| Citation: |
XIU Wen-Jie, YAN Miao, GU Ji-Hai, LIU Feng-Song, ZHANG Yu-Ming. ACUTE AND CHRONIC TOXICOLOGICAL EFFECTS ON COMBINED EXPOSURE OF MICROPLASTIC PVC AND SULFAMETHOXAZOLE TO DAPHNIA MAGNA[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(3): 413-425. DOI: 10.7541/2024.2023.0166
|
With the increasing production and application of plastics, there is growing concern regarding the ubiquitous distribution of microplastics and their coexistence with various contaminants. Meanwhile, antibiotics have been ubiquitously detected in aquatic environments due to their extensive production, poor absorption, and improper disposal of unused drugs. Sulfamethoxazole (SMZ), commonly used in veterinary medicine and aquaculture as an antibacterial compound, has been found in aquatic environments frequently. Interestingly, SMZ is also used as a feed additive to promote animal growth in farms and fish aquaculture. Polyvinyl chloride (PVC) microplastics, one of the most commonly used plastic materials, have been detected in various environmental compartments, including sediment, surface water, marine, and freshwater. Despite the toxicity of PVC and SMZ has been extensively studied, the combined toxic effects induced by the two pollutants on aquatic organisms remain largely unknown. In this study, adsorption experiments between PVC and SMZ were conducted. Then, Daphnia magna, a commonly used aquatic crustacean in ecotoxicological assessments, was chosen as the model organism to investigate the joint toxic effects of PVC and SMZ through 48h acute and 21d chronic toxicity experiments. We assessed a chain of parameters, including survival, growth, reproduction, intestinal structure, and locomotor behavior. The results showed that PVC could absorb SMZ through physical interaction. In the acute toxicity experiments, the presence of PVC (1 mg/L) reduced the SMZ content in D. magna individuals, thereby attenuating the lethal effects induced by SMZ exposure. In the chronic toxicity experiments, exposure by SMZ alone or in combination with PVC (1 mg/L) showed no significant effect on the mortality ratio, number of molts, or body length of the D. magna. However, the exposure to individuals of D. magna by SMZ alone or in combination with PVC aroused oxidative stress, intestinal damage, and locomotor inhibition. Moreover, the presence of PVC exacerbated the toxic effects. In conclusion, the findings revealed both antagonistic and synergistic toxic effects of PVC on SMZ, depending on the exposure dose and time. The chronic assays (21d) performed with the mixture of environmentally relevant concentrations of contaminants indicated that PVC enhanced the toxicity of SMZ to D. magna. This study provides new insights into the ecotoxicological effects caused by the co-existence of PVC and SMZ in aquatic environments. The study can also convince a reference for the underlying mechanisms of environmental behavior and toxicity aroused by the coexistence of microplastics and antibiotics on aquatic organisms.
| [1] |
武芳竹, 曾江宁, 徐晓群, 等. 海洋微塑料污染现状及其对鱼类的生态毒理效应 [J]. 海洋学报, 2019, 41(2): 85-98.
Wu F Z, Zeng J N, Xu X Q, et al. Status of marine microplastic pollution and its ecotoxicological effects on marine fish [J]. Acta Oceanologica Sinica, 2019, 41(2): 85-98.
|
| [2] |
Li J Y, Liu H H, Paul C J. Microplastics in freshwater systems: a review on occurrence, environmental effects, and methods for microplastics detection [J]. Water Research, 2018(137): 362-374.
|
| [3] |
Yu F, Li Y, Huang G Q, et al. Adsorption behavior of the antibiotic levofloxacin on microplastics in the presence of different heavy metals in an aqueous solution [J]. Chemosphere, 2020(260): 127650.
|
| [4] |
Hoseini S M, Khosraviani K, Hosseinpour-Delavar F, et al. Hepatic transcriptomic and histopathological responses of common carp, Cyprinus carpio, to copper and microplastic exposure [J]. Marine Pollution Bulletin, 2022(175): 113401.
|
| [5] |
盛诚, 王馨颖, 易珍, 等. 不同材质微塑料对三氯生的吸附及其在斑马鱼体内累积分布的影响 [J]. 生态毒理学报, 2020, 15(5): 108-117.
