镜湖萼花臂尾轮虫种群等位酶分析和生殖参数的季节变化
ALLOZYME ANALYSIS ON BRACHIONUS CALYCIFLORUS (ROTIFERA) POPULATION IN LAKE JINGHU AND SEASONAL VARIATION OF THEIR REPRODUCTIVE PARAMETERS
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摘要: 应用聚丙烯酰胺垂直平板凝胶电泳分析了镜湖萼花臂尾轮虫春季和夏季种群的葡萄糖磷酸异构酶(GPI)、苹果酸脱氢酶(MDH)和葡萄糖磷酸变位酶(PGM)三种酶系统,检测出GPI和MDH酶谱在两季节种群间存在着明显的差异;根据酶谱的不同可以把春季种群内的24个克隆划分为6个基因型不同的克隆群,夏季种群内的10个克隆划分为7个基因型不同的克隆群;春、夏两个季节种群内具有2个基因型相同的克隆群;各基因型在轮虫种群内的发生频率不尽相同。对轮虫两季节种群内共13个具有不同基因型的轮虫克隆在4个温度(15℃、20℃、25℃和30℃)下的种群增长率、种群中的混交雌体率和混交雌体受精率以及休眠卵产量等生殖参数所作的研究结果表明,各克隆的生殖参数对升高温度的反应不尽相同,温度和克隆以及两者间的交互作用对轮虫各生殖参数具有极显著的影响;这可能是镜湖萼花臂尾轮虫种群丰富的遗传变异得以维持的原因之一。上述结果显示,镜湖萼花臂尾轮虫种群不仅在生化遗传特征方面存在着显著的季节变异,在孤雌生殖速率(以种群增长率为指标)以及有性生殖的发生、过程和结局-混交雌体的产生、受精作用和休眠卵形成等生态特征方面还具有丰富的季节分化。Abstract: Three isozyme including gloucose phosphate isomerase(GPI),malica dehydrogenase(MDH) and phosphoglucomutase(PGM) were selected,and vertical polyacraylamide gel electrophoresis was used to screen 24 and 10 Brachious calyciflorus clones established respectively from the spring and summer collections from Lake Jinghu.Electrophoresis revealed three electromorph patterns for PGI and six ones for MDH in the clones from both spring and summer collections.Patterns of GPI and MDH enzymes revealed variations existed in the two seasonal populations.Six different clonal groups from the spring population and seven different ones from the summer population were identified by combing isozymes variations.Two clonal groups with same genotype existed in the spring and the summer populations.The frequency of the same genotype in the rotifer population differed with season,with that in the spring population being 0.0833,and the summer population 0.2.The variation in population structure of Brachious calyciflorus in Lake Jinghu from spring and summer could be described by overlap model(incomplete genetic discontinuity).The results of reproductive experiments made at 15℃,20℃,25℃ and 30℃ on thirteen clones representing different composite electromorphs in spring and summer collections showed that population growth rate,mictic rate,fertilization rate and resting egg production of rotifers varied from-0.054 to 1.443/d,0 to 0.88,0 to 1.0,0 to 129.7 ind./10mL·3d,respectively.The responses in population growth rate,mictic rate,fertilization rate and resting egg production of rotifers to increasing temperature were different among thirteen clones,and temperature,genotypes and their interactions all affected significantly population growth rate,mictic rate,fertilization rate and resting egg production,which might be one of the reasons that contribute to maintain the high genetic variations in the rotifer population.The results stated above indicated that the bio-chemically genetic characteristics of B.calyciflorus population in Lake Jinghu differed with season,and the ecological trait including rate of asexual reproduction(with the population growth rate as an endpoint),occurrence,course and result of sexual reproduction-formation and fertilization of mictic females,and resting egg production of B.calyciflorus population differentiated between the two seasons.
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Keywords:
- Brachionus calyciflorus /
- Seasonal population /
- Allozymes analysis /
- Temperature /
- Reproduction
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