爪哇曲壳藻亚缢变种胞外多聚物提取方法及其化学组成研究

黄世玉

黄世玉. 爪哇曲壳藻亚缢变种胞外多聚物提取方法及其化学组成研究[J]. 水生生物学报, 2016, 40(3): 589-593. DOI: 10.7541/2016.79
引用本文: 黄世玉. 爪哇曲壳藻亚缢变种胞外多聚物提取方法及其化学组成研究[J]. 水生生物学报, 2016, 40(3): 589-593. DOI: 10.7541/2016.79
HUANG Shi-Yu. EXTRACTION METHOD AND CHEMICAL COMPOSITION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM DIATOM ACHNANTHES JAVANICA VAR. SUBCONSTRICTA[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(3): 589-593. DOI: 10.7541/2016.79
Citation: HUANG Shi-Yu. EXTRACTION METHOD AND CHEMICAL COMPOSITION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM DIATOM ACHNANTHES JAVANICA VAR. SUBCONSTRICTA[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(3): 589-593. DOI: 10.7541/2016.79

爪哇曲壳藻亚缢变种胞外多聚物提取方法及其化学组成研究

基金项目: 

国家自然科学基金(39900020)资助

EXTRACTION METHOD AND CHEMICAL COMPOSITION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM DIATOM ACHNANTHES JAVANICA VAR. SUBCONSTRICTA

Funds: 

Supported by the National Natural Science Foundation of China (39900020)

  • 摘要: 优化了爪哇曲壳藻亚缢变种(Achnanthes javanica var. subconstricta)胞外多聚物提取方法并分析了其化学组成。结果表明, 使用0.3 mol/L的碳酸氢钠提取时间为2.53h可充分提取非水溶性胞外多聚物而不会破碎细胞, 是最优的提取方法。爪哇曲壳藻亚缢变种胞外多聚物多糖占80%以上, 蛋白质占16%19%, 不含脂类。水溶性多糖的单糖组分摩尔比为: 岩藻糖:半乳糖:葡萄糖:甘露糖:木糖:鼠李糖=2.47:2.13:1:0.69:0.57:0.46,而非水溶性多糖的单糖组分摩尔比为: 半乳糖:岩藻糖:木糖:葡萄糖:甘露糖:鼠李糖=3.56:2.73:1.30:1:0.67:0.57。
    Abstract: The current study reported the method to extract extracellular polymeric substances (EPSs) from Achnanthes javanica var. subconstricta and analyzed compositions of the EPSs. The results showed that the optimal extraction condition was 0.3 mol/L NaHCO3 with 2.53h extraction time, which produced little lysis. Polysaccharide is the main component of EPSs that contributed more than 80% of EPSs, while protein contributed 16%19% of EPSs. There is no lipid in the EPSs. The monosaccharide composition of flucose, galactose, glucose, mannose, xylose, and rhamnose in water-soluble polysaccharide is 2.47:2.13:1:0.69:0.57:0.46 (molar ratio). The monosaccharide composition of galactose, flucose, xylose, glucose, mannose, and rhamnose in non-water-soluble polysaccharide is 3.56:2.73:1.30:1:0.67:0.57.
  • [1]

    Steele D J, Franklin D J, Underwood G J C. Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms [J]. Biofouling, 2014, 30(8): 987998

    [2]

    Taylor J D, McKew B A, Kuhl A, et al. Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuel both generalist and specialist EPS-degrading bacteria [J]. Limnology and Oceanography, 2013, 58(4): 14631480

    [3]

    Lin H, Zhang M, Wang F, et al. A critical review of extracellular polymeric substances (EPSs) in membrane bioreactors: characteristics, roles in membrane fouling and control strategies [J]. Journal of Membrane Science, 2014, 460: 110125

    [4]

    Salama Y, Chennaoui M, Sylla A, et al. Characterization, structure, and function of extracellular polymeric substances (EPS) of microbial biofilm in biological wastewater treatment systems: a review [J]. Desalination Water Treatment, 2015: 118

    [5]

    Takahashi E, Ledauphin J, Goux D, et al. Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods [J]. Marine and Freshwater Research, 2009, 60: 12011210

    [6]

    Subramanian S B, Yan S, Tyagi R D, et al. Extracellular polymeric substances (EPS) producing bacterial strains of municipal wastewater sludge: isolation, molecular identification, EPS characterization and performance for sludge settling and dewatering.[J]. Water Research, 2010, 44(44): 22532266

    [7]

    Lin H, Zhang M, Wang F, et al. A critical review of extracellular polymeric substances (EPSs) in membrane bioreactors: Characteristics, roles in membrane fouling and control strategies [J]. Journal of Membrane Science, 2014, 460(9): 110125

    [8]

    Leiyan Z, Hongqiang R, Lili D. Comparison of extracellular polymeric substances (EPS) extraction from two different activated sludges [J]. Water Science Technology, 2012, 66(7): 15581564

    [9]

    Yang J L, Wang C, Gu Z Q, et al. A review on the role of marine biofilms on larval settlement and metamorphosis of marine invertebrates [J]. Marine Sciences, 2012, 36(8): 116121 [杨金龙, 王冲, 顾忠旗, 等. 微生物膜对海洋无脊椎动物幼体附着变态的影响研究. 海洋科学, 2012, 36(8): 116121]

    [10]

    Dubois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances [J]. Analytical Chemistry, 1956, 28: 350356

    [11]

    Lowry O H, RosebrotIgh N J, Farr A L, et al. Protein measurement with the Folin phenol reagent [J]. The Journal of Biological Chemistry, 1951, 193: 265275

    [12]

    Montani H, Crignis M G D, Lavaud J. Viral impact on prokaryotic and microalgal activities in the microphytobenthic biofilm of an intertidal mudflat (French Atlantic Coast) [J]. Frontiers in Microbiology, 2015, (6): 115

    [13]

    Wustman B A, Gretz M R, Hoagland K D. Extracellular matrix assembly in diatoms (Bacillariophyceae). I. A model of adhesives based on chemical characterization and localization of polysaccharides from the marine dia- tom Achnanthes longipes and other diatoms [J]. Plant Physiology, 1997, 113: 10591069

    [14]

    Dong D M, Kang C L, Li Z H, et al. Extarction of extracellular polymers from bioiflms in natural water by potassium Bi-phosphate solution [J]. Science Technology and Engineering, 2002, 2(5): 5153 [董得明, 康春莉, 李忠华, 等. 磷酸氢二钾法分离自然水体中的生物膜胞外聚合物. 科学技术与工程, 2002, 2(5): 5153]

    [15]

    Suresh V, Senthilkumar N, Thangam R, et al. Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity [J]. Process Biochemistry, 2013, 48(2): 364373

    [16]

    Urbani R, Pletikapi? G, Sist P, et al. Diatom Polysacchari- des: Extracellular Production, Isolation and Molecular Characterization [M]. INTECH Open Access Publisher. 2012, 345370

    [17]

    Xu L L, Zhan X R, Zeng Z W, et al. Study on preparation and chemical characterization of iron (III) - Polygonatum odoratum polysaccharides complex [J]. Journal of Chinese Pharmaceutical Sciences, 2012, 47(5): 331334

    [18]

    Nicolas V O, Moerdijk-Poortvliet T C W, Boschker H T S, et al. Release of dissolved carbohydrates by Emiliania huxleyi and formation of transparent exopolymer particles depend on algal life cycle and bacterial activity [J]. Envi- ronmental Microbiology, 2013, 15(5): 15141531

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出版历程
  • 收稿日期:  2015-10-18
  • 修回日期:  2016-02-16
  • 发布日期:  2016-05-24

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