三聚氰胺对花鲈的急性毒性实验研究

刘海燕; 张伟; 薛敏; 吴秀峰; 郑银桦; 郭利亚; 盛洪建

三聚氰胺对花鲈的急性毒性实验研究

刘海燕^1,2,
张伟^1,3,
薛敏^1, ,,
吴秀峰¹,
郑银桦¹,
郭利亚¹,
盛洪建¹

中国农业科学院饲料研究所, 国家水产饲料安全评价基地, 北京 100081
2. 河北师范大学生命科学学院, 石家庄 050016
3. 大连水产学院, 大连 116023

基金项目:

农业部专项项目

十一五国家科技支撑计划(2006BAD12B06)(2006BAD12B08)资助

计量
- 文章访问数: 995
- HTML全文浏览量: 1
- PDF下载量: 796
出版历程
- 收稿日期: 2008-09-07
- 修回日期: 2009-01-14
- 发布日期: 2009-03-24

ACUTE TOXIC ITY STUDY FORMELAM INE ON JAPANESE SEA BASS(LATEOLABRAX JAPON ICUS)

Feed Research Institute, the Chinese Academy of Agricultural Sciences, Beijing 100081;
2. The College of Life Science, Hebei Normal University, Shijiazhuang 050016;
3. Dalian Fisheries University, Dalian 116023

摘要

摘要: 本文以花鲈(Lateolabrax japonicus)为实验对象,采用接触、腹腔注射及口服三种致毒方式,进行了非蛋白氮物质-三聚氰胺对花鲈的急性毒性实验研究。口服急性毒性实验中,通过在饲料中添加不同浓度的三聚氰胺(0、500、2000、5000及10000mg/kg),进行了21d的花鲈养殖实验。实验结果表明:三聚氰胺溶解度较低,其水溶液没有表现出急性毒性,LC503500mg/L;而在腹腔注射致毒方式下,三聚氰胺对花鲈的半致死剂量LD50=890.07mg/kg·w;LD5095%可信限为:778.63-1017.45mg/kg·w;在口服急性毒性实验中,10000mg/kg组三聚氰胺降低了花鲈的摄食与生长(p0.05),饲料系数显著升高(p0.05);三聚氰胺对花鲈的存活率、肥满度、肝体比及脏体比均无显著影响(p0.05)。饲料中添加三聚氰胺没有显著影响花鲈血清的丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总蛋白、葡萄糖、胆固醇、甘油三酯及尿素氮(p0.05),但显著影响了花鲈血清的碱性磷酸酶活性,10000mg/kg组的碱性磷酸酶活性显著高于其他各处理组(p0.05),其他各组间无显著差异(p0.05)。三聚氰胺对花鲈21d的最大未观察到有害作用剂量(NOAEL,no-observed-adverse-effect-level)为131.99mg/kg·w·d。
- 三聚氰胺 /
- 花鲈 /
- 急性毒性实验
Abstract: Melamine had become the focus of feed safety assessment because of the tainted pet food and milk powder scandal happened in 2007 and 20081Melamine is a high nitrogen content industrial chemicalmaterial, but it was falsely added to protein sources to enhance the apparent p rotein content for the bigger profit. In this study, the acute toxic experiments(including touching test, injection test and oral test) were conducted on Japanese sea bass(Lateolabrax japonicus) to assess the safety of melamine in fish. In touching test, Japanese sea bass(50g±011g) were exposed to 0 and 3500mg/L melamine solution at 27℃ for 96h, no feed was provided to the experimental fish during trial, the mortality of the Japanese sea bass were calculated after 96h; In injection test, the melamine was injected into Japanese sea bass(75g±111g) at 0, 200, 360, 648, 116614 and 2099152 mg/kg·w levels from the abdomen, respectively, lasted for 14d in a recirculation rearing system, and the fish were fed once daily, the mortality of Japanese sea bass were calculated after 14d, LD50 and the confidence limitation of LD50 95% were calculated using Korbormethod; In oral test, the effects ofmelamine in feeds on the feed intake, growth performance, serum parameters of Japanese sea bass were investigated.The concentrations of melamine in experimental diets were 0, 500, 2000, 5000 and 10000 mg/kg respectively, each diet was fed to trip licate group s of the experimental fish, the initial body weight of Japanese sea basswas 30.58±0105g, fish were reared in a rearing system for 21d. The results showed that the solubility ofmelamine was low and the LC50 was beyond 3500 mg/L at 27 ℃ in touching test.The LD50 of Japanese sea bass in injection test was 890107mg/kg·w, and the confidence limitation of LD50 95% was 778163-1017145 mg/kg·w. In oral test, the feeding intake and growth performance were significantly decreased and the feed conversion ratio was significantly increased in 10000mg/kg melamine treatment(ppp> 0105).The 21d NOAEL(no2observed2adverse2effect2level) for melamine in Japanese sea bass was 131199 mg/kg·w·d. In conclusion, no acute toxicitywas observed formelamine solution in Japanese sea bass; the LD50 in injection testwas 890107 mg/kg·w and the confidence limitation of LD50 95% was 778163-1017145 mg/kg·w; The melamine in feed had significantly decreased the feeding rate and growth performance in Japanese sea bass, the 21d NOAEL formelamine in Japanese sea bass was 131199mg/kg·w·d.
- Melamine /
- Acute toxicity /
- Japanese sea bass

