两个人工雌核发育红白锦鲤群体的RAPD标记分析
RAPD MARKER ANALYSIS OF TWO ARTIFICIAL GYNOGENETIC POPULATIONS IN RED-WHITE ORNAMENTAL CARP
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摘要: 采用RAPD方法,对两个人工雌核发育红白锦鲤群体进行了多态性及分子标记分析。结果表明,同一雌核发育群体具有基本一致的扩增产物,而不同雌核发育群体间的扩增产物则有较大不同;并从30个随机引物的扩增谱带中找到了7个引物(Opo-7,Opo-9,Opo-12,Opo-14,Opj-4,Opj-8和Opj-10)的扩增谱带可以作为两个不同雌核发育群体间的遗传标记。由UPGMA聚类法构建的分支系统树清晰地反映了两个雌核发育群体及其个体间的相互关系。Abstract: Genetic heterogeneity and molecular markers were analyzed in two artificial gynogenetic populations of Red-white ornamental carp (Cyprinus carpio L.) by RAPD technique, using 30 10-nucleotide primers. Similar banding patterns were observed among individuals within each gynogenetic population, whereas obvious differences were detected between the two gynogenetic populations. 7 primers, such as Opo-7, Opo-9, Opo-12, Opo-14, Opj-4, Opj-8 and Opj-10 were observed to produce specific bands, and these bands could be used as molecular markers for discriminating the two different gynogenetic populations. Average genetic distances within and among the two populations clearly indicated their origin and relationship. Aphylogenetic tree was constructed using UPGMA cluster analysis based on a total of 1710 distinguishable fragments (171 per individual). All individuals in the first gynogenetic population were clustered into one group, while all the individuals originated from the second were joined into another group.
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Keywords:
- Ornamental carp /
- Artificial gynogenesis /
- RAPD /
- Genetic marker
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[1] 桂建芳.单性脊椎动物的进化遗传学[J].自然杂志,1989,12(2):116-121[2] Oppermann K. Die entwicklung von forelleneiern nach Befruchtung mit radiumstrahlten Samenfaden [J]. Arch. Mikrosk. Anat. 1913,83:141-189[3] Pandian T J, Koteeswaran R. Ploidy induction and sex control in fish [J]. Hydrobiologia, 1998. 384:167-243[4] 吴清江,桂建芳.鱼类遗传育种工程[M].上海:上海科学技术出版社,1999[5] 桂建芳.锦鲤欣赏[J].水利渔业,1986,(5):40-42[6] 桂建芳,梁绍昌,孙建民.鱼类染色体组操作的研究Ⅰ、静水压休克诱导三倍体水晶彩鲫[J].水生生物学报.1990,14(4):336-344[7] 桂建芳,孙建民,梁绍昌,鱼类染色体组操作的研究Ⅱ、静水压处理和静水压与冷休克结合处理诱导水晶彩鲫四倍体[J].水生生物学报,1991,15(4):333-342[8] 杨书婷,桂建芳,两个雌核发育系白鲢群体同工酶分析及遗传标记的确定[J].水生生物学报,1999,23(3):264-268[9] 余先觉,周暾,李渝成,等.中国淡水鱼类染色体[M].北京:科学出版社.1989[10] 周莉,樊连春,桂建芳.银鲫复合种外源遗传物质整入的RAPD分析[J].水生生物学报,1998,22(4):301-306[11] Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases [J]. Proc. Natl. Acad. Sci. USA, 1985, 76:5269-5273[12] Zhou L, Wang Y, Gui J F. Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch based on detection of RAPD molecular markers [J]. Cytogenetics and Cell Genetics, 2000, 88:133-139[13] Tajima F. Evolutionary relationship of DNA sequences in finite populations [J]. Genetics, 1983, 105:437-445[14] Streisinger G, Walker C, Dower N, et al. Production of homozygous dipliod zebra fish [J]. Nature, 1981, 291:293-296[15] Carter R E, Mair, G C, Skibinski D O F, et al. The amplification of DNA fingerprinting in the analysis of gynogenesis in tilapia [J]. Aquaculture, 1991, 95:41-52[16] Corley-Smith G E, Lim G J, Brandhorst BP. Production of androgenetic Zebrafish (Danio rerio)[J]. Genetics, 1996, 142:1265-1278 桂建芳.单性脊椎动物的进化遗传学[J].自然杂志,1989,12(2):116-121[2] Oppermann K. Die entwicklung von forelleneiern nach Befruchtung mit radiumstrahlten Samenfaden [J]. Arch. Mikrosk. Anat. 1913,83:141-189[3] Pandian T J, Koteeswaran R. Ploidy induction and sex control in fish [J]. Hydrobiologia, 1998. 384:167-243[4] 吴清江,桂建芳.鱼类遗传育种工程[M].上海:上海科学技术出版社,1999[5] 桂建芳.锦鲤欣赏[J].水利渔业,1986,(5):40-42[6] 桂建芳,梁绍昌,孙建民.鱼类染色体组操作的研究Ⅰ、静水压休克诱导三倍体水晶彩鲫[J].水生生物学报.1990,14(4):336-344[7] 桂建芳,孙建民,梁绍昌,鱼类染色体组操作的研究Ⅱ、静水压处理和静水压与冷休克结合处理诱导水晶彩鲫四倍体[J].水生生物学报,1991,15(4):333-342[8] 杨书婷,桂建芳,两个雌核发育系白鲢群体同工酶分析及遗传标记的确定[J].水生生物学报,1999,23(3):264-268[9] 余先觉,周暾,李渝成,等.中国淡水鱼类染色体[M].北京:科学出版社.1989[10] 周莉,樊连春,桂建芳.银鲫复合种外源遗传物质整入的RAPD分析[J].水生生物学报,1998,22(4):301-306[11] Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases [J]. Proc. Natl. Acad. Sci. USA, 1985, 76:5269-5273[12] Zhou L, Wang Y, Gui J F. Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch based on detection of RAPD molecular markers [J]. Cytogenetics and Cell Genetics, 2000, 88:133-139[13] Tajima F. Evolutionary relationship of DNA sequences in finite populations [J]. Genetics, 1983, 105:437-445[14] Streisinger G, Walker C, Dower N, et al. Production of homozygous dipliod zebra fish [J]. Nature, 1981, 291:293-296[15] Carter R E, Mair, G C, Skibinski D O F, et al. The amplification of DNA fingerprinting in the analysis of gynogenesis in tilapia [J]. Aquaculture, 1991, 95:41-52[16] Corley-Smith G E, Lim G J, Brandhorst BP. Production of androgenetic Zebrafish (Danio rerio)[J]. Genetics, 1996, 142:1265-1278
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