A NEW TWO-STEP METHOD ON CULTURING SPONTANEOUSLY FLOCCULATING MICROALGA <i>PARACHLORELLA KESSLERI</i>

ZHAO Yan; WANG Cheng; WANG Chao-Xia; WANG Tian-Qi

doi:10.7541/2019.024

Volume 43 Issue 1

Dec. 2018

Turn off MathJax

Article Contents

Abstract

References

ACTA HYDROBIOLOGICA SINICA > 2019 > 43(1): 196-204. > DOI: 10.7541/2019.024

ZHAO Yan, WANG Cheng, WANG Chao-Xia, WANG Tian-Qi. A NEW TWO-STEP METHOD ON CULTURING SPONTANEOUSLY FLOCCULATING MICROALGA PARACHLORELLA KESSLERI[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(1): 196-204. DOI: 10.7541/2019.024

Citation:

PDF (743 KB)

A NEW TWO-STEP METHOD ON CULTURING SPONTANEOUSLY FLOCCULATING MICROALGA PARACHLORELLA KESSLERI

College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China

Funds: Supported by the First-class Discipline Construction Project in Zhejiang Province 2017 (Food Science and Engineering 1110JYN6517001G); China National Natural Science Fund (31772100)

Received Date: January 29, 2018
Rev Recd Date: June 16, 2018
Available Online: October 11, 2018
Published Date: December 31, 2018

Graphical Abstract

Abstract

Abstract

A newly isolated spontaneously flocculating microalgae Parachlorella kessleri F01 was used as the material, and the algae sample in autotrophic cultivation of one-step culture method was used as the control group. A two-step culture method was designed in which glucose was supplemented for mixotrophic cultivation at stage Ⅰ, and nutritional limitation treatments were done at stage Ⅱ, and we studied their effects on the lipid accumulation and flocculation performance of the algae. The algae cell number was determined by a hemocytometer. Biomass was measured via dry weight, and the total lipid was quantified with staining method. The extracellular polymeric substances (EPS) components of algae were analyzed by three-dimensional fluorescence spectroscopy. The results showed that: (1) The optimum glucose concentration was 10 g/L at stage Ⅰ. The alga lipid productivity reached to 204.25 mg/L/d on the 10th harvesting day, which was 16.20 times of that in the control group, and the self-flocculation rate of alga cell was 96.1% after sitting for 12h, which has no significant difference with that in the control group. (2) On the basis of stage Ⅰ, alga cells were cultured for 1 day in stage Ⅱ with different elemental treatments. The alga lipid productivity were 242.64 mg/L/d and 227.61 mg/L/d in the low-glucose group and the low-glucose coupled with low-nitrogen group, respectively, increasing by 18.8% and 11.4% of those at stage Ⅰ. After 4 days cultivation, the alga lipid productions of the four treatment groups including low-glucose, no-glucose, low-nitrogen and the low-glucose coupled with low-nitrogen significantly increased compared with those in the control group and stage Ⅰ. The highest production reached to 3.08 g/L in the low-glucose and low-nitrogen co-treatment group, which was 23.69 times of that in the control group, and increased by 51.0% compared with that in stage Ⅰ. The self-flocculation rates in four treatment groups at stage Ⅱ were nearly above 85.0%, which could satisfy the harvesting requirements. (3) The content of protein tryptophan in alga EPS was positively related to the self-flocculation rate. Different culture treatments could change the protein tryptophan content in EPS of algae cells, and thus affect their flocculation performance. Self-flocculation Parachlorella kessleri F01 is an excellent potential alga for biodiesel production. The two-step culture method can greatly enhance its lipid production. The advantage of self-flocculation combined with two-step culture method is expected to be the key breakthrough to solve the bottleneck of biodiesel production technology of microalgae.
- Parachlorella kessleri,
- Two-step culture method,
- Self-flocculation ability,
- Lipid productivity

FullText(HTML)

References (29)

