奥利亚罗非鱼与鳜杂交的受精细胞学及胚胎发育研究

唐永凯, 杨弘, 吴婷婷, 贾永义, 夏德全

唐永凯, 杨弘, 吴婷婷, 贾永义, 夏德全. 奥利亚罗非鱼与鳜杂交的受精细胞学及胚胎发育研究[J]. 水生生物学报, 2006, 30(5): 559-562.
引用本文: 唐永凯, 杨弘, 吴婷婷, 贾永义, 夏德全. 奥利亚罗非鱼与鳜杂交的受精细胞学及胚胎发育研究[J]. 水生生物学报, 2006, 30(5): 559-562.
TANG Yong Kai, YANG Hong, WU TingTing, JIA YongYi, XIA DeQuan. STUDY ON FERTILIZATION CYTOLOGY ANDEMBRYONICDEVELOPMENT IN HYBRIDIZATION BETWEEN OREOCHROMIS AUREA (♀) AND SINIPERCA CHUATSI (♂)[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(5): 559-562.
Citation: TANG Yong Kai, YANG Hong, WU TingTing, JIA YongYi, XIA DeQuan. STUDY ON FERTILIZATION CYTOLOGY ANDEMBRYONICDEVELOPMENT IN HYBRIDIZATION BETWEEN OREOCHROMIS AUREA (♀) AND SINIPERCA CHUATSI (♂)[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(5): 559-562.

奥利亚罗非鱼与鳜杂交的受精细胞学及胚胎发育研究

基金项目: 

国家自然科学基金项目(30371116)

国家“十五”攻关专题(2004BA526B0110)

“863”课题(2001AA243061)资助

STUDY ON FERTILIZATION CYTOLOGY ANDEMBRYONICDEVELOPMENT IN HYBRIDIZATION BETWEEN OREOCHROMIS AUREA (♀) AND SINIPERCA CHUATSI (♂)

  • 摘要: 通过对奥利亚罗非鱼(♀)和鳜(♂)杂交的受精细胞学观察发现:异源精子入卵后产生星光,并核化成雄性原核,与雌原核互相靠近,接触最终完全融合为一个合子核,合子核继续分裂。分析比较了奥利亚罗非鱼,鳜以及奥鳜杂种胚胎发育情况,发现杂种胚胎的发育速度在囊胚早期与母本奥利亚罗非鱼基本一致,到囊胚后期速度有所减慢,但与鳜胚胎发育速度相差很大。这表明奥利亚罗非鱼和鳜虽属不同科间的远缘杂交,但有正常的受精细胞学程序和常规的细胞分裂(卵裂)方式。
    Abstract: Tilapia are native to Africa, but have been introduced into many countries around the world.They are disease-resis-tant, reproduce easily, eat a wide variety of foods and tolerate poor water quality with low dissolved oxygen levels, but are not able to bear low temperature.Mandarin fish, a carnivore freshwater fish,is popular with the advantage of rich nutrition and deli-cious tasty. So we want to obtain a new breed through hybridization between Oreochromis aurea(♀) and Siniperca chuatsi(♂). And we finally got some progenies through this experiment. The insemination biology in hybridization between O. aurea(♀)and S.chuatsi(♂)reveals that the sperm of S.chuatsi can enter the egg cytoplasm of O. aurea, then grow into spermaster and finally form the male pronucleus.After a while, the fe-male and male pronucleus contact and fuse each other, and ultimately became zygote nucleus.Observing the embryonic development between the hybrid and its parents (O. aurea and S.chuatsi), we discover that the embryonic development rate of the hybrid is much closer in the early-blastula and is little slower in the late-blastula to that of O. aurea, but is considerably differentto that of S. chuatsi.Although the hybridization between O. aurea(♀)and S. chuatsi(♂)is distant hybridization, this study shows that the whole process obeys the normal fertilization process and cleavage.
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出版历程
  • 收稿日期:  2005-03-07
  • 修回日期:  2006-05-27
  • 发布日期:  2006-09-24

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