CHARACTERISTICS OF <i>MTF-1</i> AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH <i>SINIPERCA CHUATSI</i>

PAN Ya-Xiong; ZHOU Jun; ZHANG Yu; TAO Jin-Sheng; PAN Jia-Lin; TANG Zhao-Yang; FAN Yi-Wei; HU Ming-Guang; LI Hui-Ju; ZHANG Jian-She; CHU Wu-Ying

doi:10.7541/2023.2022.0131

To study the characteristics of mtf-1 gene of Siniperca chuatsi and the effects of cadmium stress on the circadian rhythm of mtf-1 gene in brain, we analyzed cis-regulatory elements of mtf-1 gene promoter, sequence characteristics and tissues expression patterns of mtf-1 gene, and explored the circadian rhythm changes of mtf-1 gene expression in brain of Siniperca chuatsi under cadmium stress. The results showed that there were binding sites of zinc finger proteins including ZNF136, ZNF384 and ZNF143 in the mtf-1 promoter of S. chuatsi, indicating that their expression is potentially regulated by metal ions. In addition, RORβ binding sites of the core clock gene were also found in the mtf-1 promoter of S. chuatsi, indicating that the mtf-1 gene is potentially involved in circadian rhythm regulation. The analysis of function domain indicated that mtf-1 gene has a highly conserved DNA binding domain and a non-conserved trans-activation domain. mtf-1 gene is expressed in different tissues of S. chuatsi, among which the highest expression level is found in brain tissue, followed by kidney. Under natural conditions, mtf-1 gene showed a trend of high expression at night and low expression in the brain at day. The expression peak phase occurs at ZT 12.65h (nightfall), which is consistent with its life habits. Under cadmium stress, the mtf-1 gene in the brain of S. chuatsi presented continuous low expression level, and with no significant difference between day and night, indicating that the mtf-1 gene expression in the brain of S. chuatsi could be significantly inhibited by cadmium, and the circadian rhythm of mtf-1 in the brain tissue of S. chuatsi is damaged.

