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WANG Lin, LI Bing, YU Jia-Hui, ZHU Jia-Bin, ZHU Jian. THE COMPARATIVE ANALYSIS OF THE SUBSTRATE ENZYME ACTIVITY AND WATER PURIFICATION EFFECT IN DIFFERENT WETLAND MICROCOSM[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(3): 692-699. DOI: 10.7541/2017.87
Citation: WANG Lin, LI Bing, YU Jia-Hui, ZHU Jia-Bin, ZHU Jian. THE COMPARATIVE ANALYSIS OF THE SUBSTRATE ENZYME ACTIVITY AND WATER PURIFICATION EFFECT IN DIFFERENT WETLAND MICROCOSM[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(3): 692-699. DOI: 10.7541/2017.87
shu

THE COMPARATIVE ANALYSIS OF THE SUBSTRATE ENZYME ACTIVITY AND WATER PURIFICATION EFFECT IN DIFFERENT WETLAND MICROCOSM

  • 1.

    Wuxi Fisheries College Nanjing Agricultural University, Wuxi 214081, China

  • 2.

    Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Wuxi 214081, China

Funds: Supported by the National “Twelfth Five-Year” Plan for Science & Technology Support (2012BAD25B07); National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS (2015JBFM24); the Projects of National Technology System for Conventional Freshwater Fish Industries (nyeytx-46)
  • Received Date: June 05, 2016
  • Rev Recd Date: July 26, 2016
  • Available Online: April 30, 2017
  • Published Date: April 30, 2017
    Graphical Abstract
    • Abstract
  • The aim of current research was to determine the most efficient model for water purification, by studying different combinations of plants and matrixes and related enzyme activities (urease and phosphatase) in small wetland models. Two types of plants: Zizania latifolia and Sagittaria graminea, and two types of matrixes: garnet and magnetite were used to construct six different wetland models. Within a wetland system, the quality of inflow and outflow water and the matrix enzyme activities were measured. The results indicated the wetland models have the highest removal rate of nitrite nitrogen, ranging between 98.87% and 58.06%, and the lowest removal rate of CODMn, ranging between 37.91% and 2.51%. Compared with the single plantation model, combination of both plant species yielded a synergy effect in improving water quality, evident by the increased average removal rates of total nitrogen (by 3%), ammonia nitrogen (by 10%), nitrite (10%), total phosphorus (15%), orthophosphate (20%), and CODMn (8%). This effect was independent of the use of matrix. The filter material urease activity was highly correlated with the total nitrogen removal rate (r2: 0.903—0.980), whereas phosphatase activity was highly correlated with the CODMn removal rate (r2: 0.821—0.992). Hence, urease and phosphatase can be used as predictors of the total content of nitrogen and CODMn, respectively, in a wetland system. This research provides a theoretical basis in optimal selection of wetland plants and matrixes and in evaluation of water purification effect by assessing substrate enzyme activities.
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  • [1]
    梁威, 吴振斌, 周巧红, 等. 复合垂直流构建湿地植物根区磷酸酶及脲酶活性与污水净化的关系. 植物生理学通讯, 2002, 38(6): 545— 548 http://www.cnki.com.cn/Article/CJFDTOTAL-ZWSL200206001.htm

    Liang W, Wu Z B, Zhou Q H, et al. Relationship between the phosphatase and urease activities in plant root-zone and purification of wastewater in the integrated vertical constructed wetland [J]. Plant Physiology Communications, 2002, 38(6): 545—548
    梁威, 吴振斌, 周巧红, 等. 复合垂直流构建湿地植物根区磷酸酶及脲酶活性与污水净化的关系. 植物生理学通讯, 2002, 38(6): 545— 548 http://www.cnki.com.cn/Article/CJFDTOTAL-ZWSL200206001.htm
    [2]
    李共国, 屠霄霞, 王佩儿, 等. 淡水排放对杭州湾湿地浮游动物群落分布的影响. 水生生物学报, 2013, 37(3): 397—404 doi: 10.7541/2013.35

    Li G G, TU X X, Wang P E, et al. Influence of freshwater discharge on zooplankt community distribution in the mudflat wetlands of the hangzhou bay [J]. Acta Hydrobiologica Sinica, 2013, 37(3): 397—404
    李共国, 屠霄霞, 王佩儿, 等. 淡水排放对杭州湾湿地浮游动物群落分布的影响. 水生生物学报, 2013, 37(3): 397—404 doi: 10.7541/2013.35
    [3]
    刘文生, 肖建光, 林齐鹏, 等. 人工湿地对胡子鲇养殖水体循环净化的研究 (英文). 水生生物学报, 2008, 32(1): 33—37 http://ssswxb.ihb.ac.cn/CN/abstract/abstract3473.shtml

