离心力对制备沉积物间隙水中化合物浓度的影响

朱江, 周永欣, 葛虹, 徐立铭, 张扬

朱江, 周永欣, 葛虹, 徐立铭, 张扬. 离心力对制备沉积物间隙水中化合物浓度的影响[J]. 水生生物学报, 2003, 27(5): 487-491.
引用本文: 朱江, 周永欣, 葛虹, 徐立铭, 张扬. 离心力对制备沉积物间隙水中化合物浓度的影响[J]. 水生生物学报, 2003, 27(5): 487-491.
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ZHU Jiang, ZHOU Yong-Xin, GE Hong, XU Li-Ming, ZHANG Yang. EFFECT OF CENTRIFUGAL FORCE ON COMPOUNDS CONCENTRATIONS IN SEDIMENT INTERSTITIAL WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 487-491.
Citation: ZHU Jiang, ZHOU Yong-Xin, GE Hong, XU Li-Ming, ZHANG Yang. EFFECT OF CENTRIFUGAL FORCE ON COMPOUNDS CONCENTRATIONS IN SEDIMENT INTERSTITIAL WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 487-491.
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离心力对制备沉积物间隙水中化合物浓度的影响

  • 中国地质大学工程学院, 武汉, 430074
    2. 中国科学院水生生物研究所, 武汉, 430072
    3. 湖北省水产科学研究所, 武汉, 430071
    4. 中国科学院武汉植物研究所, 武汉, 430074

基金项目: 

农业部渔业环境基金资助

长江渔业资源管理委员会基金资助

EFFECT OF CENTRIFUGAL FORCE ON COMPOUNDS CONCENTRATIONS IN SEDIMENT INTERSTITIAL WATER

  • School of Engineer, China University of Geoscience, Wuhan, 430074;
    2. Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072;
    3. Hubei Fisheries Sciences Research Institute, Wuhan, 430071;
    4. Wuhan Institute of Botany, the Chinese Academ, Wuhan, 430074

摘要

    摘要
  • 摘要: 研究了离心力对间隙水化合物浓度的影响.将腐殖质掺入东湖沉积物样品,按泥:水=1:4(体积)比室温下静置30d,随后掺入铜,泥:水=1:4(体积)比静置10d,得到总有机碳含量在1.47%-.72%、铜含量在120-2700mg/kg(干重)的试验沉积物样品,用离心法制备沉积物中的间隙水.离心参数为3000r/min、200r/min、9000r/min、1000r/min和12000r/min,4 ℃下离心20min.间隙水中化合物浓度分析表明,随离心力升高,间隙水中铜、铁、总有机碳(TOC)含量逐渐降低,钙的含量略有增加,而锰的含量不受离心力变化的影响.根据上述分析结果,确定在腐殖质对沉积物中铜的毒性影响研究中,制备沉积物间隙水的适宜离心条件为:12000r/min、4℃下离心20min.
    Abstract: Freshwater sediment from Eastlake, Wuhan, China, was spiked with humus and copper to obtain a series of different levels of total organic carbon (TOC, 1.47%—5.72%)and total copper (120—2700mg/kg dry weight). In laboratory, humus was added to natural freshwater sediment and stored at 20℃ for 30 days in a volume portion of 1:4 (sediment to water), then samples was spiked with copper and stored for 10 days. These humus and copper-spiked sediments were centrifuged under 3000r/min, 5200r/min, 9000r/min, 10500r/min, 12000r/min, 4℃ and 20min to collected its interstitial water (IW). chemica1 analysis showed that, Cu, Fe, TOC concentrations in IW decreased as centrifugal speed increased, meanwhile Ca somewhat increased but Mn concentration had no apparent correlation with centrifugal speed. According to the above results, the centrifugation method for interstitial water was preferable at: 12000r/min(9000g RCF), 4℃, 20min in study of humus effect on the toxicity of copper in sediment.
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  • [1] Hu W, Zhou Y X. The toxicity of copper-spiked sediment to Daphnia magna [J]. Acta Hydrobiologica sinica. 1995,19:82-86. [胡炜,周永欣. 铜污染沉积物对大型蚤的毒性研究. 水生生物学报,1995,19:82-86][2] Liu W X, Tang H X. Modification and optimization of current approaches for sediment qua1ity criteria [J]. China Environmental Scinence. 1997,17(3):220-224. [刘文新,汤鸿霄.区域沉积物质量基准常用建立方法的改进与优化. 中国环境科学,1997,17(3):220-224][3] Wang L X, Chen J S, Hong S. The new advance of sediment qua1ity criteria for heavy metals -The bio1ogica1 effect database-based approach [J]. Environmental Science and Technology. 2001,94:4-8. [王立新,陈静生,洪松. 水体沉积物重金属质量基准研究新进展-生物效应数据库法. 环境科学与技术,2001,94:4-8][4] Carignan R, Rapin F, Tessier A. Sediment porewater sampling for metal analysis: a comparison of techniques [J]. Geochem Cosmochim Acta, 1985,49:2493-2497[5] Schults D W, Ferraro S P, Smith L M, et al. A comparison of methods for collecting interstitial water for trace organic compounds and metal analysis [J]. Water Res., 1992, 26:989-995[6] Ank1ey G T, Schubauer-Berigan M K. Comparison of techniques for the isolation of sediment pore water for toxicity testing [J]. Arch. Environ. Contam. Toxicol., 1994,27:507-519[7] Poston T J, Purdy R,Aquatic Toxicology and Environmental Fate ASTM STP 921 [M], Philadelphia, American Society for Testing and Materials, 1986,479-493[8] Swartz R C, Kemp P F, Schults D W, et al. Acute toxicity of sediment from Eagle Harbor, Washington, to the infaunal Amphipod Rhepoxynius Abronius.[J]Environ Toxicol. Chem., 1989,8:215-222[9] Ankley G T, Katko A, Arthur J. Identificatiou of ammonia as an important sediment -associated toxicant in the lower Fox River and Green Bay, Wisconsin[J]. Environ. Toxicol. Chem., 1990,9:313-322[10] Sarda N, Burton G A. Ammonia variation in sediments: spatial, temporal and method-related effected [J]. Environ. Toxicol. Chem., 1995,14:1499-1506[11] Frazier B E, Naimo T J, Sandheinrich M B. Temporal and vertical distribution of tota1 ammonia nitrogen and un-ionized ammonia nitrogen in sediment pore water from the upper Mississippi River [J]. Environ. Toxciol. Chem., 1996,15:92-99[12] Burton C S. Developing of formulated reference sediments for freshwater and estuarine sediment testing [J]. Environ. Toxicol. Chem., 1994, 13:1163-1175[13] Adams D D, Darby D A, Young R J. Selected analytical techniques for characterizing the metal chemistry and geology of fine-grained sediments and interstitial water [A]. Robere A. Contaminants and sediment [C]. Arbor:Ann Arbor Sciences publishers. 1980