Sheng C, Wang X Y, Yi Z, et al. The adsorption of tricolsan by microplastics of different polymers and its effect on tricolsan accumulation in zebrafish [J]. Asian Journal of Ecotoxicology, 2020, 15(5): 108-117.
|
| [6] |
Karami A, Romano N, Galloway T, et al. Virgin microplastics cause toxicity and modulate the impacts of phenanthrene on biomarker responses in African catfish (Clarias gariepinus) [J]. Environmental Research, 2016(151): 58-70.
|
| [7] |
Wang M J, Wang W X. Accumulation kinetics and gut microenvironment responses to environmentally relevant doses of micro/nanoplastics by zooplankton Daphnia magna [J]. Environmental Science & Technology, 2023, 57(14): 5611-5620.
|
| [8] |
张涛, 刘凯, 孙一鑫, 等. 微塑料对双齿围沙蚕生理代谢的影响研究 [J]. 生态毒理学报, 2021, 16(4): 271-279.
Zhang T, Liu K, Sun Y X, et al. Effects of microplastics on physiological metabolism of Perinereis aibuhitensis [J]. Asian Journal of Ecotoxicology, 2021, 16(4): 271-279.
|
| [9] |
J]. Acta Scientiae Circumstantiae, 2021, 41(4): 1538-1544. [王琳, 马旖旎, 季荣. PS和PVC微塑料对小球藻的生长抑制效应及其影响途径 [J]. 环境科学学报, 2021, 41(4): 1538-1544.
Wang L, Ma Y N, Ji R. Effects of PS and PVC microplastics on the growth of Chlorella sp
|
| [10] |
张国栋, 董文平, 刘晓晖, 等. 我国水环境中抗生素赋存、归趋及风险评估研究进展 [J]. 环境化学, 2018, 37(7): 1491-1500.
Zhang G D, Dong W P, Liu X H, et al. Occurrence, fate and risk assessment of antibiotics in water environment of China [J]. Environmental Chemistry, 2018, 37(7): 1491-1500.
|
| [11] |
徐鑫磊, 刘建超, 陆光华. 8种典型PhACs在水中的赋存、生态风险及其对大型溞的影响 [J]. 环境科学, 2020, 41(5): 2239-2246.
Xu X L, Liu J C, Lu G H. Occurrence and ecological risk of eight typical PhACs in surface water and its impact on Daphnia magna [J]. Environmental Science, 2020, 41(5): 2239-2246.
|
| [12] |
Zhang Y M, Xiu W J, Yan M, et al. Adverse effects of sulfamethoxazole on locomotor behavior and lipid metabolism by inhibiting acetylcholinesterase and lipase in Daphnia magna [J]. Science of the Total Environment, 2023(892): 164631.
|
| [13] |
Nie X P, Liu B Y, Yu H J, et al. Toxic effects of erythromycin, ciprofloxacin and sulfamethoxazole exposure to the antioxidant system in Pseudokirchneriella subcapitata [J]. Environmental Pollution, 2013(172): 23-32.
|
| [14] |
Yu K, Qiu Y S, Shi Y, et al. Association of long-term effects of low-level sulfamethoxazole with ovarian lipid and amino acid metabolism, sex hormone levels, and oocyte maturity in zebrafish [J]. Ecotoxicology and Environmental Safety, 2022(247): 114234.
|
| [15] |
Limbu S M, Zhou L, Sun S X, et al. Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk [J]. Environment International, 2018(115): 205-219.
|
| [16] |
Zhao H J, Wang Y, Guo M H, et al. Environmentally relevant concentration of cypermethrin or/and sulfamethoxazole induce neurotoxicity of grass carp: involvement of blood-brain barrier, oxidative stress and apoptosis [J]. Science of the Total Environment, 2021(762): 143054.
|
| [17] |
Serra-Compte A, Sánchez-Melsió Á, Álvarez-Muñoz D, et al. Exposure to a subinhibitory sulfonamide concentration promotes the spread of antibiotic resistance in marine blue mussels (Mytilus edulis) [J]. Environmental Science & Technology Letters, 2019, 6(4): 211-215.
|
| [18] |
Wang Y X, Liu M J, Geng X H, et al. The combined effects of microplastics and the heavy metal cadmium on the marine periphytic ciliate Euplotes vannus [J]. Environmental Pollution, 2022(308): 119663.