    Liu W S, Xiao J G, Lin Q P, et al. Using planted constructed wetlands to treat wastewaters from circulating aquaculture of Clarias fuscus [J]. Acta Hydrobiologica Sinica, 2008, 32(1): 33—37
    刘文生, 肖建光, 林齐鹏, 等. 人工湿地对胡子鲇养殖水体循环净化的研究 (英文). 水生生物学报, 2008, 32(1): 33—37 http://ssswxb.ihb.ac.cn/CN/abstract/abstract3473.shtml
    [4]
    Liu D, Wu X, Chang J, et al. Constructed wetlands as biofuel production systems [J]. Nature Climate Change, 2012, 2(3): 190—194 doi: 10.1038/nclimate1370
    [5]
    吴晓磊. 人工湿地废水处理机理. 环境科学, 1995, 16(3): 83—86 http://www.cnki.com.cn/Article/CJFDTOTAL-FJNJ200902005.htm

    Wu X L. Mechanism of wetland treatment in constructed wetland [J]. Environment Science, 1995, 16(3): 83—86
    吴晓磊. 人工湿地废水处理机理. 环境科学, 1995, 16(3): 83—86 http://www.cnki.com.cn/Article/CJFDTOTAL-FJNJ200902005.htm
    [6]
    Liu D, Ge Y, Chang J, et al. Constructed wetlands in China: recent developments and future challenges [J]. Frontiers in Ecology and the Environment, 2008, 7(5): 261—268
    [7]
    Greenway M. Suitability of macrophytes for nutrient removal from surface flow constructed wetlands receiving secondary treated sewage effluent in Queensland, Australia [J]. Water Science and Technology, 2003, 48(2): 121—128
    [8]
    陈长太, 王雪, 祁继英. 国外人工湿地技术的应用及研究进展. 中国给水排水, 2003, 19(12): 105—106 doi: 10.3321/j.issn:1000-4602.2003.12.036

    Chen C G, Wang X, Qi J Y. Application and study advances of artificial wetland technology in foreign [J]. China Water & Wastewater, 2003, 19(12): 105—106
    陈长太, 王雪, 祁继英. 国外人工湿地技术的应用及研究进展. 中国给水排水, 2003, 19(12): 105—106 doi: 10.3321/j.issn:1000-4602.2003.12.036
    [9]
    赵晶, 赵和平, 许良峰, 等. 复合人工湿地对水源地库区水质净化效果分析. 环境工程学报, 2013, 7(12): 4816— 4822 http://www.cnki.com.cn/Article/CJFDTOTAL-HJJZ201312039.htm

    Zhao J, Zhao H P, Xu L F, et al. Analysis of water purification efficiency of integrated constructed wetland in drinking water supply reservoir [J]. Chinese Journal of Environmental Engineering, 2013, 7(12): 4816—4822
    赵晶, 赵和平, 许良峰, 等. 复合人工湿地对水源地库区水质净化效果分析. 环境工程学报, 2013, 7(12): 4816— 4822 http://www.cnki.com.cn/Article/CJFDTOTAL-HJJZ201312039.htm
    [10]
    Zhu H Y, Zhou M, Liu X. Application of Artificial Wetland Technology in Rural Water Pollution Control [C]. Advanced Materials Research. Trans Tech Publications. 2014, 717—720
    [11]
    王加鹏, 崔正国, 周强, 等. 人工湿地净化海水养殖外排水效果与微生物群落分析. 渔业科学进展, 2014, 35(6): 1—9 doi: 10.11758/yykxjz.20140601

    Wang J P, Cui Z G, Zhou Q, et al. Removal effect of mariculture wastewater and analysis of microbial communities in constructed wetlands [J]. Progress in Fishery Sciences, 2014, 35(6): 1—9
    王加鹏, 崔正国, 周强, 等. 人工湿地净化海水养殖外排水效果与微生物群落分析. 渔业科学进展, 2014, 35(6): 1—9 doi: 10.11758/yykxjz.20140601
    [12]
    Farzadkia M, Ehrampoush M H, Abouee Mehrizi E, et al. Investigating the efficiency and kinetic coefficients of nutrient removal in the subsurface artificial wetland of Yazd wastewater treatment plant [J]. Environmental Health Engineering and Management Journal, 2015, 2(1): 23—30
    [13]
    李林锋, 年跃刚, 蒋高明. 人工湿地植物研究进展. 环境污染与防治, 2006, 28(8): 616—620 http://www.cnki.com.cn/Article/CJFDTOTAL-AQHJ201205009.htm

