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QI Zhi-Tao, GAO Qian, HUANG Bei, CHANG Ming-Xian, NIE Pin. CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(5): 922-926.
Citation: QI Zhi-Tao, GAO Qian, HUANG Bei, CHANG Ming-Xian, NIE Pin. CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(5): 922-926.
shu

CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS

  • State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072;
    2. Department of Ocean Technology, Yancheng Institute of Technology, Key Laboratory of Aquaculture and Ecology of Coastal pool of Jiangsu Province, Yancheng 224051;
    3. Graduate University of the Chinese Academy of Sciences, Beijing 100049

  • Received Date: June 03, 2009
  • Rev Recd Date: March 17, 2010
  • Published Date: September 24, 2010
    Graphical Abstract
    • Abstract
  • A short type peptidoglycan recognition protein (PGRP-S) was first cloned from the amphibian model animal Xenopus tropicalis by using the bioinformatics method, and the expression pattern of xePGRP-S in early developing stage and normal xenopus was also analyzed. The xePGRP-S cDNA was 720 bp in length, containing a 549 bp open reading frame encoding 182 a.a. Multiple sequence alignment showed that the xePGRP-S shared 42.4%-50.5% sequence identities with other PGRP-S. RT-PCR showed that xePGRP-S mRNA was not detected in the early development stages (3-48h) until the third day. In the normal adult xenopus, xePGRP-S mRNA was constitutively expressed in all the detected tissues including heart, liver, spleen, lung, kidney, intestine and stomach.
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    • References (16)
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