    Hu W, Zhou Y X. The toxicity of copper-spiked sediment to Daphnia magna [J]. Acta Hydrobiologica sinica. 1995,19:82-86. [胡炜,周永欣. 铜污染沉积物对大型蚤的毒性研究. 水生生物学报,1995,19:82-86][2] Liu W X, Tang H X. Modification and optimization of current approaches for sediment qua1ity criteria [J]. China Environmental Scinence. 1997,17(3):220-224. [刘文新,汤鸿霄.区域沉积物质量基准常用建立方法的改进与优化. 中国环境科学,1997,17(3):220-224][3] Wang L X, Chen J S, Hong S. The new advance of sediment qua1ity criteria for heavy metals -The bio1ogica1 effect database-based approach [J]. Environmental Science and Technology. 2001,94:4-8. [王立新,陈静生,洪松. 水体沉积物重金属质量基准研究新进展-生物效应数据库法. 环境科学与技术,2001,94:4-8][4] Carignan R, Rapin F, Tessier A. Sediment porewater sampling for metal analysis: a comparison of techniques [J]. Geochem Cosmochim Acta, 1985,49:2493-2497[5] Schults D W, Ferraro S P, Smith L M, et al. A comparison of methods for collecting interstitial water for trace organic compounds and metal analysis [J]. Water Res., 1992, 26:989-995[6] Ank1ey G T, Schubauer-Berigan M K. Comparison of techniques for the isolation of sediment pore water for toxicity testing [J]. Arch. Environ. Contam. Toxicol., 1994,27:507-519[7] Poston T J, Purdy R,Aquatic Toxicology and Environmental Fate ASTM STP 921 [M], Philadelphia, American Society for Testing and Materials, 1986,479-493[8] Swartz R C, Kemp P F, Schults D W, et al. Acute toxicity of sediment from Eagle Harbor, Washington, to the infaunal Amphipod Rhepoxynius Abronius.[J]Environ Toxicol. Chem., 1989,8:215-222[9] Ankley G T, Katko A, Arthur J. Identificatiou of ammonia as an important sediment -associated toxicant in the lower Fox River and Green Bay, Wisconsin[J]. Environ. Toxicol. Chem., 1990,9:313-322[10] Sarda N, Burton G A. Ammonia variation in sediments: spatial, temporal and method-related effected [J]. Environ. Toxicol. Chem., 1995,14:1499-1506[11] Frazier B E, Naimo T J, Sandheinrich M B. Temporal and vertical distribution of tota1 ammonia nitrogen and un-ionized ammonia nitrogen in sediment pore water from the upper Mississippi River [J]. Environ. Toxciol. Chem., 1996,15:92-99[12] Burton C S. Developing of formulated reference sediments for freshwater and estuarine sediment testing [J]. Environ. Toxicol. Chem., 1994, 13:1163-1175[13] Adams D D, Darby D A, Young R J. Selected analytical techniques for characterizing the metal chemistry and geology of fine-grained sediments and interstitial water [A]. Robere A. Contaminants and sediment [C]. Arbor:Ann Arbor Sciences publishers. 1980
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  • 收稿日期:  2002-11-12
  • 修回日期:  2002-12-29
  • 发布日期:  2003-09-24

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