|
| [19] |
Li J, Li J, Zhai L, et al. Co-exposure of polycarbonate microplastics aggravated the toxic effects of imidacloprid on the liver and gut microbiota in mice [J]. Environmental Toxicology and Pharmacology, 2023(101): 104194.
|
| [20] |
Oliveira-Pereira E A, Labine L M, Kleywegt S, et al. Daphnia magna sub-lethal exposure to phthalate pollutants elicits disruptions in amino acid and energy metabolism [J]. Aquatic Toxicology, 2023(257): 106432.
|
| [21] |
龚庆, 于涛, 徐闻欣, 等. MoS2强化Fe2+活化单过硫酸盐去除磺胺甲恶唑和还原六价铬的研究 [J]. 环境科学学报, 2021, 41(4): 1351-1358.
Gong Q, Yu T, Xu W X, et al. Improved removal of sulfamethoxazole and Cr (Ⅵ) by the addition of MoS2 into the Fe2+/PMS process [J]. Acta Scientiae Circumstantiae, 2021, 41(4): 1351-1358.
|
| [22] |
范秀磊, 邹晔锋, 刘加强, 等. 老化前后轮胎磨损微粒与聚氯乙烯微粒对抗生素的吸附-解吸行为 [J]. 环境科学, 2021, 42(4): 1901-1912. doi: 10.13227/j.hjkx.202008179
Fan X L, Zou Y F, Liu J Q, et al. Adsorption and desorption behaviors of antibiotics on TWP and PVC particles before and after aging [J]. Environmental Science, 2021, 42(4): 1901-1912. doi: 10.13227/j.hjkx.202008179
|
| [23] |
OECD. Test No. 202: Daphnia sp. Acute Immobilisation Test [M]. Paris: OECD Publishing, 2004: 1-12.
|
| [24] |
Broderius S J, Kahl M D, Hoglund M D. Use of joint toxic response to define the primary mode of toxic action for diverse industrial organic chemicals [J]. Environmental Toxicology and Chemistry, 1995, 14(9): 1591-1605. doi: 10.1002/etc.5620140920
|
| [25] |
Na J, Song J, Achar J C, et al. Synergistic effect of microplastic fragments and benzophenone-3 additives on lethal and sublethal Daphnia magna toxicity [J]. Journal of Hazardous Materials, 2021(402): 123845.
|
| [26] |
Li Z C, Zhou H, Liu Y, et al. Acute and chronic combined effect of polystyrene microplastics and dibutyl phthalate on the marine copepod Tigriopus japonicus [J]. Chemosphere, 2020(261): 127711.
|
| [27] |
Gu J H, Lin D D, Sun Y Y, et al. Integrating transcriptome and physiological analysis to reveal the essential responses of Daphnia magna to antimony trioxide nanoparticle [J]. Journal of Hazardous Materials, 2022(437): 129303.
|
| [28] |
Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry, 1976, 72(1/2): 248-254.
|
| [29] |
Tran V S, Ngo H H, Guo W, et al. New chitosan-biochar composite derived from agricultural waste for removing sulfamethoxazole antibiotics in water [J]. Bioresource Technology, 2023(385): 129384.
|
| [30] |
Qin L T, Pang X R, Zeng H H, et al. Ecological and human health risk of sulfonamides in surface water and groundwater of Huixian karst wetland in Guilin, China [J]. Science of the Total Environment, 2020(708): 134552.
|
| [31] |
Yan M, Nie H Y, Xu K H, et al. Microplastic abundance, distribution and composition in the Pearl River along Guangzhou city and Pearl River estuary, China [J]. Chemosphere, 2019(217): 879-886.
|
| [32] |
Liu Y, Zhang J L, Zhao H Y, et al. Effects of polyvinyl chloride microplastics on reproduction, oxidative stress and reproduction and detoxification-related genes in Daphnia magna [J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2022(254): 109269.
