YU Guo-Ce, CONG Wei, CAI Zhao-Ling, SHI Ding-Ji, OUYANG Fan. EFFECTS OF ORGANIC COMPOUNDS ON THE GROWTH OF ANABAENA sp. 7120[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(3): 238-242.
Citation: YU Guo-Ce, CONG Wei, CAI Zhao-Ling, SHI Ding-Ji, OUYANG Fan. EFFECTS OF ORGANIC COMPOUNDS ON THE GROWTH OF ANABAENA sp. 7120[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(3): 238-242.

EFFECTS OF ORGANIC COMPOUNDS ON THE GROWTH OF ANABAENA sp. 7120

  • Received Date: November 14, 2001
  • Rev Recd Date: October 29, 2002
  • Published Date: May 24, 2003
  • Cultures of Anabaena sp. 7120 were carried out in nitrogen free BG 11 medium in an orbital shaker at 130 r/min and 30℃ at a light intensity of 160 μE m-2 s-1 unless otherwise specified. Various concentrations of organic carbon compounds were contained in the medium to examine their effects on the growth of Anabaena sp. 7120 cells as indicated by the cell density and the chlorophyll concentration. Glucose was shown to be able to improve cell growth markedly. With the glucose concentration varying within a range from 0 to 30 g/L, the maximal cell density in mixotrophic growth was able to reach 3.4 folds of that in photoautotrophic growth. Glucose was utilized to a limited extent in mixotrophic cultures. Cell growth at various light intensities indicated that the saturation light intensity for mixotrophic growth with a glucose concentration of 3 g/L was around 80 μE m-2 s-1. Acetate was found to impede cell growth to some extent when examined within a concentration range from 0 to 1.5 g/L. Sucrose, ethanol and glycerol exerted slight influence on cell growth, whereas lactate, citrate, glutamate and glycine inhibited cell growth seriously. Glucose, acetate, sucrose, glycerol and ethanol did not cause significant changes in photosynthetic oxygen evolution and dark respiratory oxygen consumption while lactate, citrate and glutamate destroyed these activities. Anabaena sp. 7120 did not exhibit heterotrophic growth on glucose in complete darkness or light activated heterotrophic growth on glucose with a daily pulse of light of 5 min of 160 μE m-2 s-1. However, comparison of cell growth in the presence of 10-5 M DCMU with and without glucose in light suggested that slight photoheterotrophy may exist.
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    Droop M R. Heterotrophy of carbon [M]. California: University of California Press, 1974,530-559[2] Smith A J. Modes of cyanobacterial carbon metabolism [M]. London: Blackwell Scientific Publications. 1982,47-85[3] Tuchman N C. The role of heterotrophy in algae [M]. San Diego:Academic Press. 1996, 299-319[4] Chen F. High cell density culture of microalgae in heterotrophic growth [J].Tibtech, 1996,14:421-426[5] Ren L, Shi D J, Dai J X, et al. Expression of the mouse metallothionein-I gene conferring cadmium resistance in a transgenic cyanobacterium [J].FEMS Microbiol. Letts., 1998,158:127-132[6] Liu F L, Zhang H B, Shi D J, et al. Construction of shuttle, expression vector of human tumor necrosis factor alpha(hTNF-α)gene and its expression in a cyanobacterium, Anabaena sp. 7120 [J].Science in China(Series C), 1999,42:25-33[7] Williams J G K. Construction of specific mutations in photosystem Ⅱ photosynthetic reaction center by genetic engineering methods in Synechocystis 6803 [M]. San Diego:Academic Press, 1988,766-778[8] Zhang L X, Zhang T F LI L Y. Methods and Techniques in Biochemical Experiments [M]. Beijing: Higher Education Press.1981,6-9.[张龙翔,张庭芳,李令媛.生化实验方法和技术.北京:高等教育出版社,1981,6-9][9] Cheung W Y, Gibbs M. Dark and photometabolism of sugars by a blue green alga: Tolypothrix tenuis [J].Plant Physiol., 1966,41:731-737[10] Pearce J, Carr N G. The incorporation and metabolism of glucose by Anabaena variabilis [J].J. Gen. Microbiol.,1969,54:451-462[11] Pelroy R A, Rippka R, Stanier R Y. Metabolism of glucose by unicellular blue-green algae [J].Arch. Mikrobiol., 1972,87:303-322[12] Pearce J, Leach C K, Carr N G. The incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis [J].J. Gen. Microbiol.,1969,55:371-378[13]Martinez F, Orus M I. Interactions between glucose and inorganic carbon metabolism in Chlorella vulgaris strain UAM 101 [J].Plant Physiol.,1991,95:1150-1155[14] Drews G. Fine structure and chemical compositon of the cell envelopes [M]. London:Blackwell Scientific Publication.1973,99-116[15] Drews G, Weckesser J. Function, structure and composition of cell walls and external layers [M]. London:Blackwell Scientific Publications.1982,333-357[16] Kiyohara T, Fujima Y, Hattori A et al. Effect of light on glucose assimilation in Tolypothrix tenuis [J].J. Gen. Appl. Microbiol.,1962,8:165-168[17] Hoare D S, Hoare S L, Mcore R B. The photo-assimilation of organic compounds by autotrophic blue-green algae [J].J. Gen. Microbiol.,1967,49:351-370[18] Van Baalen C, Hoare D S, Brandt E. Heterotrophic growth of blue-green algae in dim light[J].J. Bact.,1971,105:685-689[19] Fay P. Heterotrophy and nitrogen fixation in Chlorogloea fritschii Mitra [J]. J. Gen. Microbiol.,1965,39:11-20[20] JIN C Y Physiology of heterotrophic growth of blue-green alga Anabaena azollae [J].Acta Hydrobiologica Sinica,1984,8(4):443-448.[金传荫.满江红鱼腥藻的异养生长.水生生物学集刊,1984,8(4):443-448][21] JIN C Y, SONG L R,LI S H. The mixotrophic growth of Anabaena sp. HB1017[J].Acta Hydrobiologica Sinica,1996,20(2):134-137.[金传荫,宋立荣,黎尚豪.鱼腥藻1017株的混合营养型生长.水生生物学报 1996,20(2):134-137][22] Rippka R. Photoheterotrophy and chemoheterotrophy among unicellular blue-green algae [J].Arch. Mikrobiol.,1972,87:93-98[23] Anderson A L, McIntosh L. Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803:a blue-light-requiring process [J].J.Bacteriol.,1991,173:2761-2767
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