HTML全文

参考文献(16)

[2]	Shao J J,Wen J Q, Xu SW. Research p rogress on melamine toxi2cology[J]. M odern Journal of Anim al Husbandry and VeterinaryMedicine, 2007, 12: 52-54[邵静君,温家琪,徐世文. 三聚氰胺毒理学研究进展. 现代畜牧兽医. 2007, 12: 52-54]
[3]	Puschner B, Poppenga R H, Lowenstine L J, et al. Assessment ofmelamine and cyanuric acid toxicity in cats[J]. Journal of Veter2inary D iagnostic Investigation, 2007, 19(6): 616-624
[4]	Brown C A, Jeong K S, Poppenga R H, et al. Outbreaks of renalfailure associated with melamine and cyanuric acid in dogs andcats in 2004 and 2007[J]. Journal of Veterinary D iagnostic In2vestigation, 2007, 19(5): 525-531
[5]	Cianciolo R E,Bischoff K, Ebel J G, et al. Clinicopathologic, histologic, and toxicologic findings in 70 cats inadvertently exposedto pet food contaminated with melamine and cyanuric acid[J].Journal of the Am erican Veterinary m edical Association, 2008, 233(5): 729-737
[6]	Dobson R L,Motlagh S,QuijanoM, et al. Identification and char2acterization of toxicity of contaminants in pet food leading to anoutbreak of renal toxicity in cats and dogs[J]. Toxicological Sci2ences, 2008, 106(1): 251-262
[7]	Lin XM,Wang J F, J ia G L, et al. Toxicology study of melamine[J]. Journal of Toxicology, 2008, 22(3): 216-218[林祥梅,王建峰,贾广乐, 等. 三聚氰胺的毒性研究. 毒理学杂志,2008, 22(3): 216-218]
[8]	Xue M, Luo L,Wu X F, et al. Effects of six alternative lip idsources on growth and tissue fatty acid composition in Japanesesea bass(Lateolabrax japonicus)[J]. Aquaculture, 2006, 260:206-214
[9]	ChangQ,LiangM Q,Wang J L, et al. Apparent digestibility coef2ficients of various feed ingredients for Japanese Sea Bass(Lateo2labrax japonicus)[J]. Acta Hydrobiologica S inica, 2005, 29(2): 172-176[常青,梁萌青,王家林,等. 花鲈对不同饲料原料的表观消化率. 水生生物学报, 2005, 29(2): 172-176]
[10]	Luo L,Wu X F, Xue M, et al. Effect of rep lacement calciumphosphate monobasic with neutral phytase on growth and phos2phorousmetabolism of Japanese sea bass(Lateolabrax japonicus)[J]. Chinese Journal of Anim al N utrition, 2007, 19(1): 33-39[罗琳,吴秀峰,薛敏,等. 中性植酸酶在豆粕型饲料中替代磷酸二氢钙对花鲈生长及磷代谢的影响. 动物营养学报, 2007,19(1): 33-39]
[11]	Li C. Fundamental studies on resource utilization of solid wastefrom melamine manufacturing[D]. Thesis forMaster of Science.Zhengzhou University, Zhengzhou. 2000[李晨. 三聚氰胺生产过程中固体废弃物的资源化基础研究. 硕士学位论文,郑州大学,郑州. 2000]
[12]	Zhou Y X, Zhang Z S. Toxicological trialmethods of aquatic biolo2gy[M]. Beijing: Agriculture Press. 1989[周永欣,章宗涉. 水生生物毒性试验方法. 北京: 农业出版社. 1989]
[13]	IUCL ID(The International Uniform Chemical Information Data-base) Chemical Data Sheet: Substance ID 108-78-1, 2000,February 18, 30-98
[15]	US2FDA, Interim melamine and analogues safety/risk assessment.2007. US Food and Drug Administration,Washington,DC
[16]	Okumura M, Hasegawa R, Shirai T, et al. Relationship betweencalculus formation and carcinogenesis in the urinary bladder ofrats administered the non-genotoxic agents thymine or melamine[J]. Carcinogenesis, 1992, 13(6): 1043-1045
[17]	Ogasawara H, Imaida K, Ishiwata H, et al. Urinary bladder carci2nogenesis induced bymelamine in F344 male rats: correlation be2tween carcinogenicity and urolith formation[J]. Carcinogenesis,1995, 16(11): 2773-2777
[19]	Lin H R. Fish Physiology(Second Edition)[M]. Guangzhou:Guangzhou Higher Education Press. 2007[林浩然. 鱼类生理学(第二版). 广州:广东高等教育出版社. 2007]

施引文献

资源附件(0)

计量

文章访问数: 995
HTML全文浏览量: 1
PDF下载量: 796
被引次数: 0

三聚氰胺对花鲈的急性毒性实验研究

计量

出版历程

ACUTE TOXIC ITY STUDY FORMELAM INE ON JAPANESE SEA BASS(LATEOLABRAX JAPON ICUS)

计量

出版历程

目录