Volume 36 Issue 6
Mar.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Ye Hua, Ren Peng, Liu Yang, Liu Xian-de, Wang Zhi-yong. ISOLATION AND GENETIC ANALYSIS OF MICROSATELLITE MARKERS FOR LARIMICHTHYS CROCEA[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(6): 1156-1163. DOI: 10.3724/SP.J.1035.2012.01156
Citation: Ye Hua, Ren Peng, Liu Yang, Liu Xian-de, Wang Zhi-yong. ISOLATION AND GENETIC ANALYSIS OF MICROSATELLITE MARKERS FOR LARIMICHTHYS CROCEA[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(6): 1156-1163. DOI: 10.3724/SP.J.1035.2012.01156
shu

ISOLATION AND GENETIC ANALYSIS OF MICROSATELLITE MARKERS FOR LARIMICHTHYS CROCEA

  • 1.

    Fisheries Breeding and Healthy Cultivation Research Centre, Southwest University, Chongqing 402460, China;

  • 2.

    Key Laboratory of Healthy Mariculture for East China Sea of Agriculture Ministry, PRC, Jimei University, Xiamen 361021, China

  • Received Date: April 18, 2012
  • Rev Recd Date: August 21, 2012
  • Published Date: November 24, 2012
    Graphical Abstract
    • Abstract
  • We constructed an (AC)n-microsatellite-enriched library for large yellow croaker Larimichthys crocea(Richardson 1846) using the method of FIASCO. Ninety clones were randomly selected for further colony amplification. The rate of positive clones reached to 66.7%, and 56 of the 60 positive clones sequences contained one or more SSR. By the analysis of repeat motif, dinucleotide repeats were the most dominant (91.1%), followed by trinucleotide repeats (8.9%). Among dinucleotides, (AC)n repeats were the most frequent (94.1%). Using Webers classification rules, the sequences were divided into three categories, the percentage of perfect repeat sequences, imperfect repeat sequences, and compound repeat sequences was 75.0%, 8.9%, and 16.1, respectively. Fifty two microsatellites primer pairs were designed and synthesized. The result showed that 35 SSRs were polymorphic in 46 F1 generation individuals. Twenty eight (80.0%) loci exhibited a segregation ratio of 1:1:1:1 (AB CD/AB AC), serving as the most useful markers segregating in co-dominant fashion. Six loci exhibited a segregation ratio of 1:1, and one loci exhibited a segregation of 1:2:1. Polymorphism analysis showed that 32 SSRs were consisted with Mendelian segregation ratio and could be used to construct the linkage map, while the other 3 SSRs (LYC0137, LYC0139, LYC0152) were departure from the expected Mendelian segregation patterns. These SSRs should be available for genetic linkage mapping, population genetics studies, molecular evolution and phylogenetic study of large yellow croaker.
    • FullText(HTML)
    • References (26)
  • [1]
    Su Y Q, Zhang C L, Wang J, et al. Breeding and Farming of Pseudosciaena crocea[M]. Beijing: Maritime Press. 2004, 1-7 [苏永全, 张彩兰, 王军, 等. 大黄鱼养殖. 北京: 海洋出版社. 2004, 1-7]
    [2]
    Wang Z Y, Xie F J, Cao M Y, et al. Aquaculture and Breeding of Large Yellow Croaker in China [R]. Texas, USA, Aquaculture. 2007, 973
    [3]
    Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers [J]. Nucleic Acids Research, 1989, 17(16): 6463-6471
    [4]
    Chistiakov D A, Hellemans B, Volckaert F A M. Microsatellites and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics [J]. Aquaculture, 2006, 255(1-4): 1-29
    [5]
    Liu Z J, Cordes J F. DNA marker technologies and their applications in aquaculture genetics [J]. Aquaculture, 2004, 238(1-4): 1-37
    [6]
    Ye X J, Wang Z Y, Liu X D, et al. Analysis of genetic homozygosity and diversity of two successive generation meio-gynogenetic population in Pseudosciaena crocea using microsatellite markers [J]. Acta Hydrobiologica Sinica, 2010, 34(1): 144-151 [叶小军, 王志勇, 刘贤德, 等. 大黄鱼连续两代雌核发育群体的微卫星标记分析. 水生生物学报, 2010, 34(1): 144-151]
    [7]
