LEI La-mei, SONG Li-rong, LIU Yong-ding. COMPARISON OF GROWTH AND TOXIN ANALYSIS IN TWO PHENOTYPE MICROCYSTIS AERUGINOSA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 205-209.
Citation: LEI La-mei, SONG Li-rong, LIU Yong-ding. COMPARISON OF GROWTH AND TOXIN ANALYSIS IN TWO PHENOTYPE MICROCYSTIS AERUGINOSA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 205-209.

COMPARISON OF GROWTH AND TOXIN ANALYSIS IN TWO PHENOTYPE MICROCYSTIS AERUGINOSA

  • Received Date: July 18, 2000
  • Rev Recd Date: August 21, 2000
  • Published Date: May 24, 2001
  • Colonial Microcystis aeruginosa isolated from water-bloom of Dianchi Lake lost its colonial type and became single-celled type upon continuous subcultured in inorganic medium. It was observed that the growth rate of colonial M.aeruginosa was markedly lower than that of single cell; the extracellular acid polysaccharide capsule was obviously visible in the colonial type but was absent in the single-celled one; toxin analysis indicated that three microcystins isomers were produced in colonial M. aeruginosa, but in single-celled M. aeruginosa, only one major toxin-MC-LR was detected; sing celled M. aeruginosa had the LD 50 value almost ten times higher than colonial M. aeruginosa. Allozyme divergence was detected, too. The study has brought forward a new proof to explanation of mechanisms on regulation of microcystin synthesis.
  • [1]
    Botes D P,Tuiman A A,Wessels P L.The Structure of cyanoginosins-LA,a cyclic peptide from the cyanobacterium Microcystis aeruginosa [J].J.Chem.Soc.F Perkin Trans,1984,1:2311—2318[2] Lange W.Speculations on a possible essential function of the gelatinous sheath of blue-green algae [J].Can.J.Microbiol,1976,22:1181—1185[3] Shirai M,Matumaru K,Ohotake A,et.al.Development of a solid medium for growth and isolation of axenic Microcystis strains(Cyanobacteria)and improved procedure for purification of culture [J].Appl.Environ.Microbio1,1989,55:2569—2571[4] Bolch JSC,Blackburn IS.Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kütz.[J].J.Appl.Phycol.,1996,8:5—13[5] 宋立荣,雷腊梅,何振荣,等.滇池水华蓝藻铜锈微囊藻和绿色微囊藻的生长生理特性及毒素分析[J].水生生物学报,1999,23;402—407[6] 李敦海,宋立荣,刘永定.葛仙米光合活性对盐胁迫的反应[J].水生生物学报,1999,23;420—424[7] J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯著.分子克隆实验指南(第二版)[M].北京;科学出版社,1996[8] 胡能书,万贤国.同工酶技术及原理[M].长沙;湖南科学技术出版社,1985[9] Rippka R.Deruelles J,Waterbury J B,et al.Generic assignments,strain histories and properties of pure culture of cyanobacteria [J].J.Gen.Microbiol.,1979,111:1—61[10] Holm-Hansen O.Recent advance in the physiology of blue-green algae.Proceeding symposium on environmental requirement of blue-green algae [C].Fed.Water Pollut.Control Adm.,Pacific Northwest Water Lab.,Corvallis,Oregon.1967,87—96[11] Parker DL.Improved procedures for the cloning and purification of Microcystis cultures(Cyanophyta)[J].J.Phycol.,1982,18:471—477[12] Costerton J W.Structure and plasticity at various organization levels in the bacterial cell [J].Can.J.Microbiol.,1988,34:513—521[13] Plude J L,Parker D L,Schommer O J,et al.Chemical characterization of polysaccharide from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40[J].Appl.Environ.Microbiol.,1991,57:1696—1700[14] Martin C,Codd G A,Siegelman H W,et al.Lipopolysaccharides and polysaccharides of the cell envelope of toxic Microcystis aeruginosa strains [J].Arch.Microbiol.,1989,152:90—94[15] Forni C,Telo F R,Caiola M G.Comparative analysis of the polysaccharides produces by different species of Microcystis(Chroococcales,Cyanophyts)[J].Phycologia,1997,36:181—185[16] Nakagawa M.,Takamera Y.,Yagi O.Isolation and characterization of the slime from a cyanobacterium Microcystis aeruginosa K-3A [J] Agric.Biol.Chem.,1987,51:329—337[17] Shi L,Carmichael W W,Miller I.Immuno-gold localization of hepatotoxins in cyanobacterial cells [J].Arch.Microbiol.,1995,163:7—15

