池蝶蚌(贝)血细胞显微观察
MICROSCOPIC OBSERVING ON HEMOCYTES OF HYRIOPSIS SCHLEGELI
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摘要: 本文对池蝶蚌血细胞的形态结构及其动态变化进行了研究。根据血细胞形态、大小和密度等特征,把血细胞分为6类:颗粒细胞、透明细胞、浆液细胞、类淋巴细胞、梭形细胞和血栓细胞;并对各种血细胞显微结构予以描述,统计了血细胞胞体大小、细胞核大小、核质比、密度及所占比例,其中颗粒细胞所占比例最大,透明细胞次之,类淋巴细胞最少,颗粒细胞和透明细胞是两种主要的细胞类型,约占总数的82%,担负着最基本的代谢和免疫功能;通过对池蝶蚌血细胞形态的连续观察,发现血细胞存在形态变化现象,推测细胞间可能存在相互转化的情况。Abstract: Mussels do not possess an adaptive immune system as vertebrates do.The defence systems of mussels are often described as based only in innate immunity,which includes the cellular immunity and lymph immunity.A universal classification scheme of the circulating haemocytes is a prerequisite for comparative functional studies,but by now,it has been still not available for haemocytes of mussels.In hope of knowing about the defending system of mussel and try to improve the defense capability of the mussel,the classification and the dynamic variation of the mussel haemocytes were researched.The haemocytes of H.schlegeli which was stained purple by Giemsa were observed by light microscope.At the same time,the morphology of living haemocytes was also observed.The haemocytes were classified as 6 types based on the characteristics,amount of granule and its relative size,the nucleolus/plasm(N/P) ratio.They are granulocyte,hyalinocyte,serous cell,lymphoid cell,spindly cell and thrombocyte.Abundant big or small bright granules can be observed in the granulocyte which has the most number.The hyalinocytes were showed central nucleus surrounded by relatively lucent.Filopodia of the granulocyte and hyalinocyte stretched out from any direction.The size of granulocyte((18.43±2.29)μm) was larger than that of the hyalinocyte(13.19±1.87μm),but the N/C ratio of granulocyte(0.11±0.05) was lower than hyalinocyte(0.22±0.10).The lymphoid cell was the smallest((4.45±0.69)μm) with a relatively big nucleus which is(2.85±0.77)μm,and the serous cell was the biggest((19.41±2.57)μm).The folopodia of thrombocyte stretched out usually from one side of the cell body.The sizes and N/P ratio of the spindly cell were similar with the hyalinocyte and the thrombocyte.The percentage of the 6 kinds of haemocyte was counted.The average number of the hemolymph was(1.68±0.78)×106/mL,the granulocyte(45.84±2.20)%,the hyalinocyte(36.36±3.47)%,the serous cell(5.80±1.65)%,the lymphoid cell(2.77±1.27)%,the spindly cell(5.13±2.48)%,and the thrombocyte was about(4.09±2.07)%.Results indicate that the granulocyte and hyalinocyte may take the basal metabolism and immunological function.The dynamic variation in vivo of the haemocytes was observed under the LEICA DM5000 B Microscope,namely,the morphology of haemocytes underwent manifest changes by dipping them on the slides for a short time.The hemocytes just taken out from the shell were all round-shape,and 1-2 minutes later,most of them formed pesuclopods.After about 30 minutes,the pesuclopods withdraw and haemocytes became round.In this experiment,we found the hyalinocyte and the spindly cell can transform each other.The spindly cell transform to hyalinocyte was completed about 1 hour.The degranulation from one side of cell body was a voluntary and harmless process.When the granules moved out of haemocytes,they would be dissociative and actionless.All the haemocytes began to die and disintegrate within 1h to 1.5h.
