RESEARCH ON THE COMBINED SPECIES BETWEEN <i>GRATELOUPIA CORYMBCLADIA</i> LI ET DING AND <i>G. SUBPECTINATA</i> HOLMES BASED ON THE MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES

MA Yue; BIAN Yao; WEN Xin; ZHANG Xin-Tao; WANG Hong-Wei

doi:10.7541/2021.2020.231

Volume 45 Issue 6

Dec. 2021

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ACTA HYDROBIOLOGICA SINICA > 2021 > 45(6): 1361-1370. > DOI: 10.7541/2021.2020.231

MA Yue, BIAN Yao, WEN Xin, ZHANG Xin-Tao, WANG Hong-Wei. RESEARCH ON THE COMBINED SPECIES BETWEEN GRATELOUPIA CORYMBCLADIA LI ET DING AND G. SUBPECTINATA HOLMES BASED ON THE MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1361-1370. DOI: 10.7541/2021.2020.231

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RESEARCH ON THE COMBINED SPECIES BETWEEN GRATELOUPIA CORYMBCLADIA LI ET DING AND G. SUBPECTINATA HOLMES BASED ON THE MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES

1.
College of Life Sciences, Liaoning Normal University, Dalian 116081, China
2.
The Key Laboratory of Plant Biotechnology of Liaoning Province, Liaoning Normal University, Dalian 116081, China

Funds: Supported by the National Natural Science Foundation of China (31200521 and 31570209)

Received Date: October 19, 2020
Rev Recd Date: April 20, 2021
Available Online: September 06, 2021
Published Date: November 14, 2021

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Abstract

Abstract

Grateloupia corymbcladia Li et Ding and G. subpectinata Holmes distributed in Nanji Island, Wenzhou City, Zhejiang Province (specimen of origin) were studied in detail using molecular-assisted alpha taxonomy (MAAT). The results showed that: (1)G. corymbcladia were upright, purple-red, cartilage and 15—30 cm height. The main branch was flat, and the end was elongated to a sub-flat shape with 2—5 mm wide. Branchlets and 1—2 pinnate branches were distributed on both sides of the main branch. The reproductive structure of the female gametophyte included carpogonial branch ampullae and auxiliary cell ampullae, which were composed of five cells and four cells (Grateloupia type), respectively. Mature tetrasporangia originated from inner cortex cells and split into cross. The above characteristics were consistent with G. subpectinata. (2)Under laboratory conditions, the female gametophytes of G. corymbcladia and G. subpectinata both produced and divided to form discoids. The spore development types were all “mediate discal type”. (3)Based on the results of rbcL and COⅠ gene sequence analysis, there were no pairwise divergence between the six samples of G. corymbcladia and the G. subpectinata. They all formed a single monophyletic subclade. These results indicate that G. corymbcladia and G. subpectinata were the same species, and G. corymbcladia was regarded as the synonym of G. subpectinata.

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RESEARCH ON THE COMBINED SPECIES BETWEEN GRATELOUPIA CORYMBCLADIA LI ET DING AND G. SUBPECTINATA HOLMES BASED ON THE MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES

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RESEARCH ON THE COMBINED SPECIES BETWEEN GRATELOUPIA CORYMBCLADIA LI ET DING AND G. SUBPECTINATA HOLMES BASED ON THE MORPHOLOGICAL OBSERVATIONS, EARLY DEVELOPMENT AND MOLECULAR ANALYSES

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