Volume 42 Issue 4
Aug.  2018
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TENG Shuang-Shuang, FANG Jun, SHAO Yan-Qing, LIN Xing-Guan, CHAI Xue-Liang, XIAO Guo-Qiang. MICROSATELLITE ANALYSIS OF GENETIC VARIATION IN THE FAST GROWTH FAMILIES OF THE 2nd GENERATION OF TEGILLARCA GRANOSA AND CORRELATION WITH GROWTH TRAITS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 681-689. DOI: 10.7541/2018.084
Citation: TENG Shuang-Shuang, FANG Jun, SHAO Yan-Qing, LIN Xing-Guan, CHAI Xue-Liang, XIAO Guo-Qiang. MICROSATELLITE ANALYSIS OF GENETIC VARIATION IN THE FAST GROWTH FAMILIES OF THE 2nd GENERATION OF TEGILLARCA GRANOSA AND CORRELATION WITH GROWTH TRAITS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 681-689. DOI: 10.7541/2018.084
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MICROSATELLITE ANALYSIS OF GENETIC VARIATION IN THE FAST GROWTH FAMILIES OF THE 2nd GENERATION OF TEGILLARCA GRANOSA AND CORRELATION WITH GROWTH TRAITS

  • 1.

    Zhejiang Mariculture Research Institute, Wenzhou 325005, China

  • 2.

    Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou 325005, China

  • 3.

    Engineering Research Center for Marine Bivalves, Chinese Academy of Fishery Sciences, Wenzhou 325005, China

Funds: Supported by the Earmarked Fund for Modern Argo-Industry Technology Research System (CARS-47); Seed Science and Technology Innovation Project ofWenzhou City (N20120017); Science and Technology Planning Project of Zhejiang Province (2017F30042, 2018F10015)
  • Received Date: May 14, 2017
  • Rev Recd Date: March 13, 2018
  • Available Online: May 16, 2018
  • Published Date: June 30, 2018
    Graphical Abstract
    • Abstract
  • In this study, microsatellite markers were used to assess the genetic structure and genetic diversity of the second generation of Tegillarca granosa. In addition, the correlation of these markers with growth-related traits was assessed to explore their potential use for marker-assisted breeding. Genome-wide identification and structural analysis herein led to detect 59 alleles through 18 pairs of primers. Among these, the average values of alleles (Na) in the families of F19, F21, and F22 were 2.500, 2.722, and 2.722, respectively. The average observed heterozygosity (Ho) was 0.446, 0.510, and 0.628; the average expected heterozygosity (He) was 0.394, 0.433, and 0.464, respectively. In addition, the corresponding values of polymorphism information content (PIC) were 0.346, 0.379, and 0.403, respectively. Correlation analyses of these polymorphic markers and growth traits revealed significant association of three loci with shell height, shell length, shell width, and total weight. Specifically, genotype BB of Teg-30 in F19 was remarkably associated with shell height and total weight, while genotype BB of Teg-03 and genotype BC of Teg-20 in F21 showed a significant correlation with the shell height, shell length, shell width, and total weight. As a result, the growth-related markers, identified in the present study, may provide precious genetic information and novel insights for molecular marker-assisted breeding of T. granosa.
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