黄颡鱼和杂交黄颡鱼“黄优1号”形态及性腺发育的比较

胡伟华; 丹成; 郭稳杰; 樊启学; 梅洁

doi:10.7541/2019.146

黄颡鱼和杂交黄颡鱼“黄优1号”形态及性腺发育的比较

华中农业大学水产学院, 武汉 430070

基金项目: 国家现代农业产业技术体系特色淡水鱼体系项目(CARS-46)资助

详细信息

作者简介:
胡伟华(1989—), 男, 湖北黄石市人; 博士研究生; 主要从事鱼类遗传育种研究。E-mail: huweihua@webmail.hzau.edu.cn

通信作者:
梅洁, 博士, 教授; E-mail: jmei@mail.hzau.edu.cn

中图分类号: Q174
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出版历程
- 收稿日期: 2018-08-08
- 修回日期: 2019-02-28
- 网络出版日期: 2019-10-29
- 发布日期: 2019-10-31

THE MORPHOLOGY AND GONAD DEVELOPMENT OF PELTEOBAGRUS FULVIDRACO AND ITS INTERSPECIFIC HYBRID “HUANGYOU NO. 1” WITH PELTEOBAGGRUS VACHELLI

College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Funds: Supported by the China Agriculture Research System (CARS-46)

Corresponding author:

摘要

摘要: 选取相同养殖条件的10月、22月和34月龄的黄颡鱼和新品种杂交黄颡鱼(黄颡鱼P. fulvidraco♀×瓦氏黄颡鱼P. vachelli♂)“黄优1号”进行形态及性腺发育的比较研究。通过形体指标测量发现“黄优1号”生长性能显著优于黄颡鱼; 在22月和34月龄黄颡鱼中, 雄性的体重是雌性的2倍左右, 雄性生长速度显著高于雌性; 而在“黄优1号”中, 两性生长异形现象被显著减弱。基于性腺解剖形态分析发现雌性“黄优1号”卵巢完全退化, 呈细线状结构且没有卵子产生, 故“黄优1号”雌鱼完全不育; 雄性“黄优1号”精巢组织呈现透明状和退化状态。精巢组织切片HE染色分析发现10月龄“黄优1号”的精小囊为空腔状几乎没有精子产生, 22月和34月龄“黄优1号”的精小囊内出现极少量精子。计算机辅助精子分析系统(CASA)分析发现, 相比于黄颡鱼, “黄优1号”精巢中精子量非常少, 有效活力低下; 经过繁殖能力测试, 22月龄“黄优1号”雄鱼不具备繁殖能力。新品种杂交黄颡鱼“黄优1号”在生长性能提高上显现了杂交优势, 具有推动黄颡鱼产业发展的潜力。
- 黄颡鱼 /
- 瓦氏黄颡鱼 /
- 杂交黄颡鱼 /
- 性腺发育 /
- 计算机辅助精子分析系统
Abstract: The yield of hybrid yellow catfish (Pelteobagrus fulvidraco♀×Pelteobaggrus vachelli♂) was gradually increased, but the basic research is rarely. In this study, a comparative analysis of morphology and gonad development of yellow catfish (Pelteobagrus fulvidraco) and hybrid yellow catfish " Huangyou No. 1” at 3 various ages was conducted. The hybrid yellow catfish has a growth advantage to yellow catfish. 22-month and 34-month-old yellow male catfish weight twice as much as females. However, sexual size dimorphism was greatly reduced in the hybrid yellow catfish. Completely degenerated ovarian with a fine line structure and no egg production were observed in female hybrid yellow catfish, indicating that the female hybrid yellow catfish is completely sterile. Testes of male hybrid yellow catfish were transparent and degenerated. The spermatogenic cyst was almost empty and had no sperm production in 10-month-old male hybrid yellow catfish, while a few sperms were observed in the spermatogenic cyst of 22-month- and 34-month-old male hybrid yellow catfish. Compared to yellow catfish, the number of sperms of hybrid yellow catfish were very small and their activities were low based on computer assisted spermatozoa analysis system (CASA). In addition, 22-month-old male hybrid yellow catfish has no reproductive capacity. The novel variety " Huangyou No. 1” has a hybrid advantage in growth performance and is expected to promote the development of yellow catfish industry.
- Pelteobagrus fulvidraco /
- Pelteobaggrus vachelli /
- Hybrid yellow catfish /
- Gonad development /
- CASA

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图 1 黄颡鱼与“黄优1号”胸鳍前后缘的差异

Figure 1. Phenotypic differences of anterior and posterior margin of pectoral fin between yellow catfish and hybrid yellow catfish “Huangyou No. 1”

下载: 全尺寸图片幻灯片

图 2 黄颡鱼和“黄优1号”形体指标

A. 10—34月龄黄颡鱼和“黄优1号”的体长(BL)统计结果; B. 10—34月龄黄颡鱼和“黄优1号”的体重(BW)统计结果; C. 10—34月龄黄颡鱼和“黄优1号”的性腺重(GW)统计结果; D. 10—34月龄黄颡鱼和“黄优1号”的性腺成熟系数(GSI)统计结果。不同上标小写字母表示有显著性差异(P<0.05), 下同

Figure 2. The physical indicators of yellow catfish and hybrid yellow catfish “Huangyou No. 1”

A—D. Body length (BL), body weight (BW), gonad weight (GW) and gonadosomatic index (GSI) of yellow catfish and hybrid yellow catfish at age 10-34 months old. Values with different lowercase letter superscripts mean significant difference (P<0.05), the same applies bellow