|
| [33] |
Xiao B H, Wang J J, Liao B L, et al. Combined effects of copper and microplastics on physiological parameters of Tubastrea aurea corals [J]. Environmental Science and Pollution Research, 2022, 29(10): 14393-14399. doi: 10.1007/s11356-021-16665-6
|
| [34] |
刘迪, 童非, 高岩, 等. 重金属存在下微塑料对环丙沙星的吸附特征及机制研究 [J]. 农业环境科学学报, 2021, 40(5): 1017-1025. doi: 10.11654/jaes.2020-1350
Liu D, Tong F, Gao Y, et al. The characteristics and mechanisms of microplastic adsorption by ciprofloxacin in the presence of heavy metals [J]. Journal of Agro-Environment Science, 2021, 40(5): 1017-1025. doi: 10.11654/jaes.2020-1350
|
| [35] |
Lin L J, Tang S, Wang X S, et al. Accumulation mechanism of tetracycline hydrochloride from aqueous solutions by nylon microplastics [J]. Environmental Technology & Innovation, 2020(18): 100750.
|
| [36] |
林陆健, 汤帅, 孙璇, 等. 铅离子和四环素在微塑料表面的吸附机理与协同效应 [J]. 环境科学学报, 2021, 41(10): 4022-4031. doi: 10.13671/j.hjkxxb.2021.0052
Lin L J, Tang S, Sun X, et al. Adsorption of Pb (Ⅱ) ions and tetracycline onto microplastics: interaction mechanisms and synergistic effects [J]. Acta Scientiae Circumstantiae, 2021, 41(10): 4022-4031. doi: 10.13671/j.hjkxxb.2021.0052
|
| [37] |
Liu G Z, Zhu Z L, Yang Y X, et al. Sorption behavior and mechanism of hydrophilic organic chemicals to virgin and aged microplastics in freshwater and seawater [J]. Environmental Pollution, 2019(246): 26-33.
|
| [38] |
Xu B, Liu F, Brookes P C, et al. The sorption kinetics and isotherms of sulfamethoxazole with polyethylene microplastics [J]. Marine Pollution Bulletin, 2018(131): 191-196.
|
| [39] |
Li J, Zhang K, Zhang H. Adsorption of antibiotics on microplastics [J]. Environmental Pollution, 2018(237): 460-467.
|
| [40] |
张石云, 宋超, 张敬卫, 等. 微塑料暴露对罗非鱼肌肉中磺胺甲唑残留的影响 [J]. 生态与农村环境学报, 2018, 34(9): 857-864.
Zhang S Y, Song C, Zhang J W, et al. Effects of micro-plastics on sulfamethoxazole (SMZ) residues in Tilapia (Oreochromis niloticus) muscle [J]. Journal of Ecology and Rural Environment, 2018, 34(9): 857-864.
|
| [41] |
Liu J, Lv M, Sun A Q, et al. Exposure to microplastics reduces the bioaccumulation of sulfamethoxazole but enhances its effects on gut microbiota and the antibiotic resistome of mice [J]. Chemosphere, 2022(294): 133810.
|
| [42] |
李勤, 李尚谕, 熊雄, 等. 微塑料对大型溞的急性毒性研究 [J]. 水生生物学报, 2021, 45(2): 292-298. doi: 10.7541/2021.2019.180
Li Q, Li S Y, Xiong X, et al. Study on acute toxicity of microplastic to Daphnia magna [J]. Acta Hydrobiologica Sinica, 2021, 45(2): 292-298. doi: 10.7541/2021.2019.180
|
| [43] |
何水清. 微塑料与磺胺类抗生素对海水青鳉肠道微生物与生殖健康的复合效应研究 [D]. 福州: 福建师范大学, 2020: 37-49.
He S Q. Combined toxicities of microplastics and sulfonamide on the intestinal microorganisms and reproduction in marine medaka [D]. Fuzhou: Fujian Normal University, 2020: 37-49.
|
| [44] |
Bownik A. Physiological endpoints in daphnid acute toxicity tests. [J] Science of the Total Environment, 2020(700): 134400.
|
| [45] |
Zhang Y M, Li Y J, Feng Q, et al. Polydatin attenuates cadmium-induced oxidative stress via stimulating SOD activity and regulating mitochondrial function in Musca domestica larvae [J]. Chemosphere, 2020(248): 126009.
|
| [46] |
Wang Y, Branicky R, Noë A, et al. Superoxide dismutases: dual roles in controlling ROS damage and regulating ROS signaling [J]. Journal of Cell Biology, 2018, 217(6): 1915-1928. doi: 10.1083/jcb.201708007
|
| [47] |
Hu F, Ye K, Tu X F, et al. Identification and expression profiles of twenty-six glutathione S-transferase genes from rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) [J]. International Journal of Biological Macromolecules, 2018(120): 1063-1071.
