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ZHAO Jun, CUI Miao, CHEN Xiang-Lin. ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 33-39.
Citation: ZHAO Jun, CUI Miao, CHEN Xiang-Lin. ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 33-39.
shu

ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)

  • Life science college of South China Normal University, Guangzhou510631

  • Received Date: October 11, 2002
  • Rev Recd Date: June 05, 2003
  • Published Date: January 24, 2004
    Graphical Abstract
    • Abstract
  • Pengze crucian carp, just like silver crucia n carp, is known as gynoge netic crucian carp. It is one of the most popular cultural fishes in China. The offspring of pengze crucian carp activated by heterogeneous sperms show obvious allogynogenetic biological effects in growth, rate of fertilization and other as pects. But like in silver crucian carp, no evidences have been found that the al logynogenetic biological effects exist in morphology or protein phenotype in pen gze crucian carp. That is why there are argument about the phenomenon of allogyn ogenetic biological effects. In this paper, the patterns of expressions in four isozymes (EST, MDH, ME and LDH) were investigated in the embryogenetic stages of gynogenetic crucian carp (Carassius auratus of Pengze) activated by homogen ous and heterogeneous sperms, respectively, used by polyacrylamide gel electroph oresis. The material used here were collected from Nanhai Aquiculture Experiment Base, College of Life Science, South China Normal University. Both MDH and LDH showed apparent differences between the embryos activated by homogenous sperm an d those by heterogeneous sperm. The main differences in MDH exist at hatching st age, which showed two more bands in the isozyme pattern of embryos activated by heterogeneous sperm than those of embryos activated by homogenous sperm. The mai n differences in LDH exist at five stages from middle-gastrula to muscle constr uction, which showed four more bands in the isozyme pattern of embryos activated by heterogeneous sperm than those of embryos activated by homogenous sperm. Mea nwhile, the other two isozymes (EST and ME) detected in this paper did not show apparent differences between the embryos activated by homogenous sperm and those activated by heterogeneous sperm. Furthermore, the speed of development of the embryos activated by heterogeneous sperm is slight faster than that of the embry os activated by homogenous sperm. Although it is difficult to define the reason of the above differences, it can be considered as one form of allogynogenetic bi ological effects.
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  • [1]