    Guo W, Wang Z, Wang Y, et al. Isolation and characterization of six microsatellite markers in the large yellow croaker (Pseudosciaena crocea Richardson) [J]. Molecular Ecology Notes, 2005, 5(2): 369-371
    [8]
    An H S, Cho K C, Park J Y. Eleven new highly polymorphic microsatellite loci in the yellow croaker, Pseudosciaena crocea[J]. Molecular Ecology Notes, 2005, 5(4): 866-868
    [9]
    Chang Y, Liang L, Lei Q, et al. Isolation and characterization of 11 microsatellite markers for the large yellow croaker, Pseudosciaena crocea[J]. Conservation Genetics, 2009, 10(5): 1405-1408
    [10]
    Hao J, Sun X W, Liang L Q, et al. Enrichment of large yellow croaker genome microsatellite markers using magnet beads [J]. Journal of Fishery Sciences of China, 2006, 13(5): 762-766 [郝君, 孙效文, 梁利群, 等. 大黄鱼微卫星标记的富集与筛选. 中国水产科学, 2006, 13(5): 762-766]
    [11]
    Ye H, Wang X Q, Gao T X, et al. EST-derived microsatellites in Pseudosciaena croceaand their applicability to related species [J]. Acta Oceanologica Sinica, 2010, 29(6): 83-91
    [12]
    Liu X D, Zhao G T, Wang Z Y, et al. Parentage assignment and parental contribution analysis in large yellow croaker (Larimichthys crocea) using microsatellite markers [J]. Current Zoology, 2012, 58(2): 244-249
    [13]
    Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed [M]. New York: Cold Spring Harbor Laboratory Press. 1989, 468-470
    [14]
    Zane L, Bargelloni L, Patarello T. Strategies for microsatellite isolation: a review [J]. Molecular Ecology, 2002, 11(1): 1-16
    [15]
    Wang Z Y, Tsoi K H, Chuk H. Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp [J]. Biochemical Systematics and Ecology, 2004, 32(4): 399-407
    [16]
    Weber J L. Informativeness of human (dc-da)n (dg-dt)n polymorphisms [J]. Genomics, 1990, 7(4): 524-530
    [17]
    Guo B Y, Xie C X, Qi P Z. et al. Construction and identification of DNA libraries enriched for microsatellite repeat sequences of Glyptosternum maculatum[J]. Acta Hydrobiologica Sinica, 2011, 35(6): 908-912 [郭宝英, 谢从新, 祈鹏志, 等. 黑斑原鮡微卫星DNA富集文库构建与鉴定. 水生生物学报, 2011, 35(6): 908-912]
    [18]
    Jerry D R, Evans B S, Matt K, et al. Development of a microsatellite DNA parentage marker suite for black tiger shrimp Penaeus monodon[J]. Aquaculture, 2006, 255(1-4): 542-547
    [19]
    Ren P, Wang Z Y, Yao C L, et al. Development of 11 polymorphic microsatellite loci in the small abalone (Haliotis diversicolor Reeve) [J]. Molecular Ecology Resources, 2008, 8(6): 1390-1392
    [20]
    Bernardi G, Mounchiroud D, Gautier C, et al. Compositional patterns in vertebrate genomes: Conservation and charge in evolution [J]. Molecular Evolution, 1988, 28(1-2): 7-18
    [21]
    Sueoka N. On the genetics basis of variation and heterogeneity of DNA base composition [J]. Proceedings of the National Academy of Sciences, USA, 1962, 48(4): 582-592
    [22]
    Toth G, Gaspari Z, Jurka J. Microsatellites in different eukaryotic genomes: survey and analysis [J]. Genome Research, 2000, 10(3): 967-981
    [23]
    Xu P S, Wang L, Liu E, et al. Generation of channel catfish BAC end sequences for marker development and assessment of syntenic conservation with other fish species [J]. Animal Genetics, 2006, 37(4): 321-326
    [24]
    Hauswaldt J S, Glenn T C. Microsatellite DNA loci from the diamondback terrapin (Malaclemys terrapin) [J]. Molecular Ecology Notes, 2003, 3(2): 174-176
    [25]
    Banks M A, Blouin M S, Baldwin B A, et al. Isolation and inheritance of novel microsatellites in Chinook salmon (Oncorhymchus tschawyscha) [J]. Journal of Heredity, 1999, 90(2): 281-288
    [26]
    Callen D F, Thimpson A D, Shen Y, et al. Incidence and origin of null alleles in the (AC)n microsatellite markers [J]. The American Journal of Human Genetics, 1993, 52(5): 922- 927
    • Related Articles