References

[1]	Chisti Y. Biodiesel from microalgae [J]. Biotechnology Advances, 2007, 25(3): 294—306 doi: 10.1016/j.biotechadv.2007.02.001
[2]	Scott S A, Davey M P, Dennis J S, et al. Biodiesel from algae: challenges and prospects [J]. Current Opinion in Biotechnology, 2010, 21(3): 277—286 doi: 10.1016/j.copbio.2010.03.005
[3]	Li Y T, Han D X, Hu G R, et al. Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii [J]. Biotechnology and Bioengineering, 2010, 107(2): 258—268 doi: 10.1002/bit.22807
[4]	Procházková G, Brányiková I, Zachleder V, et al. Effect of nutrient supply status on biomass composition of eukaryotic green microalgae [J]. Applied Phycology, 2014, 26(3): 1359—1377 doi: 10.1007/s10811-013-0154-9
[5]	黄冠华, 陈峰, 任庆功. 应用小球藻制备生物柴油. 太阳能学报, 2010, 31(9): 1085—1091 Huang G H, Chen F, Ren Q G. Research on biodiesel production from Chlorella vulgaris [J]. Acta Energiae Solaris Sinica, 2010, 31(9): 1085—1091
[6]	Xia L, Ge H M, Zhou X P, et al. Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15 [J]. Bioresource Technology, 2013, 144(3): 261—267
[7]	Mujtaba G, Wookjin C, Choul-Gyun L, et al. Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions [J]. Bioresource Technology, 2012, 123: 279—283 doi: 10.1016/j.biortech.2012.07.057
[8]	李小妹, 廖兴辉, 王明兹, 等. 两步培养法提高栅藻的生物量及油脂含量. 中国油脂, 2014, 39(5): 53—56 LI X M, Liao X H, Wang M Z, et al. Improvement of biomass and oil content of Scenedesmus sp. by two-step cultivation [J]. China Oils and Fats, 2014, 39(5): 53—56
[9]	Alicja P N, Andrzej B. The effect of natural and synthetic auxins on the growth, metabolite content and antioxidant response of green alga Chlorella vulgaris [J]. Plant Growth Regulation, 2014, 73(1): 57—66 doi: 10.1007/s10725-013-9867-7
[10]	Vitova M, Bisova K, Kawano S, et al. Accumulation of energy reserves in algae: from cell cycles to biotechnological applications [J]. Biotechnology Advances, 2015, 33: 1204—1218 doi: 10.1016/j.biotechadv.2015.04.012
[11]	Fernández S J M, Cerón G M C, Sánchez M A, et al. Pilot-plant-scale outdoor mixotrophic cultures of Phaeodactylum tricornutum using glycerol in vertical bubble column and airlift photobioreactors: studies in fed-batch mode [J]. Biotechnology Progress, 2004, 20: 728—736 doi: 10.1021/bp034344f
[12]	Liang Y, Sarkany N, Cui Y. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions [J]. Biotechnology Letters, 2009, 31(7): 1043—1049 doi: 10.1007/s10529-009-9975-7
[13]	Griffiths M J, van Hille R P, Harrison S T L. Lipid productivity, settling potential and fatty acid profile of 11 microalgal species grown under nitrogen replete and limited conditions [J]. Journal of Applied Phycology, 2012, 24(5): 989—1001 doi: 10.1007/s10811-011-9723-y
[14]	Zhou X, Ge H, Xia L, et al. Evaluation of oil-producing algae as potential biodiesel feedstock [J]. Bioresource Technology, 2013, 134: 24—29 doi: 10.1016/j.biortech.2013.02.008
[15]	Přibyl P, Cepák V, Zachleder V. Production of lipids in 10 strains of Chlorella and Parachlorella and enhanced lipid productivity in Chlorella vulgaris [J]. Applied Microbiology & Biotechnology, 2012, 94(2): 549—561
[16]	Uduman N, Qi Y, Danquah M K, et al. Dewatering of microalgal cultures: a major bottleneck to algae-based fuels [J]. Journal of Renewable and Sustainable Energy, 2010, 2(1): 12—17
[17]	Suali E, Sarbatly R. Conversion of microalgae to biofuel [J]. Renewable and Sustainable Energy Reviews, 2012, 16(6): 4316—4342 doi: 10.1016/j.rser.2012.03.047
[18]	Stanier R Y, Kunisawa R, Mandel M, et al. Purification and properties of unicellular blue-green algae (order chroococcales) [J]. Microbiology and Molecular Biology Reviews, 1971, 35: 171—205
[19]	Zhao P, Yu X Y, Li J J, et al. Enhancing lipid productivity by co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10 [J]. Journal of Bioscience and Bioengineering, 2014, 117(1): 72—77
[20]	任洁, 郎筱宇, 刘志媛. 三种脂染色法快速检测小球藻油脂相对含量. 农业生物技术学报, 2015, 23(7): 967—972 Ren J, Lang X Y, Liu Z Y. Rapidly determinating relative lipid level of Chlorella vulgaris sp. by three dyeing methods [J]. Journal of Agricultural Biotechnology, 2015, 23(7): 967—972
[21]	Alam M A, Wan C, Guo S L, et al. Characterization of the flocculating agent from the spontaneously flocculating microalga Chlorella vulgaris JSC-7 [J]. Journal of Bioscience and Bioengineering, 2014, 118(1): 29—33 doi: 10.1016/j.jbiosc.2013.12.021
[22]	Yang S F, Li X Y. Influences of extracellular polymeric substances (EPS) on the characteristics of activated sludge under non-steady-state conditions [J]. Process Biochemistry, 2009, 44: 91—96 doi: 10.1016/j.procbio.2008.09.010
[23]	Lv J P, Guo J Y, Feng J, et al. A comparative study on flocculating ability and growth potential of two microalgae in simulated secondary effluent [J]. Bioresource Technology, 2016, 205: 111—117 doi: 10.1016/j.biortech.2016.01.047
[24]	Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation-Emission matrix regional integration to quantify spectra for dissolved organic matter [J]. Environmental Science Technology, 2003, 37(24): 5701—5710 doi: 10.1021/es034354c
[25]	黄冠华, 陈峰, 魏东. 两步培养法提高蛋白核小球藻的油脂含量. 华南理工大学学报(自然科学版), 2008, 36(12): 97—101 doi: 10.3321/j.issn:1000-565X.2008.12.019 Huang G H, Chen F, Wei D. Improvement of lipid content of chlorella pyrenoidosa by two step cultivation [J]. Journal of South China University of Technology( Natural Science Edition ) , 2008, 36(12): 97—101 doi: 10.3321/j.issn:1000-565X.2008.12.019
[26]	Ho S H, Chen W M, Chang J S. Scenedesmus obliquus CNW-N as a potential candidate for CO₂ mitigation and biodiesel production [J]. Bioresource Technology, 2010, 101: 8725—8730 doi: 10.1016/j.biortech.2010.06.112
[27]	Li X L, Pribyl P, Bisova K, et al. The microalgaParachlorella kessleri-a novel highly efficient lipid producer [J]. Biotechnology and Bioengineering, 2013, 110(1): 97—107 doi: 10.1002/bit.24595
[28]	Fu L, Cui X C, Li Y B, et al. Excessive phosphorus enhances Chlorella regularis lipid production under nitrogen starvation stress during glucose heterotrophic cultivation [J]. Chemical Engineering Journal, 2017, 330: 566—572 doi: 10.1016/j.cej.2017.07.182
[29]	Fernandes B, Teixeira J, Dragone G, et al. Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the microalga Parachlorella kessleri [J]. Bioresource Technology, 2013, 144: 268—274 doi: 10.1016/j.biortech.2013.06.096

