ZHU Dong-Fa, WANG Gui-Zhong, LI Shao-Jing. ULTRASTRUCTURE OF OOGENESIS IN THENUS ORIENTALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(4): 439-445.
Citation: ZHU Dong-Fa, WANG Gui-Zhong, LI Shao-Jing. ULTRASTRUCTURE OF OOGENESIS IN THENUS ORIENTALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(4): 439-445.

ULTRASTRUCTURE OF OOGENESIS IN THENUS ORIENTALIS

  • Received Date: August 23, 2005
  • Rev Recd Date: November 06, 2005
  • Published Date: July 24, 2006
  • Oogenesis and oocyte vitellogenesis in the flathead lobster Thenus orientalis were observed in detail by transmission electron microscopy.According to the shape and size of ootids, ultrastructural changes of nucleus and cytoplasm, vitelline accumulation and the relationship between oocytes and follicle cells, the oogenesis of T.orientalis could be divided into four stages, i.e.oogonia, previtellogenic oocytes, vitellogenic oocytes and mature oocytes.Besides a large and oval nucleus, the oogonia mainly contain vesicles of smooth endoplasmic reticula.The nucleus of previtellogenic oocyte had becomemarkedly swollen, and was named specially as germinal vesicle.The nucleolar exudates appeared in the cytoplasm near out nuclear envelope of previte-l logenic oocyte.The oocyte in vitellogenic stage was surrounded progressively by follicle cells and formed a follicle.More and more yolk granules were formed and accumulated in the cytoplasm of vitellogenic oocyte.In T.orientalis, vitellogenesis appeared to involve both endogenous and exogenous yolk formation.The free ribosomes were the first organelles involved in the endogenous yolk formation.And the dense proteinous part icles synthesized by these free ribosomes could be accumulated and developed d-i rectly in ooplasm into yolk granules without membranes.The rough endoplasmic ret icula were the main organelles involved in the endogenous yolk formation.The smooth endoplasmic reticula took part in the endogenous yolk format ion by being transformed to rough endoplasmic reticula or fusing with rough endoplasmic reticula.By various ways, mitochondria and lysomes were involved act ively in the formation of yolk granules.In addition, golgi vesicles were also involved in the yolk format ion.There were two sources, the synthetic product of follicle cells and the precursor of lipovitellin carried by haemolymph, for exogenous materials accumulated in the perivitelline space.These exogenous materials were uptaken into oocytes by the micropinocytosis of plasmalemma and the absorption of microvilli, which were the two main ways of exogenous yolk formation.When oocyte became mature, ooplasm was nearly filled with yolk granules.Both endogenous and exogenous yolk materials were involved in the format ion of yolk granules with myelin figures in mature oocytes of T.orientalis.The formation of chorion in perivitelline space and the retraction of microvilli from chorion and perivitelline space could be regarded as the morphological markers of mature oocyte in T.orientalis.
  • [1]
    Krol R M, Hawkins W E, Overstreet R M. Reproduct ive components. In: HarrisonFW& HumesAG ed. Microscopic anatomy of invertebrates. Vol. 10. Decapod crustacea [M]. New York: Wiley-Liss, 1992. 295-343
    [2]
    Jiang, Y H, Yan S F, Song Z R. Ultrastructure of vitellogenesis in the oocytes of Litopenaeus vannamei [J]. Acta Zool. Sin., 2005, 51(1): 133-141 [姜永华, 颜素芬, 宋振荣. 凡纳滨对虾卵母 细胞卵黄发生的超微结构. 动物学报, 2005, 51(1): 133-141]
    [3]
    Wang Y F, DuN S, Lai W. Studies on the relat ionship of organelles in oocyte with vitellogenesis of Macrobrachium rosenbergii [J]. Acta Hydrobiol. Sin., 1999, 23(1): 24-28[王玉凤, 堵南山, 赖 伟. 罗氏沼虾卵母细胞细胞器与卵黄发生的关系. 水生生物学 报, 1999, 23(1): 24-28]
    [4]
    Yano I. Effect of 17A-hydroxyprogesterone on vitellogenin secretion in kurma prawn, Penaeus japonicus[J]. Aquaculture, 1987, 61: 49-57
    [5]
    Minagawa M, Sano M. Oogenesis and ovarian development cycle of the spiny lobster Panulirus japonicus (Decapoda: Palinuridae). Mar. Freshwater Res., 1997, 48: 875-887
    [6]
    Barnett B M, Hartwick R F, Milward N E. Phyllosoma and nisto stage of the morten bay bug, Thenus orientalis, from shelf waters of the Great Barrier Reef [J]. Aust. J. Mar. Freshwat. Res., 1984, 35 (2): 143-152
    [7]
    Hong S G, Lin JH, ChenX F, et al. Studies on oogenesis in prawn Penaeus peniciliatus I. Stages of oogenesis in Penaeus peniciliatus [J]. Oceanol. Limnol. Sin., 1988, 19(4): 301-306[洪水根, 林加涵, 陈细法, 等. 长毛对虾卵子发生的研究?. 卵子发生 过程. 海洋与湖沼, 1988, 19(4): 301-306]
    [8]
    Shangguan B M, Liu Z C, Li S J. Histological studies on ovarian development in Scylla serrata [J]. J. Fisheries China, 1991, 15(2): 96-103[上官步敏, 刘正琮, 李少菁. 锯缘青蟹卵巢发育的组 织学观察. 水产学报, 1991, 15(2): 96-103]
    [9]
    Gu Z M, HE L G. Histological and cytological observation on the development cycle of crab(Eriocheir sinensis-ovary[J]. Oceanol. Limnol. Sin., 1997, 28(2): 138-145[顾志敏, 何林岗. 中华绒螯 蟹卵巢发育周期的组织学细胞学观察. 海洋与湖沼, 1997, 28 (2): 138-145]
    [10]
    Wang G Z, Li S J, Zhu D F. Structures of ovary and ovarian follicle in flathead lobster, Thenus orientalis (Lund, 1793-(Crustacea: Decapoda: Scyllarida-[J]. Acta Oceanol. Sin., 2002, 24(5): 107)-114[王桂忠, 李少菁, 朱冬发. 东方扁虾卵巢和滤泡结构的研 究. 海洋学报, 2002, 24(5): 107-114]
    [11]
    itten-handed crab, Eriocheir sinensis (Crustacea, Decapoda-[J]. J. East ChinaNormal Univ. (Nat. Sci.), 1987, 3: 88-97[薛鲁征, 堵南山, 赖 伟. 中华绒螯蟹(Eriocheir sinensis-雌 性生殖系统的组织学研究. 华东师范大学学报(自然科学 版), 1987, 3: 88-97.