  • Related Articles

    [1]CHEN Kai, FANG Cheng-Chi, WU Zhi-Gang, XIONG Fan, YU Dan, CUI Yong-De, ZHANG Qi, WANG Bao-Qiang, JIANG Chuan-Qi, SONG Li-Rong, WANG Hong-Zhu, LIU Huan-Zhang, CHEN Xiao-Fei, LING Hai-Bo, CAI Jun-Xiong, LI Tao, HE Shun-Ping, MIAO Wei, XIONG Jie, ZENG Hong-Hui. AeDNA: AQUATIC ENVIRONMENTAL DNA DATABASE[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1741-1747. DOI: 10.7541/2022.2022.0379
    [2]XING Ying-Chun, GAO Wan-Ru, BAI Jie, ZHAO Ya-Hui. APPLICATIONS OF ENVIRONMENTAL DNA IN LAKE BIODIVERSITY[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(1): 137-148. DOI: 10.7541/2021.2020.237
    [3]WU Zhi-Gang, XIONG Wen, HOU Hong-Wei. BIODIVERSITY PATTERN AND CONSERVATION OF AQUATIC VASCULAR PLANTS IN THE YANGTZE RIVER BASIN, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(S1): 27-41. DOI: 10.7541/2019.164
    [4]WANG Jin-Wang, ZOU Ying-Ying, YU Dan. THE DIVERSITY, NICHE BREADTH, AND NICHE OVERLAP OF AQUATIC PLANTS IN THE OUJIANG RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(6): 1184-1197. DOI: 10.7541/2015.155
    [5]WANG Qiang, YUAN Xing-Zhong, LIU Hong. COMMUNITY AND BIODIVERSITY OF AQUATIC INSECTS ATTACHED ON THE STONES IN UPLAND HEADWATER STREAM OF SOUTHWESTERN CHINA: A CASE STUDY OF YUDU STREAM IN CHONGQING[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(5): 887-892. DOI: 10.3724/SP.J.1035.2011.00887
    [6]LI Zhong-Qiang, ZHANG Meng, XU Jun. BIODIVERSITY AND COMMUNITY ECOLOGY OF AQUATIC PLANT IN LAKE NAM CO,TIBETAN PLATEAU[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(1): 1-13. DOI: 10.3724/SP.J.1035.2011.006161
    [7]ZHENG LingLing, SONG LiRong, WU XingHua, ZHUANG HuiRu. ANALYSIS OFMORPHOLOGY AND 18S rDNA GENE FROM THE CAUSATIVE SPECIE RELATED DIATOM BLOOM IN HANJIANG RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 562-565.
    [8]DONG Zhi-Guo, LI Jia-Le. BIODIVERSITY AND CONSERVATION OF FRESHWATER MOLLUSKS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(4): 440-444.
    [9]SHI Wei gang, WANG Bo, WANG Li min. PRELIMINARY STUDIES ON THE TREND OF CHANGE IN BIODIVERSITY OF AQUATIC ANIMAL COMMUNITY IN THE LOWER REACHES OF THE CHANGJIANG RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(6): 654-661.
    [10]LI Zhi-gang, YANG Guan-pin, ZHU Yan-hong. THE RELATIONSHIP BETWEEN POLYMORPHISM OF BACTERIAL 16S rDNA RESTRICTION FRAGMENT LENGTH AND COMMUNITY STRUCTURE OF AQUATIC BACTERIA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 111-115.

  • Cited by

    Periodical cited type(6)

    1. 赵燕, 吕若萱, 操志晨, 朱波润, 张芯玮, 唐清美, 张秋胜. 口服型重组草鱼生长激素基因的创建及其原核表达最佳条件研究. 鲁东大学学报(自然科学版). 2020(01): 40-47 .
    2. 汪亚平, 何利波. 我国转基因鱼研制的历史回顾与展望. 生物工程学报. 2016(07): 851-860 .
    3. 关海红, 梁利群. 转基因鲤与普通鲤鱼精巢结构和发育的对比观察. 东北农业大学学报. 2013(03): 95-100+151 .
    4. 关海红, 梁利群. 转基因鲤性腺结构及繁殖性能的研究. 江苏农业科学. 2013(03): 191-194 .
    5. 苗田田, 赵金良, 苌建菊, 许淼洋. 外源生长激素对尼罗罗非鱼生长、骨骼肌纤维增生及肥大的影响. 农业生物技术学报. 2012(11): 1315-1320 .
    6. 刘春雷, 常玉梅, 梁利群, 徐丽华, 刘金亮, 闫学春. 饥饿对转基因鲤与野生鲤生长竞争和性腺发育的影响. 生态学报. 2010(21): 5975-5982 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (1166) PDF downloads (731) Cited by(10)
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return