    Botes D P,Tuiman A A,Wessels P L.The Structure of cyanoginosins-LA,a cyclic peptide from the cyanobacterium Microcystis aeruginosa [J].J.Chem.Soc.F Perkin Trans,1984,1:2311—2318[2] Lange W.Speculations on a possible essential function of the gelatinous sheath of blue-green algae [J].Can.J.Microbiol,1976,22:1181—1185[3] Shirai M,Matumaru K,Ohotake A,et.al.Development of a solid medium for growth and isolation of axenic Microcystis strains(Cyanobacteria)and improved procedure for purification of culture [J].Appl.Environ.Microbio1,1989,55:2569—2571[4] Bolch JSC,Blackburn IS.Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kütz.[J].J.Appl.Phycol.,1996,8:5—13[5] 宋立荣,雷腊梅,何振荣,等.滇池水华蓝藻铜锈微囊藻和绿色微囊藻的生长生理特性及毒素分析[J].水生生物学报,1999,23;402—407[6] 李敦海,宋立荣,刘永定.葛仙米光合活性对盐胁迫的反应[J].水生生物学报,1999,23;420—424[7] J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯著.分子克隆实验指南(第二版)[M].北京;科学出版社,1996[8] 胡能书,万贤国.同工酶技术及原理[M].长沙;湖南科学技术出版社,1985[9] Rippka R.Deruelles J,Waterbury J B,et al.Generic assignments,strain histories and properties of pure culture of cyanobacteria [J].J.Gen.Microbiol.,1979,111:1—61[10] Holm-Hansen O.Recent advance in the physiology of blue-green algae.Proceeding symposium on environmental requirement of blue-green algae [C].Fed.Water Pollut.Control Adm.,Pacific Northwest Water Lab.,Corvallis,Oregon.1967,87—96[11] Parker DL.Improved procedures for the cloning and purification of Microcystis cultures(Cyanophyta)[J].J.Phycol.,1982,18:471—477[12] Costerton J W.Structure and plasticity at various organization levels in the bacterial cell [J].Can.J.Microbiol.,1988,34:513—521[13] Plude J L,Parker D L,Schommer O J,et al.Chemical characterization of polysaccharide from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40[J].Appl.Environ.Microbiol.,1991,57:1696—1700[14] Martin C,Codd G A,Siegelman H W,et al.Lipopolysaccharides and polysaccharides of the cell envelope of toxic Microcystis aeruginosa strains [J].Arch.Microbiol.,1989,152:90—94[15] Forni C,Telo F R,Caiola M G.Comparative analysis of the polysaccharides produces by different species of Microcystis(Chroococcales,Cyanophyts)[J].Phycologia,1997,36:181—185[16] Nakagawa M.,Takamera Y.,Yagi O.Isolation and characterization of the slime from a cyanobacterium Microcystis aeruginosa K-3A [J] Agric.Biol.Chem.,1987,51:329—337[17] Shi L,Carmichael W W,Miller I.Immuno-gold localization of hepatotoxins in cyanobacterial cells [J].Arch.Microbiol.,1995,163:7—15
  • Related Articles

    [1]GUO Xiao-Ye, LI Yan-Hua, HAN Dan-Xiang. ANALYSIS OF LIPIDOMES OF THE SYNECHOCYSTIS SP. PCC 6803 CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(2): 376-386. DOI: 10.7541/2021.2019.254
    [2]LI Xiao-Dong, WANG Ya-Li, GAO Hong, XU Xu-Dong, KONG Ren-Qiu. APPLICATION OF THE GVPA-C INTERGENIC SEQUENCE IN TYPING OF MICROCYSTIS[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(5): 1098-1103. DOI: 10.7541/2019.129
    [3]SHI Jun-Qiong, YANG Yan-Jun, DONG Cong-Cong, ZHANG Hong-Bo, WU Zhong-Xing. EFFECTS OF OCHROMONAS SP. CULTURE MEDIA FILTRATE ON THE GROWTH AND PHYSIOLOGICAL TRAITS OF MICROCYSTIS AERUGINOSA[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(1): 213-218. DOI: 10.7541/2019.026
    [4]LIANG Ye, OUYANG Kai, XU Huan-Huan, YANG Jia-Xin. EFFECTS OF MICROCYSTIS AERUGINOSA ON THE LIFE-TABLE PARAMETERS AND PHENOTYPIC TRAITS OF ROTIFERS[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(6): 1362-1368. DOI: 10.7541/2017.168
    [5]LI Na, BI Yong-Hong, GAO Da-Wen, HU Zheng-Yu, REN Nan-Qi. EFFECTS OF ELEVATED CO2 CONCENTRATION ON GROWTH OF MICROCYSTIS AERUGINOSA[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(4): 698-702. DOI: 10.3724/SP.J.1035.2011.00698
    [6]FU Wen-Yu, WANG Mi-Mi, XU Li-Hong. EFFECT OF MICROCYSTIN-LR ON CASPASE-3 IN RAT HEPATOCYTES[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(4): 452-453.
    [7]Chen Yueqin, He Jiawan, Zhuang Li, Zeng Longmei, Qu Lianghu. SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(1): 41-46.
    [8]Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.
    [9]Li Shaorong, Lin Huimin. PURIFICATION AND CHARACTERIZATION OF PHYCOERYTHRIN FROM RHODOSORUS MARINUS[J]. ACTA HYDROBIOLOGICA SINICA, 1996, 20(3): 257-264.
    [10]Xu Ping, Tseng Chaotsi. UPTAKE OF AMINO ACIDS BY TOXIC MICROCYSTIS AERUGINOSA PCC 7820[J]. ACTA HYDROBIOLOGICA SINICA, 1994, 18(2): 180-182.

Catalog

    Article views (908) PDF downloads (426) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return