[1]	GAO Na, CHEN Cheng, ZHAO Xiu-Xia, FANG Ting, CUI Kai, LU Wen-Xuan. POLYPHASIC IDENTIFICATION AND WHOLE-GENOME ANALYSIS OF A SELF-FLOCCULATING BACTERIAL STRAIN, MASSILIA sp. W12[J]. ACTA HYDROBIOLOGICA SINICA, 2025, 49(2): 022517. DOI: 10.7541/2024.2024.0241
[2]	Ji-Shu ZHOU, Yan-Zi CAO, Hong JI, Hai-Bo YU. EFFECT OF DHA ON THE GROWTH PERFORMANCE AND LIPID METABOLISM IN TWO DIETARY LIPID LEVELS OF COMMON CARP, CYPRINUS CARPIO L.[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(1): 123-130. DOI: 10.7541/2018.016
[3]	GUO Qi, ZHENG Ling-Ling, SHEN Wei, SONG Li-Rong. EFFECTS OF DIFFERENT CO2 CONCENTRATION ON CO2 FIXATION AND LIPID ACCUMULATION OF TWO STRAINS OF SCENEDESMUS SP.[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(2): 414-418. DOI: 10.7541/2016.55
[4]	ZHANG Hu, ZHANG Gui-yan, WEN Xiao-bin, GENG Ya-hong, LI Ye-guang. EFFECTS OF PH ON THE PHOTOSYNTHESIS, GROWTH AND LIPID PRODUCTION OF CHLORELLA SP. XQ-200419[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(6): 1084-1091. DOI: 10.7541/2014.159
[5]	LI Zhong, LIANG Hong-Wei, ZOU Gui-Wei. A RAPID PCR-QUALITY DNA EXTRACTION METHOD IN FISH[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(2): 365-367. DOI: 10.3724/SP.J.1035.2012.00365
[6]	Shahid Mahboob, Bilal Hussain, Zahid Iqbal, Abdul Shakoor Chaudhry. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 484-491.
[7]	XIE Zhi-Gang, NIU Cui-Juan. EFFECTS OF PARTIAL AND COMPLETE FOOD DEPRIV ATION ON COMPENSATORY GROWTH OF JUVENILE SOFT -SHELLED TURTLE ( PELODISCUS SINENSIS):TEMPORAL PATTERNS IN GROWTH RA TE AND CHANGES IN BODY COMPOSITION[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 214-219.
[8]	YANG Wei-Dong, ZHANG Xin-Lian, LIU Jie-Sheng, GAO Jie, ZHANG Ping. INHIBITORY EFFECT AND SINKING BEHAVIOUR OF WOOD MEALS FROM CHINA FIR ON ALEXANDRIUM TAMARENSE IN CULTURES[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 215-219.
[9]	Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.
[10]	Liang Zhixin, Liang Jianyong, Chen Chao, Li Zhongjie, Lin Jinghong, Zhang Jianjun. THE EMBRYONIC DEVELOPMENT AND FINGERLING CULTURE OF LOACH, PARAMISGURNUS DABRYANUS SAUVAGE[J]. ACTA HYDROBIOLOGICA SINICA, 1988, 12(1): 27-42.