    Jiang Y G, Liang S C, Chen B D, et al. Biological effect of heterlogous sperm on gynogenetic offspring in Carassius auratus gibelio [J]. Acta Hydrobiologica sinaca, 1983, 8(1):1-13.[蒋一NB028,梁绍昌,陈本德. 异源精子在银鲫雌核发育子代中的生物学效应. 水生生物学报,1983,8(1):1-13][2] Ding J, Xie Y F, Jiang Y G, et al. The analysis of heterologous genetic materials in allogenetic crucian carp and its artificial hybrids [J]. Acta Hydrobiologica sinaca, 1993, 17(1):22-26.[丁军,谢岳峰,蒋一NB028等. 异育银鲫及其人工杂合种外源遗传物质的检测分析. 水生生物学报,1993,17(1):22-26][3] Ding J, Jiang Y G, Shan S X, et al. Cytological mechanism on the appearance of multiple tetraploid allogynogenetic crucian carp [J]. Acta Hydrobiologica sinaca, 1992, 16(2):186-187.[丁军,蒋一NB028,单士新, 等. 复合四倍体银鲫产生的细胞学机制. 水生生物学报,1992,16(2):186-187][4] Chen H, Yang J, Xue G X, et al. The application of RAPD in allogynogenetic silver crucian carp (Carassius auratus gibelio)[J]. Chinese Science Bulletin, 1994,39(7):661-664].[陈洪,杨靖,薛国雄, 等. RAPD在异精激发方正银鲫比较研究中的应用. 科学通报,1994,39(7):661-663][5] Zhang H F, Chen X L, Shu H, et al. RAPD analysis of gynogenetic F1 progeny triggering by heterologous sperms and their parents of Pengze crucian carp(Carassius auratus)[J]. Chin J Appl Environ Biol, 1999, 5(5):507-511.[张海发,陈湘NB024,舒琥, 等. 异源精子激发彭泽鲫雌核发育产生的子一代及亲本RAPD分析. 应用与环境生物学报,1999,5(5):507-511][6] Chen X L, Zhao J, Ma G Z, et al. Application of gynogenetic crucian carp in fish breeding [J]. Journal of South China Normal University(Natural Science), 1998, supplement: 1-3.[陈湘NB024,赵俊,马广智,等. 雌核发育鲫鱼在育种上的应用. 华南师范大学学报(自然科学版),1998,(增刊):1-3][7] Schartl M, Nandal I and Schlupp I et al., Incorporation of subgenomic amounts of host species DNA in the gynogenetic Amazon molly [J]. Nature, 1995, 373: 67-71[8] Lu Y D, Shen H, Lu J and Shi H. Comparative studies on biochemical composition in serum of allogynogenetic Qihe crucian carp and its parents [J]. Zool Res, 1991, 12(2):181-185.[楼允东,沈NB058,陆君,时宏. 异育淇鲫及其亲本血清生化组成的比较研究. 动物学研究,1991,12(2):181-185][9] Zhang Y P, Liu H, Lou Y D. Electrophoresis of isozymes and muscle protein of an abnormal crucian carp[J]. Acta Genetica Sinica, 1990, 17(1):34-37.[张英培,刘红,楼允东. 异育淇鲫及其双亲同工酶的比较研究. 遗传学报,1990,17(1):34-37][10] Teng C B, Sun X W and Shen J B. RAPD analysis of gynogenetic crucian carp activiated by heterologous sperms and their parents [J]. Journal of Fisheries of China, 1999, 23(4): 420-423.[滕春波,孙孝文,沈俊宝. 利用异源精子激发雌核发育的银鲫及亲本的RAPD分析. 水产学报,1999,23(4):420-423][11] Zhou L, Wang Y, Gui J F. Genetic evidence for gonochoritic reproduction in gynogenetic silver crucian carp (Carassius auratus gobelio Block) as revealed by RAPD assays [J]. J Mol Evol, 2000a,51:498-506[12] Zhu L F. Gradient Gel Electrophoresis on polyacrylamide of isozymes and proteins of fishes [J]. Acta Hydrobiologica sinaca, 1992,16(2): 183-185.[朱蓝菲. 鱼类同工酶和蛋白质的聚丙烯酰胺凝胶电泳法. 水生生物学报,1992,16(2):183-185][13] Zhou L and Gui J F. Karyotypic dicersity in polyploid gibel carp, Carassius auratus gobelio Block [J]. Genetica, 2002, 115:223-232[14] Zhou L, Wang Y, Gui J F. Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers [J]. Cytogenet Cell Genet, 2000b,88:133-139[15] Li M Y, Zhou L, Yang L, et al. Molecular genetic analysis on Pengze crucian carp and comparison with strain A of gynogenetic silver crucian carp [J]. Journal of Fisheries China, 2002, 26(5): 472-476.[李名友,周莉,杨林,等. 彭泽鲫的分子遗传分析及其与方正银鲫A系的比较. 水产学报,2002,26(5):472-476][16] Yao J H, Lou Y D, Jiang Y. A study on mitochondrial DNA polymorphis among six geographical populations of Carassius auratus gobelio in China [J]. Journal of Fisheries of China, 1998, 22(4): 289-295. [姚纪花,楼允东,江涌. 我国六个地区银鲫种群线粒体DNA多态性的研究. 水产学报,1998,22(4):289-295][17] Zhang H, Dong X H, Ye Y Z and Wu Q J. Comparative studies of the mtDNA from three strains of triploid Carassius auratus and Carassius auratus auratus [J]. Acta Genetica Sinica, 1998, 25(4): 330-336. [张辉,董新红,叶玉珍, 等. 三个三倍体鲫鱼品系及野鲫mtDNA的比较研究. 遗传学报,1998,25(4):330-336][18] Yang R J, Li B X, Feng H, Yang C B, Sun Y D, Zhang X J and Luo S. 2003. Cytogenetic analysis of chromosome number and ploidy of Carassius auratus variety pengze [J]. Acta Zoologica Sinica, 49(1): 104-109. [杨睿娇,李冰霞,冯浩,杨楚彬,孙远东,张轩杰,罗NB056. 2003. 彭泽鲫染色体数目及倍性的细胞遗传学分析. 动物学报,49(1):104-109][19] Wu L Z and Wang Z X. Study on the developmental genetic of isozymes in Grass carp (Ctenopharyngodon idellus) Ⅱ. Analysis of isozymes during the early development of Grass carp [J]. Acta Genetica Sinica, 1987, 14(5): 387-394.[吴力钊,王祖熊. 草鱼同工酶发育遗传学研究. 遗传学报,1987,14(5):387-394]

    Jiang Y G, Liang S C, Chen B D, et al. Biological effect of heterlogous sperm on gynogenetic offspring in Carassius auratus gibelio [J]. Acta Hydrobiologica sinaca, 1983, 8(1):1-13.[蒋一NB028,梁绍昌,陈本德. 异源精子在银鲫雌核发育子代中的生物学效应. 水生生物学报,1983,8(1):1-13][2] Ding J, Xie Y F, Jiang Y G, et al. The analysis of heterologous genetic materials in allogenetic crucian carp and its artificial hybrids [J]. Acta Hydrobiologica sinaca, 1993, 17(1):22-26.[丁军,谢岳峰,蒋一NB028等. 异育银鲫及其人工杂合种外源遗传物质的检测分析. 水生生物学报,1993,17(1):22-26][3] Ding J, Jiang Y G, Shan S X, et al. Cytological mechanism on the appearance of multiple tetraploid allogynogenetic crucian carp [J]. Acta Hydrobiologica sinaca, 1992, 16(2):186-187.[丁军,蒋一NB028,单士新, 等. 复合四倍体银鲫产生的细胞学机制. 水生生物学报,1992,16(2):186-187][4] Chen H, Yang J, Xue G X, et al. The application of RAPD in allogynogenetic silver crucian carp (Carassius auratus gibelio)[J]. Chinese Science Bulletin, 1994,39(7):661-664].[陈洪,杨靖,薛国雄, 等. RAPD在异精激发方正银鲫比较研究中的应用. 科学通报,1994,39(7):661-663][5] Zhang H F, Chen X L, Shu H, et al. RAPD analysis of gynogenetic F1 progeny triggering by heterologous sperms and their parents of Pengze crucian carp(Carassius auratus)[J]. Chin J Appl Environ Biol, 1999, 5(5):507-511.[张海发,陈湘NB024,舒琥, 等. 异源精子激发彭泽鲫雌核发育产生的子一代及亲本RAPD分析. 应用与环境生物学报,1999,5(5):507-511][6] Chen X L, Zhao J, Ma G Z, et al. Application of gynogenetic crucian carp in fish breeding [J]. Journal of South China Normal University(Natural Science), 1998, supplement: 1-3.[陈湘NB024,赵俊,马广智,等. 雌核发育鲫鱼在育种上的应用. 