[1]	Chen G H, Lv W, Xu Y H, et al. Functional analysis of MTF-1 and MT promoters and their transcriptional response to zinc (Zn) and copper (Cu) in yellow catfish Pelteobagrus fulvidraco [J]. Chemosphere, 2020(246): 125792.
[2]	Chen S W, Wu K, Lv W H, et al. Functional analysis of two zinc (Zn) transporters (ZIP3 and ZIP8) promoters and their distinct response to MTF1 and RREB1 in the regulation of Zn metabolism [J]. International Journal of Molecular Sciences, 2020, 21(17): 6135. doi: 10.3390/ijms21176135
[3]	Jackson A C, Liu J, Vallanat B, et al. Identification of novel activators of the metal responsive transcription factor (MTF-1) using a gene expression biomarker in a microarray compendium [J]. Metallomics, 2020, 12(9): 1400-1415. doi: 10.1039/d0mt00071j
[4]	Talukder M, Bi S S, Jin H T, et al. Cadmium induced cerebral toxicity via modulating MTF1-MTs regulatory axis [J]. Environmental Pollution, 2021(285): 117083.
[5]	Günther V, Lindert U, Schaffner W. The taste of heavy metals: Gene regulation by MTF-1 [J]. Biochimica et Biophysica Acta (BBA)- Molecular Cell Research, 2012, 1823(9): 1416-1425. doi: 10.1016/j.bbamcr.2012.01.005
[6]	Radtke F, Georgiev O, Müller H P, et al. Functional domains of the heavy metal-responsive transcription regulator MTF-1 [J]. Nucleic Acids Research, 1995, 23(12): 2277-2286. doi: 10.1093/nar/23.12.2277
[7]	Li Y, Kimura T, Laity J H, et al. The zinc-sensing mechanism of mouse MTF-1 involves linker peptides between the zinc fingers [J]. Molecular and Cellular Biology, 2006, 26(15): 5580-5587. doi: 10.1128/MCB.00471-06
[8]	Radtke F, Heuchel R, Georgiev O, et al. Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter [J]. The EMBO Journal, 1993, 12(4): 1355-1362. doi: 10.1002/j.1460-2075.1993.tb05780.x
[9]	Wimmer U, Wang Y, Georgiev O, et al. Two major branches of anti-cadmium defense in the mouse: MTF-1/metallothioneins and glutathione [J]. Nucleic Acids Research, 2005, 33(18): 5715-5727. doi: 10.1093/nar/gki881
[10]	Chen W Y, John J A C, Lin C H, et al. Expression pattern of metallothionein, MTF-1 nuclear translocation, and its dna-binding activity in zebrafish (Danio rerio) induced by zinc and cadmium [J]. Environmental Toxicology and Chemistry, 2007, 26(1): 110-117. doi: 10.1897/06-153R.1
[11]	Ferencz Á, Hermesz E. Identification and characterization of two mtf-1 genes in common carp [J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2008, 148(3): 238-243.
[12]	Pan Y X, Luo Z, Zhuo M Q, et al. Oxidative stress and mitochondrial dysfunction mediated Cd-induced hepatic lipid accumulation in zebrafish Danio rerio [J]. Aquatic Toxicology, 2018(199): 12-20.
[13]	Kim Y, Park H R, Yeo W J, et al. The correlation between bioaccumulation and pattern of stress-related genes expression of black sea bream (Acanthopagrus schlegeli) by cadmium exposure [J]. Ocean Science Journal, 2017, 52(2): 231-242. doi: 10.1007/s12601-017-0022-7
[14]	Jiménez-Ortega V, Cardinali D P, Fernández-Mateos M P, et al. Effect of cadmium on 24-hour pattern in expression of redox enzyme and clock genes in rat medial basal hypothalamus [J]. Biometals, 2010, 23(2): 327-337. doi: 10.1007/s10534-010-9292-6
[15]	Jiménez Ortega V, Cano-Barquilla P, Scacchi P A, et al. Cadmium-induced disruption in 24-h expression of clock and redox enzyme genes in rat medial basal hypothalamus: prevention by melatonin [J]. Frontiers in Neurology, 2011(2): 13.
[16]	Jiménez-Ortega V, Cano Barquilla P, Fernández-Mateos P, et al. Cadmium as an endocrine disruptor: correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [J]. Free Radical Biology and Medicine, 2012, 53(12): 2287-2297. doi: 10.1016/j.freeradbiomed.2012.10.533
[17]	Wu P, Li Y L, Cheng J, et al. Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi) [J]. BMC Genomics, 2016, 17(1): 1008. doi: 10.1186/s12864-016-3373-z
[18]	张瑞祺, 郝月月, 宋银都, 等. 鳜视觉和侧线感觉调控捕食行为的动态观察 [J]. 中国水产科学, 2020, 27(10): 1136-1144. Zhang R Q, Hao Y Y, Song Y D, et al. Predation behavior of mandarin fish (Siniperca chuatsi) regulated by visual and lateral line sensory [J]. Journal of Fishery Sciences of China, 2020, 27(10): 1136-1144.
[19]	Hongyan W, Jinliang Z, Shoujie T, et al. Pollution status and risk analysis on heavy metals in muscles of Siniperca chuatsi from East China [J]. Asian Journal of Ecotoxicology, 2020(2): 268-278.
[20]	刘晶洁, 褚武英, 朱鑫, 等. 鳜RORα基因的胚胎发育特征及饥饿对其节律性表达影响分析 [J]. 广西师范大学学报(自然科学版), 2021, 39(5): 190-197. Liu J J, Chu W Y, Zhu X, et al. Embryonic development characteristics and rhythmic expression analysis of RORα gene under starvation in Siniperca chuatsi [J]. Journal of Guangxi Normal University (Natural Science Edition), 2021, 39(5): 190-197.