Cited By

Cited by

Periodical cited type(4)

1.	韩琪琪，张杰. 中药对水产动物免疫应答、抗氧化功能及抗病力影响的研究进展. 黑龙江畜牧兽医. 2024(22): 17-22 .
2.	马霞，周延州，郭振环，赵丽，刘永录，王林，张国祖. 玉屏风散与超微粉中毛蕊异黄酮葡萄糖苷体外溶出率的研究. 中国兽药杂志. 2019(11): 38-42 .
3.	黄小红，曹岩，江俊勇，马贵华，孔红兴，李达. 饲料中添加纳米硒对草鱼生长性能、免疫器官指数和抗氧化性能的影响. 中国饲料. 2017(16): 30-34 .
4.	巩华，刘春花，赵长臣，孙承文，黄志斌，陆文雄，龚钜财. 中草药预混料对罗非鱼生长性能和免疫抗病力的影响. 中国渔业质量与标准. 2017(02): 43-49 .

Other cited types(14)

Get Citation

PDF

XML

Article views (1810) PDF downloads (38) Cited by(18)

A NEW TWO-STEP METHOD ON CULTURING SPONTANEOUSLY FLOCCULATING MICROALGA PARACHLORELLA KESSLERI

Abstract

References

Related Articles

Cited by

Periodical cited type(4)

Other cited types(14)

Catalog

Related

A NEW TWO-STEP METHOD ON CULTURING SPONTANEOUSLY FLOCCULATING MICROALGA PARACHLORELLA KESSLERI

Abstract

References

Related Articles

Cited by

Periodical cited type(4)

Other cited types(14)

Catalog

Related

Export File

Citation

Format

Content