    Li L F, Nian Y G, Jiang G M. Macrophytes in constructed wetland for wastewater treatment [J]. Environment Pollution & Control, 2006, 28(8): 616—620
    李林锋, 年跃刚, 蒋高明. 人工湿地植物研究进展. 环境污染与防治, 2006, 28(8): 616—620 http://www.cnki.com.cn/Article/CJFDTOTAL-AQHJ201205009.htm
    [14]
    汤显强, 李金中, 李学菊, 等. 人工湿地不同填料去污性能比较. 水处理技术, 2007, 33(5): 45—48 http://www.cnki.com.cn/Article/CJFDTOTAL-SCLJ200705011.htm

    Tang X Q, Li J Z, Li X J, et al. Comparison between performance of pollutant removal by different packings in constructed wetland [J]. Technology of Water Treatment, 2007, 33(5): 45—48
    汤显强, 李金中, 李学菊, 等. 人工湿地不同填料去污性能比较. 水处理技术, 2007, 33(5): 45—48 http://www.cnki.com.cn/Article/CJFDTOTAL-SCLJ200705011.htm
    [15]
    靖元孝, 杨丹菁, 陈章和, 等. 两栖榕在人工湿地的生长特性及其对污水的净化效果. 生态学报, 2003, 23(3): 614—619 http://www.cnki.com.cn/Article/CJFDTOTAL-STXB200303025.htm

    Jing Y X, Yang D J, Chen Z H, et al. Growth characteris-tics and sewage purifying effect of amphibious banyan in constructed wetland [J]. Acta Ecologica Sinica, 2003, 23(3): 614—619
    靖元孝, 杨丹菁, 陈章和, 等. 两栖榕在人工湿地的生长特性及其对污水的净化效果. 生态学报, 2003, 23(3): 614—619 http://www.cnki.com.cn/Article/CJFDTOTAL-STXB200303025.htm
    [16]
    Bolton K G E, Greenway M. Pollutant removal capability of a constructed Melaleuca wetland receiving primary settled sewage [J]. Water Science and Technology, 1999, 39(6): 199—206 doi: 10.1016/S0273-1223(99)00140-7
    [17]
    胡焕斌, 周化民, 王桂珍, 等. 人工湿地处理矿山炸药污水. 环境科学与技术, 1997, (3): 17—18 http://www.cnki.com.cn/Article/CJFDTOTAL-FJKS199703005.htm

    Hu H B, Zhou H M, Wang G Z, et al. Mining explosives sewage was treated by constructed wetland [J]. Environmental Science & Technology, 1997, (3): 17—18
    胡焕斌, 周化民, 王桂珍, 等. 人工湿地处理矿山炸药污水. 环境科学与技术, 1997, (3): 17—18 http://www.cnki.com.cn/Article/CJFDTOTAL-FJKS199703005.htm
    [18]
    Haberl R, Perfler R, Mayer H. Constructed wetlands in Europe [J]. Water Science and Technology, 1995, 32(3): 305—315 doi: 10.1016/0273-1223(95)00631-1
    [19]
    Bankston J L, Sola D L, Komor A T, et al. Degradation of trichloroethylene in wetland microcosms containing broad-leaved cattail and eastern cottonwood [J]. Water Research, 2002, 36(6): 1539—1546 doi: 10.1016/S0043-1354(01)00368-2
    [20]
    金相灿, 贺凯, 卢少勇, 等. 4种填料对氨氮的吸附效果. 湖泊科学, 2008, 20(6): 755—760 doi: 10.18307/2008.0613

    Jin X C, He K, Lu S Y, et al. Adsorption effect of ammonia by four fillings [J]. Journal of Lake Sciences, 2008, 20(6): 755—760
    金相灿, 贺凯, 卢少勇, 等. 4种填料对氨氮的吸附效果. 湖泊科学, 2008, 20(6): 755—760 doi: 10.18307/2008.0613
    [21]
    张修稳, 李锋民, 卢伦, 等. 10 种人工湿地填料对磷的吸附特性比较. 水处理技术, 2014, 40(3): 49—52 http://www.cnki.com.cn/Article/CJFDTOTAL-SCLJ201403014.htm

