, and salmonidsthrough a mathematical modelling approach ［J］.Aquaculture, 2010, 308: 152-158
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