Abstract: The chromosomes of Dugesia from different areas in China were researched via the method of squash.The results indi2 cate that the samples fromLuojia Hill of Wuhan City, Hubei Province are diploid with 16 chromosomes (2n = 2x = 16m), while samples from Tai Lake in Jiangsu Province have a triploid with 24 chromosomes (2n = 3x = 24m).Samples from Fenghuang Hill, Zunyi City, Guizhou Province have both the diploid (2n = 2x = 16m) and the triploid (2n = 3x = 24m) cells, i.e.They are sym2 phoid.This article gives the first2step researches of the classification and the ploidy of Dugesia fromthese three localities according to the results of the karyotype analysis.Besides, there are some relationships among the ploidy of the planarian, the generative mode and the temperature around during this research.