    Zhang X W, Li F M, Lu L, et al. The adsorption properties of ten CW substrates on phosphorus [J]. Technology of Water Treatment, 2014, 40(3): 49—52
    张修稳, 李锋民, 卢伦, 等. 10 种人工湿地填料对磷的吸附特性比较. 水处理技术, 2014, 40(3): 49—52 http://www.cnki.com.cn/Article/CJFDTOTAL-SCLJ201403014.htm
    [22]
    Li J, Pan Y. Environmental factors affect magnetite magnetosome synthesis in Magnetospirillum magneticum AMB-1: implications for biologically controlled minera-lization [J]. Geomicrobiology Journal, 2012, 29(4): 362—373 doi: 10.1080/01490451.2011.565401
    [23]
    关松荫. 土壤酶及其研究法. 北京: 农业出版社. 1986, 294—311

    Guan S Y. Soil Enzyme and Method [M]. Beijing: Agriculture Press. 1986, 294—311
    关松荫. 土壤酶及其研究法. 北京: 农业出版社. 1986, 294—311
    [24]
    Dierberg F E, DeBusk T A, Jackson S D, et al. Submerged aquatic vegetation-based treatment wetlands for removing phosphorus from agricultural runoff: response to hydraulic and nutrient loading [J]. Water Research, 2002, 36(6): 1409—1422 doi: 10.1016/S0043-1354(01)00354-2
    [25]
    Verhoeven J T A, Meuleman A F M. Wetlands for wastewater treatment: opportunities and limitations [J]. Ecological Engineering, 1999, 12(1): 5—12
    [26]
    Constructed Wetlands and Aquatic Plant Systems for Municipal Wastewater Treatment [M]. US Environmental Protection Agency, Office of Research and Development. 1988
    [27]
    Bai J H, Ouyang H, Deng W, et al. A review on nitrogen transmission processes in natural wetlands [J]. Acta Ecologica Sinica, 2005, 25(2): 326—333
    [28]
    Lu S Y, Zhang P Y, Yu G, et al. Stabilization pond-plant bed composite system treatment of farmland irrigation and drainage water [J]. China Environmental Science, 2004, 24(5): 605—609
    [29]
    卢少勇, 金相灿, 余刚. 人工湿地的磷去除机理. 生态环境, 2006, 15(2): 391—396 http://www.cnki.com.cn/Article/CJFDTOTAL-TRYJ200602040.htm

    Lu S Y, Jin X C, Yu G. Phosphorus removal mechanism of constructed wetland [J]. Ecology and Environment, 2006, 15(2): 391—396
    卢少勇, 金相灿, 余刚. 人工湿地的磷去除机理. 生态环境, 2006, 15(2): 391—396 http://www.cnki.com.cn/Article/CJFDTOTAL-TRYJ200602040.htm
    [30]
    郭萧, 熊飞, 赵安娜, 等. 植物配置对表层流湿地净化效果的影响研究. 环境污染与防治, 2010, (5): 57—60 http://www.cnki.com.cn/Article/CJFDTOTAL-HJWR201005015.htm

    Guo X, Xiong F, Zhao A N, et al. Effect of plant disposition on purification efficiency of surface flow wetlands [J]. Environment Pollution & Control, 2010, (5): 57—60
    郭萧, 熊飞, 赵安娜, 等. 植物配置对表层流湿地净化效果的影响研究. 环境污染与防治, 2010, (5): 57—60 http://www.cnki.com.cn/Article/CJFDTOTAL-HJWR201005015.htm
    [31]
    陆松柳, 张辰, 徐俊伟. 植物根系分泌物分析及对湿地微生物群落的影响研究. 生态环境学报, 2011, 20(4): 676—680 http://www.cnki.com.cn/Article/CJFDTOTAL-TRYJ201104016.htm

    Lu S L, Zhang C, Xu J W. Root exudates of wetland plants and the influence on the microbial community in constructed wetlands [J]. Ecology and Environmental Sciences, 2011, 20(4): 676—680
    陆松柳, 张辰, 徐俊伟. 植物根系分泌物分析及对湿地微生物群落的影响研究. 生态环境学报, 2011, 20(4): 676—680 http://www.cnki.com.cn/Article/CJFDTOTAL-TRYJ201104016.htm
    [32]
    岳春雷, 常杰, 葛滢, 等. 复合垂直流人工湿地基质酶活性及其与水质净化效果之间的相关性. 湖泊保护与生态文明建设—第四届中国湖泊论坛论文集. 2014

    Yue C L, Chang J, Ge Y, et al. Spatial distribution of enzymatic activity in substrate of constructed wetland and its correlation with purification of wastewater [C]. Lake Protection and Ecological Civilization—Paper of Fourth Forum on China Lake. 2014
    岳春雷, 常杰, 葛滢, 等. 复合垂直流人工湿地基质酶活性及其与水质净化效果之间的相关性. 湖泊保护与生态文明建设—第四届中国湖泊论坛论文集. 2014
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