PAN Hui, SONG Li-rong, LIU Yong-ding, ZHU Yun-zhi, SHEN Qiang. CHARACTERIZATION OF TOXIC WATERBLOOM-FORMING CYANOBACTERIA BY MODIFIED PCR[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 159-166.
Citation: PAN Hui, SONG Li-rong, LIU Yong-ding, ZHU Yun-zhi, SHEN Qiang. CHARACTERIZATION OF TOXIC WATERBLOOM-FORMING CYANOBACTERIA BY MODIFIED PCR[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 159-166.

CHARACTERIZATION OF TOXIC WATERBLOOM-FORMING CYANOBACTERIA BY MODIFIED PCR

  • Received Date: September 17, 2000
  • Rev Recd Date: October 23, 2000
  • Published Date: March 24, 2001
  • The present study surveyed the distribution of microcystin-producing gene-mcyB by PCR among 38 bloom-forming cyanobacterial strains from FACHB-Collection. The results demonstrated that special amplifications were being gotten only from 19 toxic Microcystis strains, whereas it was deficient in nontoxic ones got none. The PCR results showed highly agreement with the toxicity of every strain determined by HPLC, ELISA, and Bioassay respectively. Furthermore, the traditional PCR protocols were simplified by checking the cyanobacteria cells directly instead of the previously used extracted genomic DNA. The whole cells PCR we developed in this study could be successfully applied to cultured algae materials, water samples and lyophilized cyanobacterial cells. Thus, discrimination of toxic and nontoxic Microcystis strains by molecular biological technologies proves to be practicable and efficacious.
  • [1]
    Watanabe M F,Harada K,Carmichael W W,et al.Toxic Microcystis [M].New York:Boca Raton CRC Press 1996[2] Carmichael W W.The Toxins of Cyanobacteria [J].Scientific American.1994,274(1):78—86[3] Carmichael W W.Cyanobacterial Toxins [J].IOC Manuals and Guides.1996,33:163—175[4] Watanabe M F.,Oishi S.Toxicities of Microcystis aeruginosa collected from some lakes,reservoirs,ponds and moat in Tokyo and adjacent regions [J].Jpn.J.Limnol.1980,41:5—9[5] Shirai M K,Obotake M A.Toxicity and delayed type hypersensitivity caused by Microcystis blooms from lake Kasumigaura [J].Micrbiol Immunol.1986,30:731—735[6] Kato T,Watanabe M F,Watanabe M.Allozyme divergence in Microcystis(Cyanophyceae)and its taxonomic inference [J].Arch.Hyrobiol..Bd.1991,64(Suppl):129—140[7] Makoto S,Akio O,Takaki S,Seiishiro T.Toxicity and toxins of natural blooms and isolated strains of Microcystis sp.(Cyanobacteria)and improved procedure for purification of cultures [J].Applied and Environmental Microbiology.1991,4:1241—1245[8] Baker D.,Humpage A R.,Steffensen D A.Cyanobacterial blooms in the Murray-Basin:Their Taxonomy and Toxicity [M].Austr.Center for Water Quality Res.1993[9] Codd J N,Bettie K A,Lawton L A,et al.Blue-green algal toxins [J].British Phycol.1993,27:87[10] Dittmann E,Kathrin M,Borner T.Conserved sequences of pepide synthetase genes in the cyanobacterium Microcystis aeruginosa [J].Phycologia.1996,35(Suppl.):62—67[11] Kathrin M,Dittmann E,Borner T.Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa sequences homologous to peptide synthetase genes [J].FEMS Microbiology Letters.1996,135:295—303[12] Dittmann E,Neilan B A,Borner T.Insertional mutagenesis of a peptide synthetase gene that responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806 [J].Mol.Microbiol.1997,126(4):779—787[13] Rohrlack T,Dittmann E,Henning M,et al.Role of microcystins in poisoning and food ingestion inhibition Daphnia galeata caused by the cyanobacterium Microcystis aeruginosa [J].Applied and Environmental Microbiology.1999,65(2):737—739[14] Neilan B A,Dittmann E,Borner T.Nonribosomal peptide synthesis and toxigenicity of cyanobacteria [J].J.Bacteriol.1999,181,13:4089—4097[15] Dittmann E,Neilan B A,Borner T.Peptide synthetase genes occur in various species of cyanobacteria [M].The Phototrophic Prokaryotes.New York:Kluwer Academic/Plenum Publishers 1999[16] Ichimura T.Media for blue-green algae [M].Methods in algalogical studies.Tokyo,Kyoritsu,1979[17] 董传辉,俞顺章,陈 刚.某湖周围水厂源水及出厂水微囊藻毒素调查[J].卫生研究.1998,27,3:100—102[18] 宋立荣,雷腊梅,何振荣.滇池水华蓝藻铜绿微囊藻和绿色微囊藻的生长生理特性及毒素分析[J].水生生物学报.1999,23(5):402—407[19] 何家菀,李络平,俞家禄,等.我国产毒微囊藻的新发现——韦氏微囊藻及其毒性的初步研究[J].水生生物学报.1996,20,2:192—194