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图 3 黄颡鱼和“黄优1号”性腺解剖图

A—C为10月龄、22月龄和34月龄黄颡鱼卵巢图片；D—F为10月龄、22月龄和34月龄杂交黄颡鱼卵巢图片；G—I为10月龄、22月龄和34月龄黄颡鱼精巢图片；J—L为10月龄、22月龄和34月龄杂交黄颡鱼精巢图片

Figure 3. Anatomy of the gonad of yellow catfish and hybrid yellow catfish “Huangyou No. 1”

A—C. Ovary pictures of 10-month, 22-month and 34-month old yellow catfish; D—F. Ovary pictures of 10-month, 22-month and 34-month old hybrid yellow catfish; G—I. Testis pictures of 10-month, 22-month and 34-month old yellow catfish; J—L. Testis pictures of 10-month, 22-month and 34-month old hybrid yellow catfish

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图 4 黄颡鱼和“黄优1号”精巢组织切片HE染色图

A. 10月龄黄颡鱼精巢；B—D. 10月龄、22月龄和34月龄交黄颡鱼精巢

Figure 4. The HE staining results of testis sections of yellow catfish and hybrid yellow catfish “Huangyou No. 1”

A. Testis section of 10-month old yellow catfish; B—D. Testis sections of 10-month, 22-month and 34-month old hybrid yellow catfish

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图 5 黄颡鱼和“黄优1号”1 mL体积精液的精子数量(Total)和运动精子数量(Motile)

Figure 5. The total and motile sperm per mL in yellow catfish and hybrid yellow catfish

下载: 全尺寸图片幻灯片

图 6 黄颡鱼♀×黄优1号♂(HY)和黄颡鱼♀×黄颡鱼♂(HF)胚胎发育情况

A. HY 组人工授精胚胎, 胚胎未发育至2胞期; B. HY 组人工授精胚胎, 受精后14h 受精卵变白; C. HF 组人工授精胚胎, 胚胎发育至2 胞期; D. HY 组人工授精胚胎, 受精后14h 发育至原肠胚期

Figure 6. The embryo development of yellow catfish♀×“Huangyou No. 1”♂ (HY) and yellow catfish ♀×yellow catfish♂ (HF)

A. HY embryos could not develop to 2-cell stage; B. HY embryos at 14 hpf; C. HF embryos developed to 2-cell stage; D. HF embryos developed to gastrula stage at 14 hpf

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表 1 黄颡鱼与“黄优1号”精子运动参数的检测

Table 1 The parameters of sperm motility in yellow catfish and hybrid yellow catfish (μm/s)

参数Parameter	黄颡鱼P. fulvidraco			黄优1号P. fulvidraco ♀×P. vachelli ♂
参数Parameter	10月龄 10-mouth-old	22月龄 22-mouth-old	34月龄 34-mouth-old	10月龄 10-mouth-old	22月龄 22-mouth-old	34月龄 34-mouth-old
VAP	83.12±17.91^a	87.60±6.19^a	75.38±9.05^a	18.35±4.22^b	26.71±5.11^b	27.13±5.84^b
VSL	79.12±15.88^a	80.39±5.17^a	78.16±8.44^a	16.71±4.08^b	24.33±1.31^b	24.04±6.54^b
VCL	90.42±17.40^a	97.05±7.43^a	85.44±8.45^a	21.02±5.11^b	28.48±5.61^b	29.81±7.24^b
LIN	81.37±8.29	77.51±7.12	73.28±3.33	79.44±10.26	85.67±8.25	80.32±9.23
BCF	27.43±8.83	26.34±8.64	28.01±8.09	25.73±8.01	23.01±4.09	19.94±6.89
注: 不同上标小写字母表示有显著性差异(P<0.05), 下同Note: values with different lowercase letter superscripts mean significant difference (P<0.05), the same applies bellow

下载: 导出CSV

表 2 黄颡鱼♀×“黄优1号”♂和黄颡鱼♀×黄颡鱼♂的受精率和孵化率

Table 2 Fertilization rate and hatching rate of yellow catfish♀× “Huangyou No. 1”♂ and yellow catfish♀×yellow catfish♂

	测卵数Number	受精率Fertilization rate (%)	孵化率Hatching rate (%)
黄颡鱼♀×黄优1号♂Yellow catfish♀× “Huangyou No. 1”♂	115	0	0
	120	0	0
	115	0	0
黄颡鱼♀×黄颡鱼♂yellow catfish♀×yellow catfish♂	127	71.74	90.65
	110	75.24	84.71
	128	83.15	83.87

下载: 导出CSV

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黄颡鱼和杂交黄颡鱼“黄优1号”形态及性腺发育的比较

作者简介: 胡伟华(1989—), 男, 湖北黄石市人; 博士研究生; 主要从事鱼类遗传育种研究。E-mail: huweihua@webmail.hzau.edu.cn

通信作者: 梅洁, 博士, 教授; E-mail: jmei@mail.hzau.edu.cn

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THE MORPHOLOGY AND GONAD DEVELOPMENT OF PELTEOBAGRUS FULVIDRACO AND ITS INTERSPECIFIC HYBRID “HUANGYOU NO. 1” WITH PELTEOBAGGRUS VACHELLI

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作者简介:
胡伟华(1989—), 男, 湖北黄石市人; 博士研究生; 主要从事鱼类遗传育种研究。E-mail: huweihua@webmail.hzau.edu.cn

通信作者:
梅洁, 博士, 教授; E-mail: jmei@mail.hzau.edu.cn