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Keywords:
- Hyriopsis schlegeli /
- Haemocyte /
- Morphology /
- Dynamic variation
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[1] Liu S L, M aiK S. The progress of stud iesm ollusks Imm unolog icalsystem andm echan ism: a review. Ac taO ceanologica Sin ica, 2003,3, 25( 2 ): 95-105 [刘世良, 麦康森. 贝类免疫系统和机理的研究. 海洋学报, 2003, 3, 25( 2 ) : 95-105]
[2] P ipe R K, L ivngstone D R. An tioxidant en zym es associated w iththe b lood cells and haem olym ph of the mu sselMyritus edu lis [J]. F ish& Sh ellf ish Immun ology, 1993, 3: 221-233
[3] A llam B, A shton-A lcox K A, Ford S E. F low cytom etric com par-ison of h aem ocytes from th ree species of b iva lve m ollu sks [J].F ish& S hellf ish Imm unology, 2002, ( 13) : 141-58
[4] Sh iA J, Q iu A D, Sun Z Q. Ob servation of l iving hem ocyte offreshw ater pearlm usselAn od on taW ood iana E llip ticaH eud e [J].Journa l of S ichuan Un iversity ( Natu ral S cien ce Ed ition), 1999,36( 2 ): 332-336 [石安静, 邱安东, 孙志奇. 椭圆背角无齿蚌血细胞的活体观察. 四川大学学报( 自然科学版), 1999,36 ( 2) : 332-336]
[5] Shen Y P, M a L J. Prelim inary observations on haemocytes offreshw ater p earl oys ter CristariaP lica ta L ea ch [J]. A ctaH yd robiologica S in ica, 1993, 17( 2) : 190-192 [沈亦平, 马丽君. 淡水育珠蚌褶纹冠蚌血细胞的初步研究. 水生生物学报, 1993,17 ( 2) : 190-192]
[6] Y ang J, Sh iA J. Study on the class ification in hem ocytes of th reek ind of freshw ater p earl clam s [J]. Jou rnal of S ichuan Un iversity(N atural Scien ce Ed ition), 2002, 39 ( 1) : 68-72 [杨军, 石安静. 3 种淡水育珠蚌血细胞类型的研究. 四川大学学报(自然科学版), 2002, 39 ( 1) : 68-72]
[7] Xu M X, H ong Y J, et al. Prelim in ary study on d isease resistanceof pearl b ivalves (Hyriopsis schleg erli L. ) to disease-caus ingS sources of theH yriop sis Cum ing ii L. [J]. Journa l of N an changUn iversity (N aturalS cien ce), 2004, 28( 3) : 262-269 [徐毛喜,洪一江, 等. 池蝶蚌(Hyriopsi s schleg erli)对三角帆蚌病源的抗病性. 南昌大学学报(理科版), 2004, 28( 3 ): 262-269]
[8] ZhangW X, Wu X Z, et a l. Imm uno log ical funct ions of b lood cellsin th e scallopC hlamys farreri [J]. Ac ta Zoolog ica S in ica, 2005,51 ( 4) : 669-677 [张维翥, 吴信忠, 等. 栉孔扇贝血液细胞的免疫功能. 动物学报, 2005, 51 ( 4) : 669-677]
[9] X ing J, ZhanW B, et al. Determ ination of h em ocyte types and antibacterialactiv it ies for scallop (Ch lam ys farreri) [J]. Journa l ofOcean Un iversity of Q ingdao, 2003, 33( 1) : 41-46 [邢静, 占文斌, 等. 栉孔扇贝( Chlamys fa rreri )血细胞类型及抗菌力的研究. 青岛海洋大学学报, 2003, 33( 1 ) : 41-46]
[10] M a H M, M aiK S. Phagocytosis and non-self recogon it ion ofm o-lluscan b lood cells: a rev iew [J]. M arin e Sc iences, 2003, 27( 2 ):16-18 [马洪明, 麦康森. 贝类血细胞的吞噬作用和非我是别作用. 海洋科学, 2003, 27( 2 ) : 16-18]
[11] Ch en JH, B ayn e C J. B ivalve m ollu sc hem ocyte b ehaviors: character-ization of hem ocyte aggregat ion and adhes ion and their inh-ib ition in Californ ia m ussel (M ytilu s ca llf ornianu s ) [J]. B iolBu ll, 1995, 188: 255-266
[12] W ootton E C, Dyrynda E A, Ratcliffe N A. B ivalve immun ity:comparisons betw een th em arin em ussel (My tilu s edulis), the ed-ib le cock le (C erastoderm a edu le ) and th e razor-sh ell ( En sis siliqua) [J]. F ish& Shel lf ish Immunology, 2003, 15: 195-210
[13] M ixM C A. General m odel for leu cocyte cel l renew al in b ivalvem ollu sks [J].M ar. F ish Rew, 1976, 38: 37-41
[14] C ajarav ille M P, Pal S G, Rob ledo Y. The ligh t and electron m -icroscop ical localizat ion of lysosom al acid hydro lases in b ivalvehaemocyes by enzym e cytochem istry [J]. Ac ta H istochem Cy tochem,1995, 28 ( 5) : 409-416
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