    Watanabe M F,Harada K,Carmichael W W,et al.Toxic Microcystis [M].New York:Boca Raton CRC Press 1996[2] Carmichael W W.The Toxins of Cyanobacteria [J].Scientific American.1994,274(1):78—86[3] Carmichael W W.Cyanobacterial Toxins [J].IOC Manuals and Guides.1996,33:163—175[4] Watanabe M F.,Oishi S.Toxicities of Microcystis aeruginosa collected from some lakes,reservoirs,ponds and moat in Tokyo and adjacent regions [J].Jpn.J.Limnol.1980,41:5—9[5] Shirai M K,Obotake M A.Toxicity and delayed type hypersensitivity caused by Microcystis blooms from lake Kasumigaura [J].Micrbiol Immunol.1986,30:731—735[6] Kato T,Watanabe M F,Watanabe M.Allozyme divergence in Microcystis(Cyanophyceae)and its taxonomic inference [J].Arch.Hyrobiol..Bd.1991,64(Suppl):129—140[7] Makoto S,Akio O,Takaki S,Seiishiro T.Toxicity and toxins of natural blooms and isolated strains of Microcystis sp.(Cyanobacteria)and improved procedure for purification of cultures [J].Applied and Environmental Microbiology.1991,4:1241—1245[8] Baker D.,Humpage A R.,Steffensen D A.Cyanobacterial blooms in the Murray-Basin:Their Taxonomy and Toxicity [M].Austr.Center for Water Quality Res.1993[9] Codd J N,Bettie K A,Lawton L A,et al.Blue-green algal toxins [J].British Phycol.1993,27:87[10] Dittmann E,Kathrin M,Borner T.Conserved sequences of pepide synthetase genes in the cyanobacterium Microcystis aeruginosa [J].Phycologia.1996,35(Suppl.):62—67[11] Kathrin M,Dittmann E,Borner T.Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa sequences homologous to peptide synthetase genes [J].FEMS Microbiology Letters.1996,135:295—303[12] Dittmann E,Neilan B A,Borner T.Insertional mutagenesis of a peptide synthetase gene that responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806 [J].Mol.Microbiol.1997,126(4):779—787[13] Rohrlack T,Dittmann E,Henning M,et al.Role of microcystins in poisoning and food ingestion inhibition Daphnia galeata caused by the cyanobacterium Microcystis aeruginosa [J].Applied and Environmental Microbiology.1999,65(2):737—739[14] Neilan B A,Dittmann E,Borner T.Nonribosomal peptide synthesis and toxigenicity of cyanobacteria [J].J.Bacteriol.1999,181,13:4089—4097[15] Dittmann E,Neilan B A,Borner T.Peptide synthetase genes occur in various species of cyanobacteria [M].The Phototrophic Prokaryotes.New York:Kluwer Academic/Plenum Publishers 1999[16] Ichimura T.Media for blue-green algae [M].Methods in algalogical studies.Tokyo,Kyoritsu,1979[17] 董传辉,俞顺章,陈 刚.某湖周围水厂源水及出厂水微囊藻毒素调查[J].卫生研究.1998,27,3:100—102[18] 宋立荣,雷腊梅,何振荣.滇池水华蓝藻铜绿微囊藻和绿色微囊藻的生长生理特性及毒素分析[J].水生生物学报.1999,23(5):402—407[19] 何家菀,李络平,俞家禄,等.我国产毒微囊藻的新发现——韦氏微囊藻及其毒性的初步研究[J].水生生物学报.1996,20,2:192—194
  • Related Articles

    [1]CHEN Jia-Xin, CAI Fang-Fang. ALLOCOLEOPSIS (COLEOFASCICULALES, CYANOBACTERIA), A NEW RECORD GENUS OF TERRESTRIAL CYANOBACTERIA IN CHINA[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2025.2024.0323
    [2]WANG Fei, ZENG Xiao-Li, ZHANG Cheng-Cai. IRON STRESS ON THE EXPRESSION OF MICROCYSTIN SYNTHETASE MCYC AND MCYI[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(7): 1036-1041. DOI: 10.7541/2023.2022.0217
    [3]ZHAO Shuang, CHEN Wei, JIA Yun-Lu, ZHENG Ling-Ling, ZUO Yan-Xia, SONG Li-Rong. CHARACTERISTICS OF MICROCYSTIN-DEGRADING BACTERIUM ISOLATED FROM SEDIMENTATION PONDS FOR THE TREATMENT OF CYANOBACTERIAL BLOOMS[J]. ACTA HYDROBIOLOGICA SINICA, 2013, 37(3): 522-529. DOI: 10.7541/2013.55
    [4]XU Xiao-Yan, QIAN Hai-Feng, CHEN Wei, JIANG Hong, FU Zheng-Wei. ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(1): 139-143.
    [5]WANG Xiu-Min, SHAO Min-Hua, LU Da-Ru, XU Li-Hong, YU Ying-Nian. USING HIGH2THROUGHPUT REAL TIME FLUORESCENCE QUANTITATIVE PCR TO SELECT RESPONSE GENES OF FL CELL EXPOSED TO MICROCYSTIN LR[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(6): 909-911.
    [6]ZHAI Chao, OU Dan-Yun, WU Zhong-Xing, ZHU Yun-Zhi, SONG Li-Rong. IDENTIFICATION OF TOXIC AND NON-TOXIC MICROCYSTIS BY THREE PRIMERS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(2): 119-123.
    [7]HU Yong, KONG Dong-Dong, HE Yi-Kun. ON-LINE CLONING AND EVOLUTION ANALYSIS OFCrMinD GENE FROMCHLAMYDOMONA SREINHARDTII[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 106-109.
    [8]XU Xu-dong, KONG Ren-qiu, LIU Ke, WANG Jiang-xin, NING De-gang. PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 544-550.
    [9]Chen Yueqin, He Jiawan, Zhuang Li, Zeng Longmei, Qu Lianghu. SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(1): 41-46.
    [10]Li Shaorong, Lin Huimin. PURIFICATION AND CHARACTERIZATION OF PHYCOERYTHRIN FROM RHODOSORUS MARINUS[J]. ACTA HYDROBIOLOGICA SINICA, 1996, 20(3): 257-264.

Catalog

    Article views (778) PDF downloads (453) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return