IDENTIFICATION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED MIRNA UNDER LOW TEMPERATURE AND VALIDATION OF TARGET GENES IN <i>LITOPENAEUS VANNAMEI</i>

ZHUO Xiao-Fei; HE Ping-Ping; WEI Pin-Yuan; CHEN Xiao-Han; PENG Jin-Xia

doi:10.7541/2019.142

Volume 43 Issue 6

Nov. 2019

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ACTA HYDROBIOLOGICA SINICA > 2019 > 43(6): 1203-1209. > DOI: 10.7541/2019.142

ZHUO Xiao-Fei, HE Ping-Ping, WEI Pin-Yuan, CHEN Xiao-Han, PENG Jin-Xia. IDENTIFICATION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED MIRNA UNDER LOW TEMPERATURE AND VALIDATION OF TARGET GENES IN LITOPENAEUS VANNAMEI[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(6): 1203-1209. DOI: 10.7541/2019.142

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IDENTIFICATION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED MIRNA UNDER LOW TEMPERATURE AND VALIDATION OF TARGET GENES IN LITOPENAEUS VANNAMEI

1.
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
2.
Guangxi Academy of Fisheries Science, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning 530021, China

Funds: Supported by the Special Funds for Ba Gui Scholars Project Construction (BGXZ-NMBDX-05); the Natural Science Foundation of Guangxi Zhuang Autonomous Region (2017 GXNSFBA198048); the Natural Science Foundation of China (31460690) and the Construction of Modern Agricultural Industry Technology System (CARS-47)

Received Date: December 18, 2018
Rev Recd Date: July 07, 2019
Available Online: October 29, 2019
Published Date: October 31, 2019

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Abstract

Abstract

To examine the molecular regulatory mechanism of microRNAs and target genes in Litopenaeus vannamei during cold adaptation, we conducted microRNAs (miRNAs) analysis on the hepatopancreas of L. vannamei under normal temperature of 28℃ and cold acclimation (16℃ for 6 days) by using Solexa sequencing. In total, 10690259 and 8587144 unique sequences of 18—32 nt length were obtained from small RNA libraries at room temperature and low temperature, including 57 and 48 known mature miRNAs, respectively. Expression analysis revealed 25 significantly differential expressed miRNAs between groups, and three of them were further confirmed by PCR. Moreover, we also observed that cold regulated target genes of significantly differential expressed miRNAs including the nuclear export mediator factor Nemf-lik, synapse-associated protein, seleno proteins and DEAD-box RNA helicase Variant 1. These results suggest that miRNAs and their target genes may mediate the cold adaption in L. vannamei.
- Litopenaeus vannamei,
- Cold stress,
- miRNA,
- Target genes,
- qRT-PCR

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IDENTIFICATION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED MIRNA UNDER LOW TEMPERATURE AND VALIDATION OF TARGET GENES IN LITOPENAEUS VANNAMEI

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