|
| [48] |
方海燕, 谢冰寒, 侯淼淼, 等. PVC微塑料对大型溞繁殖及应激与能量相关基因表达的影响 [J]. 环境科学学报, 2021, 41(5): 2056-2062. doi: 10.13671/j.hjkxxb.2020.0428
Fang H Y, Xie B H, Hou M M, et al. Effect of PVC microplastic on reproduction and energy-related gene expression of Daphnia magna [J]. Acta Scientiae Circumstantiae, 2021, 41(5): 2056-2062. doi: 10.13671/j.hjkxxb.2020.0428
|
| [49] |
De-Liguoro M, Riga A, Fariselli P. Synergistic toxicity of some sulfonamide mixtures on Daphnia magna [J]. Ecotoxicology and Environmental Safety, 2018(164): 84-91.
|
| [50] |
Meng X L, Hu W P, Wu S K, et al. Chinese yam peel enhances the immunity of the common carp (Cyprinus carpio L.) by improving the gut defence barrier and modulating the intestinal microflora [J]. Fish & Shellfish Immunology, 2019(95): 528-537.
|
| [51] |
Wang Y, Mao Z, Zhang M X, et al. The uptake and elimination of polystyrene microplastics by the brine shrimp, Artemia parthenogenetica, and its impact on its feeding behavior and intestinal histology [J]. Chemosphere, 2019(234): 123-131.
|
| [52] |
Ahrendt C, Perez-Venegas D J, Urbina M, et al. Microplastic ingestion cause intestinal lesions in the intertidal fish Girella laevifrons [J]. Marine Pollution Bulletin, 2020(151): 110795.
|
| [53] |
Chen Q Q, Lackmann C, Wang W, et al. Microplastics lead to hyperactive swimming behaviour in adult zebrafish [J]. Aquatic Toxicology, 2020(224): 105521.
|
| [54] |
Le-Bihanic F, Clérandeau C, Cormier B, et al. Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development [J]. Marine Pollution Bulletin, 2020(154): 111059.
|
| [55] |
Barboza L G, Vieira L R, Guilhermino L. Single and combined effects of microplastics and mercury on juveniles of the European seabass (Dicentrarchus labrax): changes in behavioural responses and reduction of swimming velocity and resistance time [J]. Environmental Pollution, 2018(236): 1014-1019.
|
| [56] |
Liu S L, Yan L, Zhang Y L, et al. Polystyrene nanoplastics exacerbated the ecotoxicological and potential carcinogenic effects of tetracycline in juvenile grass carp (Ctenopharyngodon idella) [J]. Science of the Total Environment, 2022(803): 150027.
|
| [57] |
Fonte E, Ferreira P, Guilhermino L. Temperature rise and microplastics interact with the toxicity of the antibiotic cefalexin to juveniles of the common goby (Pomatoschistus microps): post-exposure predatory behaviour, acetylcholinesterase activity and lipid peroxidation [J]. Aquatic Toxicology, 2016(180): 173-185.
|
| [58] |
Guo X Y, Cai Y P, Ma C X, et al. Combined toxicity of micro/nano scale polystyrene plastics and ciprofloxacin to Corbicula fluminea in freshwater sediments [J]. Science of the Total Environment, 2021(789): 147887.
|
| [59] |
殷岑. 微塑料和有机污染物对水生生物的联合毒性效应研究 [D]. 南京: 南京理工大学, 2018: 6-8.
Yin C. The combined toxic effect of microplastic and organic contaminants on aquatic organisms [D]. Nanjing: Nanjing University of Science and Technology, 2018: 6-8.
|
| [60] |
张哿, 邹亚丹, 徐擎擎, 等. 微塑料与水中污染物的联合作用研究进展 [J]. 海洋湖沼通报, 2019(2): 59-69. doi: 10.13984/j.cnki.cn37-1141.2019.02.009
Zhang G, Zou Y D, Xu Q Q, et al. Proceedings of joint effect of microplastics and pollutants in water [J]. Transactions of Oceanology and Limnology, 2019(2): 59-69. doi: 10.13984/j.cnki.cn37-1141.2019.02.009
|
DownLoad: