CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS

A short type peptidoglycan recognition protein (PGRP-S) was first cloned from the amphibian model animal Xenopus tropicalis by using the bioinformatics method, and the expression pattern of xePGRP-S in early developing stage and normal xenopus was also analyzed. The xePGRP-S cDNA was 720 bp in length, containing a 549 bp open reading frame encoding 182 a.a. Multiple sequence alignment showed that the xePGRP-S shared 42.4%-50.5% sequence identities with other PGRP-S. RT-PCR showed that xePGRP-S mRNA was not detected in the early development stages (3-48h) until the third day. In the normal adult xenopus, xePGRP-S mRNA was constitutively expressed in all the detected tissues including heart, liver, spleen, lung, kidney, intestine and stomach.