PROKARYOTIC EXPRESSION OF VP60B OF SHRIMP WHITE SPOT SYNDROME VIRUS AND PRIMARY ANALYSIS ON ITS AMINO ACID SEQUENCE

White spot syndrome virus (WSSV) from shrimp was first found in Taiwan in 1992. Since 1993, white spot syndrome disease of shrimp caused by this virus was widely spread throughout China,Asia and Pan?Pacific Ocean. It can cause 100% cumulative mortality of cultured shrimps in 3-4 days. The virus particles contain fivemajor proteins: VP28, VP26, VP24, VP19 and VP15.The mode of entry and systemic infection of WSSV in shrimp and the funotions of these virion proteins are not clear. VP60B is a kind of minor structure proteins in WSSV particles. Using interproscan to analysis on homology to VP60B, a piece of sequence of amino acid in VP60Bwas found homology with adenovirus type 5 fiber protein knob domain. In this study, thegene of VP60B had been cloned into a prokaryotic expression system, and induced to express at low temperature. The result showed that the expressed protein was existed mainly as inclusion body in this expression system. Prokaryotic expressed VP60B could not recognize by polyclone antiserum against WSSV, which suggested that this protein might be aweak immunogen. According to the analysis of the sequence of amino acid, a trans?membrane domain was found, which suggested that this protein might locate on the envelop membrane of WSSV.