爪哇曲壳藻亚缢变种胞外多聚物提取方法及其化学组成研究

EXTRACTION METHOD AND CHEMICAL COMPOSITION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM DIATOM ACHNANTHES JAVANICA VAR. SUBCONSTRICTA

摘要: 优化了爪哇曲壳藻亚缢变种(Achnanthes javanica var. subconstricta)胞外多聚物提取方法并分析了其化学组成。结果表明, 使用0.3 mol/L的碳酸氢钠提取时间为2.53h可充分提取非水溶性胞外多聚物而不会破碎细胞, 是最优的提取方法。爪哇曲壳藻亚缢变种胞外多聚物多糖占80%以上, 蛋白质占16%19%, 不含脂类。水溶性多糖的单糖组分摩尔比为: 岩藻糖:半乳糖:葡萄糖:甘露糖:木糖:鼠李糖=2.47:2.13:1:0.69:0.57:0.46,而非水溶性多糖的单糖组分摩尔比为: 半乳糖:岩藻糖:木糖:葡萄糖:甘露糖:鼠李糖=3.56:2.73:1.30:1:0.67:0.57。

Abstract: The current study reported the method to extract extracellular polymeric substances (EPSs) from Achnanthes javanica var. subconstricta and analyzed compositions of the EPSs. The results showed that the optimal extraction condition was 0.3 mol/L NaHCO3 with 2.53h extraction time, which produced little lysis. Polysaccharide is the main component of EPSs that contributed more than 80% of EPSs, while protein contributed 16%19% of EPSs. There is no lipid in the EPSs. The monosaccharide composition of flucose, galactose, glucose, mannose, xylose, and rhamnose in water-soluble polysaccharide is 2.47:2.13:1:0.69:0.57:0.46 (molar ratio). The monosaccharide composition of galactose, flucose, xylose, glucose, mannose, and rhamnose in non-water-soluble polysaccharide is 3.56:2.73:1.30:1:0.67:0.57.