CuSO4和ZnSO4对尼罗罗非鱼N-乙酰--D-氨基葡萄糖苷酶的影响
EFFECTS OF CuSO4 AND ZnSO4 ON N-ACETYL--D-GLUCOSAMINIDASE FROM OREOCHROMIS NILOTICUS
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摘要: 以分离自尼罗罗非鱼(Oreochromis niloticus)精巢的N-乙酰--D-氨基葡萄糖苷酶(EC 3.2.1.52, NAGase)为研究对象,探讨了两种水产常用药物CuSO4和ZnSO4对NAGase的影响。研究结果表明CuSO4和ZnSO4对该酶抑制的IC50分别为(1.230.05)和(0.280.02) mmol/L,都能改变酶的构象从而影响到其内源荧光的发射。这两种药物对该酶的抑制机理均为可逆抑制,其中CuSO4对酶的抑制类型为非竞争型, ZnSO4为竞争型,且均能明显影响该酶的pH稳定性和热稳定性。研究结果为罗非鱼养殖过程中CuSO4和ZnSO4的使用和监控提供了参考。
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关键词:
- 尼罗罗非鱼 /
- N-乙酰--D-氨基葡萄糖苷酶 /
- CuSO4 /
- ZnSO4
Abstract: N-Acetyl--D-glucosaminidase(EC 3.2.1.52, NAGase), hydrolyzing the oligomers of N-Acetyl--D-glucosamine(NAG) into monomer, is correlated with animal reproduction. In the current study, we investigated the effect of two common drugs(CuSO4 and ZnSO4) on NAGase from spermary of Nile tilapia(Oreochromis niloticus). The results showed that the IC50 of CuSO4 and ZnSO4 were(1.230.05) mmol/L and(0.280.02) mmol/L, respectively. Both CuSO4 and ZnSO4 regulated the conformation of tilapia NAGase, which affected the fluorescence emission of the enzyme. The inhibitory activities of these two drugs were reversible, and the inhibition type of CuSO4 was noncompetitive but ZnSO4 was competitive. Both CuSO4 and ZnSO4 had significant influences on the thermal and pH stability of the enzyme. The results contribute to the use and control of CuSO4 and ZnSO4 in tilapia culture.-
Keywords:
- Oreochromis niloticus /
- N-acetyl--D-glucosaminidase /
- CuSO4 /
- ZnSO4
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