[21]	陈圆华, 李虹辉, 王利香, 等. GSK4112对尼罗罗非鱼肝脏Nr1d1和Ulk1b基因昼夜节律性表达的影响 [J]. 基因组学与应用生物学, 2021, 40(2): 584-590. Chen Y H, Li H H, Wang L X, et al. Effects of GSK4112 on circadian rhythmicity expression of Nr1d1 and Ulk1b in liver of Nile tilapia (Oreochromis niloticus) [J]. Genomics and Applied Biology, 2021, 40(2): 584-590.
[22]	Chen W Y, John J A C, Lin C H, et al. Molecular cloning and developmental expression of zinc finger transcription factor MTF-1 gene in zebrafish Danio rerio [J]. Biochemical and Biophysical Research Communications, 2002, 291(4): 798-805. doi: 10.1006/bbrc.2002.6517
[23]	Christensen K A, Davidson W S. Autopolyploidy genome duplication preserves other ancient genome duplications in Atlantic salmon (Salmo salar) [J]. PLoS One, 2017, 12(2): e0173053. doi: 10.1371/journal.pone.0173053
[24]	Li J T, Hou G Y, Kong X F, et al. The fate of recent duplicated genes following a fourth-round whole genome duplication in a tetraploid fish, common carp (Cyprinus carpio) [J]. Scientific Reports, 2015, 5(1): 1-9. doi: 10.9734/JSRR/2015/14076
[25]	Lichtlen P, Georgiev O, Schaffner W, et al. The heavy metal-responsive transcription factor-1 (MTF-1) is not required for neural differentiation [J]. Biological chemistry, 1999, 380(6): 711-715.
[26]	Günes Ç, Heuchel R, Georgiev O, et al. Embryonic lethality and liver degeneration in mice lacking the metal-responsive transcriptional activator MTF-1 [J]. The EMBO Journal, 1998, 17(10): 2846-2854. doi: 10.1093/emboj/17.10.2846
[27]	Lichtlen P, Schaffner W. Putting its fingers on stressful situations: the heavy metal-regulatory transcription factor MTF-1 [J]. Bioessays, 2001, 23(11): 1010-1017. doi: 10.1002/bies.1146
[28]	André E, Conquet F, Steinmayr M, et al. Disruption of retinoid-related orphan receptor β changes circadian behavior, causes retinal degeneration and leads to vacillans phenotype in mice [J]. The EMBO Journal, 1998, 17(14): 3867-3877. doi: 10.1093/emboj/17.14.3867
[29]	Cassandri M, Smirnov A, Novelli F, et al. Zinc-finger proteins in health and disease [J]. Cell Death Discovery, 2017, 3(1): 1-12.
[30]	Lee S Y, Nam Y K. Molecular cloning of metal-responsive transcription factor-1 (MTF-1) and transcriptional responses to metal and heat stresses in Pacific abalone, Haliotis discus hannai [J]. Fisheries and Aquatic Sciences, 2017, 20(1): 1-13. doi: 10.1186/s41240-017-0046-z
[31]	Lazado C C, Skov P V, Pedersen P B. Innate immune defenses exhibit circadian rhythmicity and differential temporal sensitivity to a bacterial endotoxin in Nile tilapia (Oreochromis niloticus) [J]. Fish & Shellfish Immunology, 2016(55): 613-622.
[32]	Cuypers A, Plusquin M, Remans T, et al. Cadmium stress: an oxidative challenge [J]. Biometals, 2010, 23(5): 927-940. doi: 10.1007/s10534-010-9329-x
[33]	Patra R C, Rautray A K, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration [J]. Veterinary Medicine International, 2011(2011): 457327.
[34]	Lafuente A. The hypothalamic–pituitary–gonadal axis is target of cadmium toxicity. An update of recent studies and potential therapeutic approaches [J]. Food and Chemical Toxicology, 2013(59): 395-404.
[35]	Xiao B, Chen T M, Zhong Y. Possible molecular mechanism underlying cadmium-induced circadian rhythms disruption in zebrafish [J]. Biochemical and Biophysical Research Communications, 2016, 481(3-4): 201-205. doi: 10.1016/j.bbrc.2016.10.081
[36]	Zheng J L, Yuan S S, Wu C W, et al. Acute exposure to waterborne cadmium induced oxidative stress and immunotoxicity in the brain, ovary and liver of zebrafish (Danio rerio) [J]. Aquatic Toxicology, 2016(180): 36-44.
[37]	Oishi K, Miyazaki K, Kadota K, et al. Genome-wide expression analysis of mouse liver reveals CLOCK-regulated circadian output genes [J]. Journal of Biological Chemistry, 2003, 278(42): 41519-41527. doi: 10.1074/jbc.M304564200
[38]	Reinke H, Asher G. Circadian clock control of liver metabolic functions [J]. Gastroenterology, 2016, 150(3): 574-580. doi: 10.1053/j.gastro.2015.11.043

CHARACTERISTICS OF MTF-1 AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH SINIPERCA CHUATSI

Abstract

References

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Related

CHARACTERISTICS OF MTF-1 AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH SINIPERCA CHUATSI

Abstract

References

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Related

Export File

Citation

Format

Content