华南师范大学学报(自然科学版),1998,(增刊):1-3][7] Schartl M, Nandal I and Schlupp I et al., Incorporation of subgenomic amounts of host species DNA in the gynogenetic Amazon molly [J]. Nature, 1995, 373: 67-71[8] Lu Y D, Shen H, Lu J and Shi H. Comparative studies on biochemical composition in serum of allogynogenetic Qihe crucian carp and its parents [J]. Zool Res, 1991, 12(2):181-185.[楼允东,沈NB058,陆君,时宏. 异育淇鲫及其亲本血清生化组成的比较研究. 动物学研究,1991,12(2):181-185][9] Zhang Y P, Liu H, Lou Y D. Electrophoresis of isozymes and muscle protein of an abnormal crucian carp[J]. Acta Genetica Sinica, 1990, 17(1):34-37.[张英培,刘红,楼允东. 异育淇鲫及其双亲同工酶的比较研究. 遗传学报,1990,17(1):34-37][10] Teng C B, Sun X W and Shen J B. RAPD analysis of gynogenetic crucian carp activiated by heterologous sperms and their parents [J]. Journal of Fisheries of China, 1999, 23(4): 420-423.[滕春波,孙孝文,沈俊宝. 利用异源精子激发雌核发育的银鲫及亲本的RAPD分析. 水产学报,1999,23(4):420-423][11] Zhou L, Wang Y, Gui J F. Genetic evidence for gonochoritic reproduction in gynogenetic silver crucian carp (Carassius auratus gobelio Block) as revealed by RAPD assays [J]. J Mol Evol, 2000a,51:498-506[12] Zhu L F. Gradient Gel Electrophoresis on polyacrylamide of isozymes and proteins of fishes [J]. Acta Hydrobiologica sinaca, 1992,16(2): 183-185.[朱蓝菲. 鱼类同工酶和蛋白质的聚丙烯酰胺凝胶电泳法. 水生生物学报,1992,16(2):183-185][13] Zhou L and Gui J F. Karyotypic dicersity in polyploid gibel carp, Carassius auratus gobelio Block [J]. Genetica, 2002, 115:223-232[14] Zhou L, Wang Y, Gui J F. Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers [J]. Cytogenet Cell Genet, 2000b,88:133-139[15] Li M Y, Zhou L, Yang L, et al. Molecular genetic analysis on Pengze crucian carp and comparison with strain A of gynogenetic silver crucian carp [J]. Journal of Fisheries China, 2002, 26(5): 472-476.[李名友,周莉,杨林,等. 彭泽鲫的分子遗传分析及其与方正银鲫A系的比较. 水产学报,2002,26(5):472-476][16] Yao J H, Lou Y D, Jiang Y. A study on mitochondrial DNA polymorphis among six geographical populations of Carassius auratus gobelio in China [J]. Journal of Fisheries of China, 1998, 22(4): 289-295. [姚纪花,楼允东,江涌. 我国六个地区银鲫种群线粒体DNA多态性的研究. 水产学报,1998,22(4):289-295][17] Zhang H, Dong X H, Ye Y Z and Wu Q J. Comparative studies of the mtDNA from three strains of triploid Carassius auratus and Carassius auratus auratus [J]. Acta Genetica Sinica, 1998, 25(4): 330-336. [张辉,董新红,叶玉珍, 等. 三个三倍体鲫鱼品系及野鲫mtDNA的比较研究. 遗传学报,1998,25(4):330-336][18] Yang R J, Li B X, Feng H, Yang C B, Sun Y D, Zhang X J and Luo S. 2003. Cytogenetic analysis of chromosome number and ploidy of Carassius auratus variety pengze [J]. Acta Zoologica Sinica, 49(1): 104-109. [杨睿娇,李冰霞,冯浩,杨楚彬,孙远东,张轩杰,罗NB056. 2003. 彭泽鲫染色体数目及倍性的细胞遗传学分析. 动物学报,49(1):104-109][19] Wu L Z and Wang Z X. Study on the developmental genetic of isozymes in Grass carp (Ctenopharyngodon idellus) Ⅱ. Analysis of isozymes during the early development of Grass carp [J]. Acta Genetica Sinica, 1987, 14(5): 387-394.[吴力钊,王祖熊. 草鱼同工酶发育遗传学研究. 遗传学报,1987,14(5):387-394]
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