鸭儿湖摇蚊复组种群酯酶多态性的研究
STUDIES ON THE ESTERASE POLYMORPHISM OF THE CHIRONOMUS COMPLEX POPULATION IN THE YA-ER LAKE NEAR WUHAN
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摘要: 应用淀粉凝胶电泳研究了从湖北鸭儿湖污染区采集的较耐污染的摇蚊复合组幼虫,结果发现:在取样种群中存在高活性的β酯酶(EST),其电泳迁移率与致倦库蚊实验参考系均不同;从甘油醛-3-磷酸脱氢酶(GPD)染色结果发现,其酶谱与已报道的库蚊GPD染色结果有很大不同,在污染最重的1号氧化塘的摇蚊复合组幼虫的GPD没有表达;但在中度污染的2、3号氧化塘中,摇蚊复合组幼虫GPD位点呈现明显多态现象.群体遗传学分析结果表明,取样种群由于杂合子不足造成种群偏离哈迪-温伯格平衡.所分析的2号、3号、4号塘三个亚种群之间遗传差异不显著,有效迁移个体数比较少,亚种群间基因流动少.Abstract: This paper dealed with the high resistant larvae of Chironomus complex which were collected from the Ya Er Lake near Wuhan. The results indicated that high active esterases existed in the population, the Rf is difference from conference population of Culex pipiens. The data of the GPD from Chironomus complex in four ponds indicated that the pattern of the electrophoresis is difference from the populations of Culex pipiens quinquefasciatus mosquitoes. In the middle polluted 2#, 3# pond, the GPD locus of the larvae of Chironomus complex demonstrated polymorphisms. The analysis results by GENEPOP software showed that the hypothesis of Hardy Weinberg equilibrium was rejected. There was systemic heterozygote deficit in these sub populations, no significant differentiation was found among the three sub populations 2#, 3#,4#, the gene flow among them was low according to the number of migrants of per generation(Nm).
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Keywords:
- Chironomus complex /
- Polymorphism /
- Telorance /
- Starch gel electrophoresis /
- Esterases
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Med Entomol, 1990, 27: 202-206[11] Pasteur N, Pasteur G, Catalan J, et al. Practical Isozyme Genetics [M]. Ellis Hrwood Lmd, Chister, England. 1988[12] Haldane J B C. An exact test for randomness of mating [J]. J Genet, 1954, 2: 631-635[13] Louis E J, Dempster E R. An exact test for Hardy-Weinberg and multiple alleles [J]. Biometrics, 1987, 43: 805-811[14] Guo S W, Thompson E A. Performing the exact test of Hardy-Weinberg proportions for multiple alleles [J]. Biometrics, 1992, 48: 361-372[15] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38:1358-1370[16] Fisher R A. Statistical methods for research workers(14th Edit)[M]. Edinbourgh: Olivier and Boyd. 1970[17] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38: 1358-1370[18] Wright S. Evolution and genetics of populations Vol.2. 1969. The theory of gene frequencies [M]. Chicago: Chicago University Press.[19] Nei M. Molecular evolutionary genetics [M]. New York: Columbia Univ press. 1987 Xu Y, Wu W Z.Mobility and transfer of residual Hexachlorocyclohexane(HCH)in aquatic environment [J]. Fresenius Envir. Bull, 1994, 3: 557-562[2] 翟启惠.昆虫分子生物学的一些进展:杀虫剂抗性的分子基础[J].昆虫学报,1995,38:493-500[3] Hemingway J, S H P P Karunaratne. Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechaniam [J]. Med. Vet. Entomol., 1998, 12: 1-12[4] Herath P R, Hemingway J. The detection and characterization of malathion resistance in filed population of Anopeles Culicifacies B in Sri Lanka Pestic [J]. Biochem. Physiol., 1987, 29: 157-162[5] Bisset J A, Rodriguez M M, Hemingway J, et al. Malathion and pyrethroid resistance in Culex quinquefasciatus from Cuba: efficacy pf primiphos-methyl in the presence of at laese three resistance mechanisms [J]. Med. Vet. Entomol., 1991, 5: 223-228[6] Karunaratne S H P P, Jayawardena K G I, Hemingway J, et al. Characterization of a B-type esterase involved in insecticide resistance from the mosquito Culex quinquefasciatus [J]. Biochem J, 1993, 294: 575-579[7] Raymond M, Qiao C L, Callaghan A, et al. Easterase polymorphism in insecticide susceptible populations of the mosquito Culex pipiens [J]. Genet Res Camb, 1996, 67: 19-26[8] Ranasinghe L E, Georghiou G P. Comparative modification of insecticide resistance spectrum of Culex fatigans Wied by selection with temephos and temephos/synergist combinations [J]. Pestic Science, 1979, 10: 502-508[9] Georghiou G P, Pasteur N, Hawley M K, et al. Linkage relationships between organophosphate resistance and a highly active esterase B in Culex pipiens quinquefasciatus Say from California [J]. J Econ Entomol, 1980, 73: 301-305[10] Wirth M C, Marquine M, Geoghiou G P, et al. Esterase A2 and B2 in Culex quinquefasciatus(Diptera: Culicidae): Role in organophoaphate resistance and linkage studies [J]. Med Entomol, 1990, 27: 202-206[11] Pasteur N, Pasteur G, Catalan J, et al. Practical Isozyme Genetics [M]. Ellis Hrwood Lmd, Chister, England. 1988[12] Haldane J B C. An exact test for randomness of mating [J]. J Genet, 1954, 2: 631-635[13] Louis E J, Dempster E R. An exact test for Hardy-Weinberg and multiple alleles [J]. Biometrics, 1987, 43: 805-811[14] Guo S W, Thompson E A. Performing the exact test of Hardy-Weinberg proportions for multiple alleles [J]. Biometrics, 1992, 48: 361-372[15] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38:1358-1370[16] Fisher R A. Statistical methods for research workers(14th Edit)[M]. Edinbourgh: Olivier and Boyd. 1970[17] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38: 1358-1370[18] Wright S. Evolution and genetics of populations Vol.2. 1969. The theory of gene frequencies [M]. Chicago: Chicago University Press.[19] Nei M. Molecular evolutionary genetics [M]. New York: Columbia Univ press. 1987
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