    Xue L Z, Du N S, Lai W. Histology of female reproduct ive system in Chinese mitten-handed crab, Eriocheir sinensis (Crustacea, Decapoda-[J]. J. East ChinaNormal Univ. (Nat. Sci.), 1987, 3: 88-97[薛鲁征, 堵南山, 赖 伟. 中华绒螯蟹(Eriocheir sinensis-雌 性生殖系统的组织学研究. 华东师范大学学报(自然科学 版), 1987, 3: 88-97]
    [12]
    Du N S, Lai W, Chen P C, et al. Studies on vitellogenesis of Eriocheir sinensis [J]. Acta Zool. Sin., 1999, 45(1): 88-92[堵南 山, 赖 伟, 陈鹏程, 等. 中华绒螯蟹卵黄形成的研究. 动物学 报, 1999, 45(1): 88-92]
    [13]
    Warner A H, Chu P P Y, ShawM F, et al. Yolk platelets in artemia embryos: are they really storage sites of immature mitochondria? [J]. Comp. Biochem. Physiol. B, 2002, 132: 491-503
    [14]
    Hinsch GW, ConeM V. Ultrastructural observat ions of vitellogenesis in the spider crab, Libinia emarginata L. [J]. J. Cell Biol., 1969, 40: 336-342
    [15]
    Tseng D Y, Chen Y N, Kou G H, et al. Hepatopancreas is the extraovarian site of vitellogenin synthesis in black tiger shrimp, Penaeus monodon [J]. Comp. Biochem. Physiol. A, 2001, 129: 909-917
    [16]
    Quackenbush L S. Yolk product ion in the marine shrimp Penaeus vannamei [J]. J. Crustacean Biol., 1989, 9(4): 509-516
    [17]
    Vazquez Boucard C G, Levy P, Ceccaldi H J, et al. Developmental changes in concentrations of vitellin, vitellogenin, and lipid in hemolymph, hepatopancreas, and ovaries from different ovarian stages of Indian white prawn Fenneropenaeus indicus [J]. J. Exp. Mar. Biol. Ecol., 2002, 281: 63-75
    [18]
    Talbot P. The ovary of the lobster, Homarus americanus ò. Structure of the mature follicle and origin of the chorion [J]. J. Ultrastruct. Res., 1981, 76: 249-262
    [19]
    Lee T H, Yamazaki F. Cytological observations on fertilizat ion in the Chinese freshwater crab, Eriocheir sinensis, by artificial insemination (in vit ro-and incubation [J]. Aquaculture, 1989, 76: 347-360
    [20]
    Kang X J, Wang S A, Du N S, et al. Cytological changes of sperm and egg nuclei during fertilization in Penaeus chinensis [J]. Acta Zool. Sin., 2001, 47(2): 182-189[康现江, 王所安, 堵南山, 等. 中国对虾受精过程中精卵核的细胞学变化. 动物学报, 2001, 47(2): 182-189]
    [21]
    Zhu D F, Wang C L, YuH W. Oogenesis, oocyte act ivation and early cleavage of Portunus trituberculatus (Miers-[J]. Oceanol. L imnol. Sin., 2005, 36(5): 423-429[朱冬发, 王春琳, 余红卫. 三疣梭子蟹(Portunus trituberculatus-卵子发生、激活与早期卵 裂的细胞学观察. 海洋与湖沼, 2005, 36(5): 423-429]

Catalog

    Article views (1057) PDF downloads (513) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return