长臀鮠的AFLP分析
GENETIC ANALYSIS OF CRANOGLANIS BOUDERIUS BY MOLECULAR MARKER AFLP
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摘要: 采用AFLP分子标记技术对珠江长臀和海南长臀共60个个体(每个群体30个)进行了遗传多样性研究。选取的18对引物组合均能扩增出清晰、可重复的扩增产物,扩增带型差异明显。两个群体均表现出较高的多态位点比例和特异性条带数。群体内个体间的遗传相似度(平均值)珠江长臀种群为0.9462±0.0237,海南长臀为0.9465±0.0226,海南长臀比珠江长臀略高。群体间的遗传相似度是0.9367±0.0231,小于两个群体内的遗传相似度,两群体的遗传距离是0.0634±0.0230。根据遗传相似度绘制了UPGMA聚类图。研究结果表明:珠江长臀和海南长臀间的遗传差异属于种内差异,两者同属一个有效种。Abstract: The genus Cranoglanis is a group of fish only distributed in East Asia. They live in the drainages of Guangdong, Guangxi,Yunnan and Haiuan Province, China, and the Red River drainage in northern Vietnam. At present,the wild re-source of Cranoglanis bouderius has decreased rapidly because of excess utilized, and there are still some arguments on the validity of the species of the genus Cranoglanis. In order to determining the genetic structure and the validity of the spe-cies, AFLP technique was used to analyze the genetic diversity of Pearl River C. bouderias and Hainan C. bouderius. Sixty individuals from the two populations (thirty individuals per population) were analyzed by using eighteen primer combina-tions. The amplified bands of the eighteen primer combinations were in focus and repeated. The models of amplified band had distinct difference. The result of study showed that both populations had high percent of polymorphic loci and number of special bands. The genetic similarities was 0.9462?.0237 and 0.9465?.0226 within the population of Pearl River C. bouderius and the population of Hainan C. bouderius respectively, and was 0.9367?.0231 between Pearl River C. bouderius and Hainan C. bouderius, the genetic similarities between populations was lower than that within population. The genetic distance was 0.0634?.0230 between Pearl River C. bouderius and Hainan C. bouderius. The analyzed of phylo-genetic trees of two populations suggested that individuals of the same population combined together at first, and then two populations combined. The preliminary analysis result indicates that there are no prominent differences between genetic structure of Pearl River C. bouderius and Hainan C. bouderius, so they should be considered as one species.
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Keywords:
- Cranoglanis bouderius /
- AFLP /
- Genetic diversity
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